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Table 2 Quantitative real-time PCR analysis of expanded human MSCs

From: A novel serum-free medium for the expansion of human mesenchymal stem cells

qRT-PCR SFM SCM
Target Expression ΔCt Expression level ΔCt
Positive Human MSC markers
CD44 N/A N/A N/A N/A
CD73 ++++ 2.55 ++++ 3.53
CD90 ++++ 2.40 ++++ 2.63
CD105 ++++ 2.63 ++++ 1.82
Nestin +++ 5.79 ++ 11.17
  1. Quantitative real-time PCR analysis of known positive human MSC surface antigens for cells expanded in both SFM and SCM after four passages. ΔCt, Ct (gene-of-interest) - Ct (GAPDH), ++++, very high; +++, high; ++, moderate; +, low.