Figure 2

Subretinal technique. In this technique, a cannula is connected to a 1-mL tuberculin syringe (with a screw plunger) containing balanced salt solution (BSS). The BSS is injected slowly to create a retinotomy, and a small subretinal bleb is raised (A). This procedure minimizes retinal trauma. The cannula is introduced through the retinotomy, and the BSS injection restarts and continues to expand the bleb to the correct volume. A process of gentle retinal massage is used to release the tension in the bleb. The cannula is removed, and a 30-gauge cannula, connected to sterile tubing and syringe preloaded with cells, is introduced in the subretinal space (B).