Figure 6

Human NSC transplantation increases BNDF expression in the ipsilesional hemisphere. (A) Representative Western blot showing BDNF expression in the cortex and hippocampus. (B) Quantification of A. (n = 4; **P <0.01, ***P <0.001 versus sham; ^##P <0.01, ^###P <0.001 versus MCAO/R). (C) Representative confocal photomicrographs showing BDNF (red) immunofluorescence in the ipsilesional hippocampus. DAPI staining is shown in blue (c, d: High magnification of rectangles shown in a, b, respectively). Scale bar, 100 μm (a, b); 30 μm (c, d). (D) BDNF-positive endogenous cells (red), Stem121-positive hNSCs (green), and BDNF-positive hNSCs, as indicated by arrow (arrow, yellow; merge). Scale bar, 30 μm. BNDF, brain-derived neurotrophic factor; DAPI, 4′,6-diamidino-2-phenylindole; MCAO/R, middle cerebral artery occlusion with reperfusion; NSC, neural stem cell.