Figure 2

Markers of endoderm, ectoderm and mesoderm differentiation in Activin A treated USSC following directed differentiation. Representative gels depicting mRNA levels of the housekeeping gene GAPDH, endoderm markers Sox17, FOXA2, TTF-1, SPC and CFTR, ectoderm markers Nestin and Pax6 and mesoderm markers MixL1 and Brachyury. mRNA levels were determined in Activin-A treated USSC, low serum control USSC and serum-free controls before (Figure 2a) and following (Figure 2b) differentiation into cells representative of each of the three germline layers. Spontaneously differentiated hESC and human bronchial epithelial cells were used as positive controls and undifferentiated USSC were used as negative controls. + depicts RT positive cDNA, - depicts RT negative controls.