ACN activates and modulates early phase of chondrogenic differentiation in MSCs independent of exogenous soluble TGF-β1
Since it has been suggested that in limb mesenchyme prior to condensation contains endogenous TGF-β1 [32], we first inquired, if ACN by itself is capable of inducing the expression of glycosaminoglycans (GAGs), an early sign of chondrogenesis, even in the absence of exogenous TGF-β1. In order to ascertain this, we employed the well-established 3D aggregate culture system [27] and cultured MSCs aggregates with different ACNs (70 k, 150 k, 250 k, 350 k, and 500 k) for 7 and 21 days in the absence of TGF-β1, and we observed that the lowest ACNs of 70 k showed adequate staining of GAGs and ACN of 150 k showed mild staining for GAGs even at day 7. However, above this ACN, low to no GAGs expression was observed (Fig. 1a). In presence of TGF-β1, the expression of GAGs was enhanced as expected, but again it was most pronounced in ACN of 70 k and 150 k, and the trend of decreasing GAGs expression with increasing ACN was maintained (Fig. 1b). It is noteworthy that the expression of GAGs on day 7 was observed both in the absence and presence of TGF-β1 in ACN of 70 k, thus providing the first evidence that the activation of the chondrogenic program in MSCs upon condensation is independent of soluble TGF-β1 and it is an intrinsic mechanism. However, on day 21, the +TGF-β1 condition showed a substantial increase in the quantity (size of cartilaginous mass, Fig. 1b) and quality of the GAG matrix; however, the impact of ACN on the organization and shape of the cells and the uniformity and intensity of the GAG matrix was still evident (Fig. 1a, b; Additional file 1: Figure S1).
Lower ACN enhances matrix production during MSC chondrogenesis in a TGF-β1-dependent manner
We then proceeded to quantify the quality of the cartilaginous matrix using the Bern score, an accepted semi-quantitative metric to describe chondrogenesis [28]. In this scoring system, three factors go into assessing the quality of the cartilage matrix: (1) cell morphology, (2) distance between cells/amount of matrix produced uniformity, and (3) intensity of Alcian Blue staining, and each factor is given a score ranging from 0 to 3. Thus, aggregates with highest chondrogenic differentiation have a score of 9, and the ones with the least have a score of 0. The histological analysis and Bern score assessment of aggregates at day 7 of chondrogenic differentiation revealed that addition of TGF-β1 does not alter the Bern score of aggregates significantly (Fig. 1c). In both conditions (± TGF-β1), cells within the low-ACN aggregates particularly in the periphery had already acquired chondrogenic morphology (rounded) and showed expression of GAGs with a Bern score in the range of ~ 3. In comparison, high-ACN conditions promoted a fibroblastic morphology and showed little-to-no GAG production throughout the aggregate cross-section and had a negligible Bern score. This is a significant observation as it suggests that in the early stages of MSCs-derived chondrogenesis, intrinsic signaling plays a dominant role. However, at day 21, although the clear trend of higher chondrogenesis at lower ACN was sustained, the Bern score was consistently higher as expected in presence of TGF-β1. This trend between ACN and GAGs expression was also confirmed in other donors (age 25–56 years old male and female) confirming the generality of the observations (Additional file 1: Figure S2).
Visual inspection of MSCs aggregates using light microscopy revealed that in presence of TGF-β1, despite the fact that the lowest ACN condition (70 k) (low-ACN) had seven times less cells at the onset compared to the highest ACN (500 k) (high-ACN), after 7 days, the volumetric size difference among aggregates was negligible, and after 21 days, the diameter of the high-ACN aggregates was only around 1.5 times of the low-ACN aggregates (Fig. 1d). However, such a dramatic change from day 7 to day 21 was not present in absence of TGF-β1, again confirming that ACN acts as a trigger for chondrogenic differentiation of MSCs; however, matrix production requires exogenous signals. This led us to postulate that either ECM production in aggregates with low-ACN was more efficient, and/or cells within this environment were able to undergo proliferation. Since the effect of ACN was preserved even in presence of TGF-β1, all further analysis was undertaken in presence of TGF-β1.
