Figure 4

Wilms tumor and systemic lupus erythematosus induced pluripotent stem cells form teratoma in immunodeficient mice. Wilms tumor (WT)-induced pluripotent stem (iPS) cell, systemic lupus erythematosus (SLE)-iPS cell and autosomal-dominant polycystic kidney disease (ADPKD)-iPS cell clones were injected subcutaneously into SCID-Beige mice. Tumors were detected from the site of injection and harvested after 3 to 5 months, examined for the presence of cells of three embryonic germ layers. H & E-stained teratoma showed multiple differentiated tissues including cells of ectoderm, neuronal rosette-like structures (indicated *), endoderm (gut tube-like structures, intestinal epithelial cells) and mesoderm (adipose-like tissue, muscle-like tissue). Magnification 10×.