Fig. 3

Characterization of sorted CD146 populations undergoing osteoblast (OB) differentiation. a Reverse transcription-polymerase chain reaction data (mean ± standard error of the mean, n = 3 independent experiments) analyzed from sorted population at day (d) 0, 5, 10, and 15. *P < 0.05. sh = short form and lg = long form of CD146. b Alkaline phosphatase (ALP) activity/cell viability at d6 of osteoblastic differentiation. c ALP staining at d6 and d10 of OB differentiation. Alizarin red (AZR) staining at d10 and d15 of OB differentiation. n = 3 independent experiments. white box, hMSC-CD146⁺; black box, hMSC-CD146⁻; gray box, lgCD146⁺