| |
Cell numbers/sample (×103)
|
Absorbance/sample
|
Absorbance/10,000 cells
|
|---|
| |
Control
|
Osteogenic
|
Control
|
Osteogenic
|
Control
|
Osteogenic
|
|---|
|
Nonprimed ALPL+
|
13.5 ± 4.2
|
11.2 ± 9.0
|
0.21 ± 0.10
|
0.80 ± 0.21
|
0.15 ± 0.04
|
2.71 ± 4.76
|
|
Primed ALPL+
|
9.5 ± 3.2
|
11.0 ± 4.0
|
0.14 ± 0.03
|
1.03 ± 0.87
|
0.16 ± 0.04
|
0.98 ± 0.64
|
|
Unsorted
|
11.5 ± 3.4
|
15.0 ± 8.7
|
0.16 ± 0.05
|
0.36 ± 0.19
|
0.14 ± 0.03
|
0.60 ± 0.81
|
|
Primed ALPL–
|
7.4 ± 1.6
|
14.0 ± 4.7
|
0.11 ± 0.02
|
0.19 ± 0.04
|
0.15 ± 0.02
|
0.14 ± 0.03
|
|
Nonprimed ALPL–
|
9.2 ± 2.5
|
13.0 ± 5.5
|
0.13 ± 0.03
|
0.28 ± 0.22
|
0.15 ± 0.03
|
0.19 ± 0.11
|
- Primed indicates cells received osteogenic differentiation medium prior to alkaline phosphatase liver/bone/kidney (ALPL) sorting. Unsorted population was primed. Data for control and osteogenic media conditions are shown (mean ± standard deviation).
