Figure 1

Schematic of plasmids pc3.5 and pmaxCDK. Unique restriction sites bordering various genetic elements of the plasmid are shown for pc3.5 (top) or for pmaxCDK (bottom). Various defined genetic elements such as promoters, polyadenylation sequences, and antibiotic resistance elements are labeled. Restriction sites bordering important eukaryotic genetic elements are shown. Sites within parentheses are not unique within pc3.5 but become unique upon the following exchanges: neomycin ORF to either hygromycin or puromycin ORF (BssH II, Pvu II and Nae I), cytomegalovirus (CMV) promoter to the EF1A or cyclooxygenase (COX)-2 promoter (Spe I), and neomycin ORF to puromycin ORF (Pst I). Restriction sites set in red were introduced; the cryptic introduced Cla I site is set in blue. Middle: each plasmid used in this manuscript is shown, with colored and labeled squares denoting a sequence that was introduced. Elements introduced include the human phosphoglycerate kinase (PGK)-1 genomic promoter (in purple), a truncated human elongation factor-1α (EF1A) promoter (in red, see Figure 6), the human COX-2 genomic promoter (in yellow), the β-globin/IgG synthetic chimeric intron (in pink), the hygromycin resistance gene (in orange), the puromycin ORF (in green), and the human PGK-1 polyadenylation sequence (in blue). BGH, bovine growth hormone; SV40, simian virus-40.