Figure 6

Amyloid precursor protein (APP) is expressed in neural stem cells and neural progenitor cells of the adult subventricular zone (SVZ). (a) Western blot analysis shows higher expression levels of APP in the SVZ of APP(+/+) and APP(+/-) mice compared with the hippocampus and cerebellum. (b) Levels of sAPP are dramatically higher in the SVZ of APP(+/+) and APP(+/-) mice compared with the hippocampus and cerebellum. Optical density of expression level of APP (c) and sAPP (d) in Western blotting shown in (a). (e) Representative confocal images of APP staining in brain sections: Top panels: APP staining in the SVZ of APP(+/+) (left) and APP(-/-) (right) mice. Middle and bottom panels: High-power images of APP colocalization with Sox2 (middle left), nestin (middle right), and Dlx-2 (bottom) in the SVZ of APP(+/+) mice. Error bars represent standard error of the mean. *P < 0.009 (c), *P < 0.01 (d), analysis of variance with post hoc analysis. Scale bar = 250 μm. A.U., arbitrary units.