ACN impacts the expression of genes regulating chondrogenesis and endochondral ossification
To investigate the impact of ACN on gene expression pattern in MSCs, we employed Affymetrix gene array analysis. Cells were isolated from pellets formed in presence of TGF-β1 48 h past initiation of chondrogenic differentiation. A heat map showing the top 100 differentially regulated genes as a function of initial ACN is shown in Fig. 2. Noteworthy, genes playing a very important role in chondrogenesis such as parathyroid hormone-1 receptor (PTH1R) [33] was upregulated by more than sixfold in 70 k pellets. Interestingly, genes regulating Wnt/β-catenin signaling cascade such as frizzled class receptor 4 (FZD4) and dickkopf-related protein 1 (DKK1) were significantly (more than threefold) altered in low ACNs. Based on this information, we postulate that the Wnt/β-catenin signaling pathway could be one of the main modulators of ACN-induced chondrogenesis. Additionally, genes such as integrin-binding sialoprotein (IBSP), alkaline phosphatase (ALPL), bone morphogenetic protein 4 (BMP4), pannexin 3 (PANX3), asporin (ASPN), Indian hedgehog (IHH), and hes family bHLH transcription factor 1 (HES1) with a role in development of cartilage and EO were highly upregulated in low ACNs. Interestingly, genes associated with cellular response to lipids (GO:0071396) such as bradykinin receptor B1 (BDKRB1), argininosuccinate synthase 1 (ASS1), high mobility group box 2 (HMGB2), interleukin-1 receptor-associated kinase 2 (IRAK2), phosphodiesterase 4D (PDE4D), Kruppel-like factor 9 (KLF9), nuclear receptor subfamily 4, group A, member 3 (NR4A3), and tumor necrosis factor receptor superfamily, member 1B (TNFRSF1B) were all highly downregulated in low-ACNs.
Upon analysis of the top 300 differentially regulated genes (min 2.2-fold), we found out that genes involved in regulation of lipid storage (GO:0010883) such as caveolin-1 (CAV1), interleukin 6 (IL6), CD36, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor (NFKBIA), and integrin beta 3 (ITGB3) and regulation of lipid biosynthetic process (GO:0046890) and regulation of lipid transport (GO:0032368) such as nuclear receptor subfamily 1, group D, member 1 (NR1D1), leptin (LEP), complement component 3 (C3), and salt-inducible kinase 1 (SIK1) were all downregulated in low-ACNs.
Additionally, we did not see significant differences in expression of hypoxia-associated genes such as hypoxia-inducible factors 1 and 3 (HIF1A, HIF3A), egl-9 family hypoxia-inducible factor 3 (EGLN3) [34], and hypoxia-inducible lipid droplet-associated (HILPDA) between MSCs from various ACNs. This was an interesting observation as it has been widely accepted that multicellular tumor spheroids experience hypoxia [35, 36] and the diffusion of nutrition and oxygen in multicellular aggregates is compromised in direct relation to the aggregates size [37].
Modeling of oxygen and TGF-β1 diffusion and glucose consumption in different ACNs
Oxygen availability has been frequently suggested as one of the primary factors influencing MSC fate [38]. It has been reported that differentiating embryoid bodies experience gradients of nutrients, oxygen, and cytokines and that the concentration of oxygen at the centers of embryoid bodies with a radius of 400 μm was 50% lower than that in embryoid bodies with a radius of 200 μm [39]. Even though in this study aggregates at day 7 of differentiation have a relatively similar size (Additional file 1: Table S1), in order to rule out the impact of oxygen and nutrient diffusion in the observed outcomes, we modeled the diffusion of oxygen, glucose, and TGF-β1 in aggregates of varying ACNs at day 7 of chondrogenic differentiation.
The diffusion of the molecules is modeled as Fick’s law of diffusion, described by the following partial differential equation (PDE):
$$ \frac{\partial c}{\partial t}-D\Delta c+ kc=0 $$
where c = c (x, t) is the compound concentration at location x and time t, D is the diffusion coefficient, ∆ is the Laplace operator, and k is the uptake rate. The data described in Additional file 1: Table S2 was used.
