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Erratum to: Viability of equine mesenchymal stem cells during transport and implantation
Stem Cell Research & Therapy volume 7, Article number: 161 (2016)
- The original article was published in Stem Cell Research & Therapy 2014 5:1
Following the publication of our article  the following minor errors and omissions were brought to our attention.
Throughout the manuscript, reference should have been made to Bone Marrow Supernatant (BMS) and not Bone Marrow Aspirate (BMA).
Materials and Methods
Assays using Annexin V and propidium iodide were performed on separate samples (replicates) and not as repeated measures, a point that was not made clear in the original article. Separate aliquots of cells for each time point were kept in culture, without media changes, until assayed.
Cell proliferation assay and Metabolic activity
In alamarBlue® experiments relating to transport media, we used the interpretation of proliferation because cells were cultured over several days, allowing time for cell proliferation to take place. In contrast, when interpreting viability assays following needle extrusion, we employed a metabolic activity interpretation because the cells were cultured for a short period (24 h, Fig. 5) when cell proliferation would not be a prominent feature. Measurements in both instances were of fluorescence.
Figure 5: The y-axis is incorrectly labelled as “Absorbance”. The correct axis label should read “% non-injected control” and is based on fluorescence readings. The correct figure is provided here (Fig. 5).
Where appropriate, all figure legends in our article should state that error bars represent the standard error of the mean (± SEM).
These corrections do not alter the results or conclusion of the study that (i) the medium used for transportation of bone marrow mesenchymal stem cells had a significant impact on cell viability with increasing transport time and (ii) the use of a larger needle size (19G) for cell implantation reduced stress-induced loss of viability compared to smaller needle gauges (21G and 23G).
Garvican ER, Cree S, Bull L, Smith RKW, Dudhia J. Viability of equine mesenchymal stem cells during transport and implantation. Stem Cell Res Therapy. 2014;5:94.
The online version of the original article can be found under doi:10.1186/scrt483.
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Garvican, E.R., Cree, S., Bull, L. et al. Erratum to: Viability of equine mesenchymal stem cells during transport and implantation. Stem Cell Res Ther 7, 161 (2016). https://doi.org/10.1186/s13287-016-0423-z