Figure 2
Use of platelet lysate differentiates pluripotent stem cells towards a mesenchymal phenotype. (A) Scheme of the differentiation protocol used in this work. (B) Microscopic view of the cell’s phenotype progression during protocol. Cells progressively gain cytoplasm and separate from each other. (C) After protocol completion, cells are positively stained for mesenchymal stem cell surface markers, analyzed by flow cytometry. Red, cells stained for the indicated marker; black, unstained control. PL, platelet lysate.