Figure 1

Characterization of human induced pluripotent stem cells generated from patient-specific keratinocytes. Patient-specific induced pluripotent stem (iPS) cell clones were generated from keratinocytes of Wilms tumor (WT), systemic lupus erythematosus (SLE) and autosomal-dominant polycystic kidney disease (ADPKD) patients by ectopic expression of lentiviral vectors expressing OCT3/4, SOX2, KLF4 and c-MYC. iPS cell clones from keratinocytes of the WT patient were termed WT-iPS, similarly for SLE-iPS and ADPKD-iPS. Two clones each were analyzed for human embryonic stem (ES) characteristics (WT#5J, WT#20G, SLE#100H, SLE#20J, ADPKD#50B and ADPKD#10D). WT-iPS, SLE-iPS and ADPKD-iPS clones were generated and maintained under feeder-free conditions, exhibiting morphology similar to human ES cells. (A) All of the iPS cell clones expressed alkaline phosphatase (AP). (B) WT-iPS, SLE-iPS and ADPKD iPS clones expressed cell surface markers SSEA4, TRA-1-60 and TRA-1-81, whereas no notable staining was observed for SSEA1. The nucleus was revealed by counterstaining with 4,6-diamidino-2-phenylindole. Phase-contrast images and AP images are 10× magnification. Immunofluorescence images were obtained at 40× magnification.