As per modeling, the oxygen concentration at the center of the pellet is expected to be similar to the edge of the pellet with a less than 10% difference observed in the case of the 500 k pellets (Fig. 3a). The differences in oxygen concentration predicted for various ACNs do not constitute hypoxia as even larger variations are observed in human physiology in the range of 3–7% oxygen [40]. Likewise, the drop in the concentration of TGF-β1 is also predicted to be negligible (Fig. 3c). However, the glucose concentration at the center of the 500 k pellets is predicted to be half (~ 2.2 mg/mL) of that in media (4.5 mg/mL), with no such drastic differences at the center of the low-ACN pellets (Fig. 3b). Since glucose is a small molecule, the only factor that can alter its diffusion behavior is consumption rate. Therefore, a study to examine the proliferation status of the MSCs within different pellets was undertaken. Aggregates were formed using MSCs stably expressing Turbo-GFP and cell numbers were quantified at days 2, 7, and 21 after aggregate formation. Surprisingly, cell proliferation showed an inverse correlation with an increase in ACN, with cell numbers by day 21 within low-ACN aggregates being fourfold higher (after normalization to initial cell number) in comparison with high-ACN (Fig. 3d). This correlates with the lower glucose concentration predicted at the interior of 500 k and 350 k aggregates. This observation is quite significant as we have shown that cartilage engineered de novo in skeletally mature rabbits by inducing chondrogenesis from periosteal progenitor cells is hypercellular and therefore capable of undergoing remodeling in vivo as they try to attain cellular homeostasis [41]. However, further experiments are necessary to understand the role of glucose consumption in low-ACNs aggregates in driving chondrogenesis. Aggregate formation has also been reported to induce the expression of hypoxia-inducible factor (HIF)-1α in particular in the interior. Immunostaining of cryosections of aggregates for HIF-1α revealed that while both low- and high-ACNs show global expression of HIF-1α, in aggregates formed from ACN of 350 k and 500 k an increase in expression of HIF-1α in the cells in close proximity of aggregate core is additionally observed (Fig. 3e). Nonetheless, the absence of any remarkable differences in the diffusion of oxygen and TGF-β1 in the various ACN scenarios pointed to possibly others mechanisms for the observed differences in the chondrogenic capacity of the MSCS.
ACN programs MSCs biomechanics with increasing stiffness in cells correlating with higher chondrogenesis
There is strong evidence that the mechanical properties of cells can contribute to fate choices [42]. The mechanical property of the cell is determined by different cellular components such as plasma membrane, cytoskeleton, and organelles [43, 44]. It has been suggested that the presence of cholesterol and poly-saturated fatty acids in the lipid bilayer increases the stiffness [45]. We have recently shown that cell plasma membrane lipid bilayer composition is altered by lipid transfer, and this impacts the deformability of cell membrane [46]. Since several genes associated with lipid transfer and lipid storage were downregulated in low-ACNs, we investigated if these differences at the gene expression level translate into changes in the stiffness of cells. Single cells dissociated from the aggregates were subjected to real-time deformability cytometry (RT-DC) and characterized for size and deformation at days 2 and 7 of chondrogenic differentiation [29]. Qualitatively, we observed a decrease in projected cell size for increasing ACN at day 7 (Fig. 4a) while the size deformation distribution of more than 1000 single cell measurements per condition shows little variation in deformation; the trend of decreasing cell size by increasing ACN was evident (Fig. 4b). A direct comparison using the probability density function and applying an analytical model to extract mechanical properties [47] suggests that 70 k aggregates have a higher elastic modulus than their counterparts, i.e., high-ACN aggregates (Fig. 4c). Additionally, our analyses suggest that at an earlier stage of chondrogenesis (2 days), varying ACN does not significantly impact the cell size. However, after 7 days, cells from low-ACNs were significantly larger, which interestingly correlates with their higher chondrogenic potential (Fig. 4d, left panel). Purple band indicates values from undifferentiated MSCs from 2D culture. Analysis of the elastic Young’s modulus (E) revealed no clear differences between cells from the various ACN conditions on day 2. However, at day 7, cells of 70 k ACN, which are more chondrogenic, were also significantly stiffer than cells from higher ACNs, which show diminished chondrogenicity (Fig. 4d, right panel). Interestingly, the qualitative comparison between Young’s modulus (E) of cells from high-ACNs (250 k and greater) showed similar values to E of undifferentiated MSCs (purple band across graph). This is the first report to link chondrogenesis to an emerging mechano-phenotype in MSCs.
