Stem cell marker expression by adipose stem cells. Stem cells isolated from lipoaspirates (L) (A, D, G) and from lipoaspirates enriched with stem cells (L + SC) (B, E, H) express Oct-4 (green fluorescence, A, B, C), Nanog (magenta fluorescence, D, E, F) and c-Kit (red fluorescence, G, H, I). Nuclei are depicted by the blue fluorescence of 4′,6-diamidino-2-phenylindole. (J) Representative surface immunophenotype of adipose stem cells: histogram overlay shows isotype control IgG staining profile (red histograms) versus specific antibody staining profile (green histograms). (K) Population doubling time. (C, F, I, K) Results expressed as mean ± standard deviation. *P <0.05 vs. L. Cytori, Cytori Celution System (Cytori Ltd., Deeside, UK) ; Fastem, Fastem Corios (CORIOS Soc. Coop, San Giuliano Milanese (MI), Italy); Lipokit, Lipokit Medikhan System (Medikan International Inc, Pusan, Korea) .