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Figure 2 | Stem Cell Research & Therapy

Figure 2

From: Successful nucleofection of rat adipose-derived stroma cells with Ambystoma mexicanum epidermal lipoxygenase (AmbLOXe)

Figure 2

Expression of AmbLOXe in the nucleofected adipose-derived stroma cells . Ambystoma mexicanum epidermal lipoxygenase (AmbLOXe) expression was detected by polymerase chain reaction in the nucleofected cell populations 5 days after transfection. In pIRES-EGFP-AmbLOXe (pI-AmbLOXe) nucleofected cells, pI-AmbLOXe/pmaxGFP® co-nucleofected cells (ratio 1:1) as well as in the positive control (pI-AmbLOXe vector), a band corresponding to the size of full-length AmbLOXe could be detected (lanes 4 to 6). No bands were observed after nucleofection without DNA or with pmaxGFP® alone (lanes 2 and 3). Lane 1, DNA ladder; lane 2, nucleofection without DNA; lane 3, nucleofection with pmaxGFP®; lane 4, nucleofection with pI-AmbLOXe; lane 5, nucleofection with pI-AmbLOXe/pmaxGFP®; lane 6, pI-AmbLOXe alone as vector (positive control); lane 7, water (negative control).

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Stem Cell Research & Therapy

ISSN: 1757-6512