Expression of mechanosensing proteins N-cadherin and caveolin-1 in MSCs aggregates is modulated by ACN
In order to ascertain the mechanism underpinning the regulation of chondrogenesis by ACN, we investigated the expression of proteins involved cell-cell contact. One of the proteins known to inhibit cell-cell contact in epithelial cells is Cav-1 [48]. Cav-1 is the main scaffolding protein residing in the cholesterol-rich membrane micro-domains (caveolae), which has a documented role in mechanotransduction in endothelial cells [49] and also implicated in transduction of mechanical forces across cell-cell junctions via stretch-activated channels [50]. Caveolae have been implicated in the compartmentalization and regulation of many signaling events such as MSC renewal and differentiation (adipogenic and osteogenic) [26], and its expression has been observed during chondrogenesis in the tibiotarsus (avian limb) and in chondrocytes in the vicinity of the proliferating zone within the cartilage [51] Furthermore, Cav-1 knockout mice show an increase in length of growth plate, number of hypertrophic cells, bone size, and stiffness [52, 53]. Notwithstanding, the relevance of Cav-1 in MSC condensation and chondrogenesis remains ill-defined.
Western blot (WB) analysis revealed that as early as 2 days after induction of differentiation Cav-1 expression showed an unambiguous and direct correlation with ACN, with the high-ACN aggregates having the most pronounced expression which after 7 days of differentiation was 4–5-fold higher compared to low-ACN aggregates (Fig. 5a). This is also in agreement with our Affymetrix gene array data, which showed downregulation of CAV1 by 2.4-folds in low-ACN. However, after 21 days, a general downregulation of Cav-1 in all conditions was observed with no appreciable differences (Additional file 1: Figure S4). In contrast, N-cad expression showed a completely opposite trend, with low-ACN conditions already showing appreciable expression by day 2 which after 7 days was 2–3-fold higher compared to high-ACN condition, implying that increasing ACNs during MSC aggregate formation has a negative effect on N-cad expression and stabilization (Fig. 5a). This overall trend was confirmed by IF staining that revealed a high punctuate expression of Cav-1 during the condensation phase in high-ACN aggregates and vice versa for low-ACN aggregates (Fig. 5b) and dramatic decrease in N-cad expression going from low- to high-ACNs, with the core of high-ACN aggregates almost devoid of N-cad expression (Fig. 5c). This positive correlation between N-cad expression and chondrogenesis in human MSCs is consistent with the findings in avian limb chondrogenesis where perturbation of N-cad function was found to inhibit cellular condensation and chondrogenesis [21, 22]. Interestingly, in murine skeletal myoblasts, the dynamic assembly of N-cad at cell-cell contact involves lipid rafts in particular caveolae [54]. Since it is accepted that Cav-1 has a role in contact inhibition where redistribution of Cav-1 from punctate region on the cell surface to nodes at cell-cell junctions occurs [48, 55], our finding that in human MSCs that N-cad and Cav-1 expression shows inverse relationship therefore alludes to a possible negative regulatory effect of Cav-1 on N-cad expression and stabilization, and a more general role for Cav-1/N-cad interplay in regulating cell function. Additionally, this interesting inverse relationship between two proteins implicated in mechanotransduction provides circumspect evidence for two opposing mechanobiology mechanisms responsible for directing chondrogenesis in MSCs.
