Figure 4

mRNA expression by BM-derived MSCs cultured in different expansion media. Reverse transcription-polymerase chain reaction (RT-PCR) for positive control GAPDH (A), osteogenic (B), adipogenic (C), and vascular smooth muscle (D) gene expression of BM-derived MSCs cultured in four media: M1 (10% FBS + FGF2), M2 (10% FBS + 5% HPL), M3 (10% HPL), and M4 (5% HPL). Total RNA was extracted from undifferentiated and differentiated MSCs previously cultured in four expansion media (M1, M2, M3, and M4). The cDNA obtained was used for PCR assays to evaluate the expression of the different genes studied. One representative experiment out of four experiments is shown. For GAPDH gene expression (A): 1, at D0 before any differentiation; 2, after osteogenic differentiation (D14); 3, after adipogenic differentiation (D14); 4, after vascular smooth muscle differentiation (D14). For osteogenic (RUNX2 and ALP) (B), adipogenic (PPARγ and LPL) (C), and vascular smooth muscle (ASMA) (D) gene expression: 1, D0 before any differentiation; 2, after differentiation induction (D14). ALP, alkaline phosphatase; ASMA, alpha-smooth muscle actin; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; LPL, lipoprotein lipase; PPARγ, peroxisome proliferator-activated receptor-γ; RUNX2, runt-related transcription factor 2.