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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis

Fig. 5

Initial cell number modulates the expression of different mechanosensing proteins. a Western blot analysis revealed that initial cell number significantly impacts the expression of Cav-1, N-cad, β-cat, and FN. b Quantification of western blot analysis data. Values are normalized to GAPDH. Statistical analysis is based on a one-way ANOVA tool of OriginPro. Error bars are standard error of the mean, and different aggregates in one group are compared to 70 k in the same group (*p < 0.05, **p < 0.01, ***p < 0.001). c Immunofluorescence staining for Cav-1 and N-cad in sections of pellets after 7 days of differentiation

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