ACN influences TCF/LEF transcriptional activity and expression of N-cad and Cav-1 is MSCs
Another important actor in MSC differentiation is β-catenin, a transcriptional co-activator of the canonical Wnt pathway. The role of Wnt family of secreted glycolipoproteins in many embryonic developmental events is well established [56]. It is known that activation of the canonical Wnt pathway is constituent with chondrogenesis [25, 57, 58]. It has been shown that β-catenin expression during limb bud development is higher between days 1 and 4 suggesting a critical role for β-catenin in MSC condensation phase [25, 59]. It has been shown that the extracellular domain of cadherins forms intercellular bonds with surrounding cells through cadherins, while the intracellular domain of cadherins recruits catenins. Recent investigations have shown that cadherin/catenin complexes could participate in the transduction of mechanical forces during development [15]. Interestingly, we saw no major differences in the overall expression of β-catenin between the various conditions (Fig. 5a) baring the general decrease in total β-catenin expression at day 21, which is expected as chondrogenesis reaches homeostasis (Additional file 1: Figure S4). This led us to postulate that, in low-ACN condition, a mechanism involving stabilization of β-catenin might be responsible for driving chondrogenesis. The fate of β-catenin in the cytoplasm is dictated by the presence of binding partners that prevent its degradation and N-cad complexation with β-catenin protects it from adenomatous polyposis coli (APC)-axin degradation machinery. Upon stabilization by N-cad and reaching a critical concentration, β-catenin can translocate to the nuclei and bind to the transcription factors of the T cell factor/lymphoid enhancer factor (TCF/LEF) family driving gene expression by switching the target genes from stage of activation to transcription. However, Cav-1 can also interact with β-catenin. It has been shown in epithelial cells that an increase in the expression of Cav-1 leads to the recruitment of the majority of β-catenin to the cell membrane, thereby diminishing the availability of β-catenin for complexation with TCF/LEF and inhibiting β-catenin-mediated transcription activity [60]. Also, it has been shown in fibroblasts that Cav-1 expression can modulate Wnt/β-catenin signaling by regulating the intracellular localization of β-catenin [48]. Thus, the competition between cadherins and Cav-1 for this limited pool of β-catenin can regulate the transcriptional activity of β-catenin [60]. Our finding that N-cad expression is significantly upregulated in low-ACN aggregates and that increasing ACN results in upregulation of Cav-1 would imply that in low-ACN conditions, β-catenin stabilization in the cytoplasm and shuttling to the nucleus would be favored. In contrast, in high-ACN environment, the recruitment β-catenin to the cell membrane would be favored limiting its transcriptional activity. Visualization of the expression pattern of β-catenin after 7 days of differentiation revealed two important differences that lend credence to this conclusion. (1) While β-catenin expression pattern was more uniform in low-ACN aggregates, it was heterogeneous in high-ACN aggregates (Fig. 6a), and (2) a higher fraction of cells in low-ACN aggregates showed localization of β-catenin within the nuclei (Fig. 6b, c). These observations in sum imply that the β-catenin transcription activity in low-ACN aggregates should be higher. To investigate this, aggregates were formed using MSCs transfected with commercially available ShRNA (7TGC) to stably express a β-catenin/TCF reporter and then dissociated at different time points and analyzed by flow cytometry for β-catenin transcriptional activity. Transfected MSCs in the absence of nuclear β-catenin expressed only red fluorescence protein; however, upon β-catenin binding to TCF/LEF, green fluorescent protein (GFP) expression is induced [61]. Surprisingly cells in aggregates with lowest ACN showed a threefold increase in the percentage of GFP-positive cells on the second day of differentiation followed by a fivefold increase at day 7 compared to the highest ACN (Fig. 6d). This intriguing finding which correlates well with N-cad expression pattern is consistent with the postulate stated earlier and provides circumspect evidence that in MSCs, in the condensation phase, i.e. up to day 7, the cytoplasmic domains of N-cad interact with β-catenin making stable catenin-cadherin complexes, which then lead to translocation of β-catenin inside the nuclei [62,63,64]. The absence of such differences in β-catenin transcription activity at day 21 would be consistent with the maturation of the chondrogenesis process as a whole (Fig. 6d).
