Figure 2
Cytokine preconditioning results in increased type VII collagen protein secretion. Mesenchymal stem cells (MSCs) were treated with 15 ng/ml transforming growth factor beta +30 ng/ml tumor necrosis factor alpha for 48 hours. Following this incubation period, culture medium was extracted and subjected to sandwich enzyme-linked immunosorbent assay (ELISA) analysis. Culture medium from treated groups was compared with that of untreated MSCs. ELISA experiments were carried out across two biological groups per condition (105 cells per group). Data presented as mean ± standard deviation. *P <0.005 by Student’s t test. C7, type VII collagen protein.