Figure 2

Adipogenic and osteogenic differentiation of hES-derived mesenchymal cells. (a) Analysis of osteogenic activity of EDK, H9-MSC, and human foreskin fibroblasts (HFF). Osteogenic differentiation was induced using the β-glycerophosphate method, after four weeks of differentiation, calcium deposition was stained with alzarin red. Bars, 50 μm. (b) Quantification of alizarin red staining (t-test: **P < 0.01). Results are expressed as the mean +/- standard deviation (SD) of three independent experiments and three technical replicates per experiment. (c) Analysis of adipogenic activity of EDK, H9-MSC, and HFF. Adipogenic differentiation was induced using the 3-isobutyl-1-methylxanthine (IBMX) method, and after four weeks of differentiation the lipid vesicles were stained with oil red O. Bars, 50 μm. (d) Quantification of oil red O staining (t-test: **P < 0.01). Results are expressed as the mean +/- SD of three experiments and three technical replicates per experiment.