Figure 1

The combination of IL-1β and TGF-β induces neurosphere formation of LN-229 cells. (A) Representative images of LN-229 cells in the absence or presence of IL-β and TGF-β. LN-229 cells (10,000 cells/well) were cultured in six-well plates containing serum-free medium (SFM) in the absence or presence of 200 pM IL-1β or/and 200 pM TGF-β for seven days. Scale bar = 200 μm. (B-C) Neurosphere formation and proliferation of LN-229 cells cultured at a clonal density (1 cell/μl) in 96-well plates containing 100 μl SFM in each well with or without IL-1β/TGF-β for 14 days. (B) The percentage of newly formed neurospheres per well in the presence IL-1β/TGF-β. *P < 0.05. (C) IL-1β/TGF-β-induced neurosphere cells proliferated more slowly than control cells, as demonstrated using an MTT assay. Error bars represent SEM. *P < 0.05.