Figure 6
Cell therapy effects in inflammatory infiltrates and demyelination in the spinal cord of experimental autoimmune encephalomyelitis mice. Representative lumbar spinal cord sections from experimental autoimmune encephalomyelitis (EAE) mice, stained with anti-CD3 or anti-myelin basic protein (anti-MBP) antibodies to detect inflammatory infiltrates (A) and the degree of demyelination (B) in both chronic progressive experimental autoimmune encephalomyelitis (CP-EAE)-induced and relapsing–remitting experimental autoimmune encephalomyelitis (RR-EAE)-induced animals. Magnification: (A) 10× and (B) 4×. Graphs show the mean ± standard error of the mean of the number of T cells stained with CD3 in 200 μm2(A), or the demyelinated area, characterized by a lack of MBP staining related to the total area (B), from three sections of six animals per each experimental group. t test: ***P <0.0005 vs. saline. Ad-MSC, adipose tissue-derived mesenchymal stem cell; DPI, days post immunization.