Platelet lysate supplement increases the yield of mesenchymal stem cells derived. Initially, 1.5 × 105 pluripotent stem cells were plated and cultured in medium supplemented either with 10% fetal bovine serum (FBS) or 10% platelet lysate (PL). At the indicated days, cells were passed and counted in a flow cytometer. From day nine onwards, PL supplement generates substantially more cells than FBS (*P <0.05). PD-MSCFBS, pluripotent-derived mesenchymal stem cells supplemented with fetal bovine serum; PD-MSCPL, pluripotent-derived mesenchymal stem cells supplemented with platelet lysate.