ACN impacts expression and activation of MMP-2, a negative regulator of chondrogenesis
The progression of chondrogenesis is accompanied by ECM deposition and remodeling, where matrix metalloproteinases (MMPs) play a critical role in breaking down the ECM leading to release growth factors and unraveling of cryptic binding sites for cells. It has been observed in chick limb bud-derived MSCs that MMP-2 activation during the pre-condensation step inhibits chondrogenesis by negatively regulating cell adhesion [65]. We therefore analyzed the conditioned media from the aggregates at day 7 and day 21 using gelatin zymography and found that secretion of pro-MMP-2 after 7 days increased dramatically (~ 4–5-fold) with increasing ACN, and high-ACN aggregates showed activation of MMP-2 while in the other aggregates, there was no obvious sign of MMP-2 activation. However, by day 21, differences between the expressions of MMP-2 between samples were almost abolished but activation of MMP-2 was still 4–5-fold higher in high-ACN aggregates (Additional file 1: Figure S5). Considering that increasing ACN negatively impacts chondrogenesis, the direct correlation between Cav-1 expression and expression and activation of MMP-2 with increasing ACN led us to further postulate that upregulation of Cav-1 most likely interferes with MSC condensation during chondrogenic differentiation by impacting ECM remodeling. Interestingly, evidence for an association between Cav-1 and MMP-2 has been found in endothelial cells where it has been shown that MMP-2 co-localizes with Cav-1 on the cell surface and that Cav-1 contains both the proposed receptor and the activator of MMP-2 [66].
Low-ACN promotes expression of fibronectin by MSCs
ECM secreted by cells can have a modulatory role in cell adhesion. FN, a high-molecular-weight glycoprotein, is a key component of the ECM of MSCs, and its expression is upregulated during MSC condensation both in vitro and in vivo [6, 67]. FN possesses cell adhesion domains that play a vital role in mediating cell-cell contact, and it has been suggested that the role of FN in limb bud might be to provide a scaffold for MSCs and promote the formation of pre-cartilage cellular aggregates [6, 68]. FN also supports the deposition of collagen and binding protein for latent TGF-β1 and therefore also has an instructive role in a cartilage matrix formation. WB analysis revealed differences in the expression of fibrillar FN between low-ACN and high-ACN aggregates starting at day 2, with a three- to fourfold higher expression of FN after 7 days of differentiation in low-ACN aggregates compared to high-ACN aggregates (Fig. 5a). By day 21, the pronounced difference was absent and FN expression in aggregates with low-ACN was markedly diminished suggesting a maturation/homeostasis in chondrogenesis (Additional file 1: Figure S4). Since the insoluble fibrillar form of FN that is present in the ECM is assembled from secreted soluble FN through complex cell-mediated process [69,70,71], we therefore quantified the amount of soluble FN in the conditioned media of the aggregates at different time points using an in-house developed ELISA (see the “Materials and methods” section for details). We made a compelling finding that the soluble FN in conditioned media from different aggregates throughout the differentiation phase was in a narrow range (11–24 μg/mL) suggesting some autoregulation; however, when normalized to DNA content within the aggregates (i.e., normalization to cell number), FN secretion in early stages of differentiation (day 2 and day 7) in low-ACN aggregates was ~ 2–3-fold higher compared to high-ACN aggregates (Additional file 1: Figure S6). This finding also correlates with WB analysis of fibrillar FN in the ECM at days 2 and 7 (Fig. 5a). This is the first report showing a direct correlation between the FN secretion capacity of MSC and its potential to undergo chondrogenic differentiation and alludes to the possible use of FN expression as a biomarker for the chondrogenic potential of human MSCs. The FN level in low-ACN aggregates also correlates with higher β-catenin transcriptional activity (Fig. 6, Additional file 1: Figure S7), and this conforms to reports in the literature that FN is a direct target of β-catenin signaling [72, 73]. This is also in agreement with our proliferation data (Fig. 3d) as it has been reported that increased FN levels during acinar development result in overproliferation of mammary epithelial cells and acinar size [74]. Interestingly, a recent study has shown that in rat trabecular meshwork cells, stiffness correlated with the expression of FN [75].
Our finding alludes to a complex mechanism involving activation of the canonical Wnt pathway, changes to Cav-1 and FN expression, and cell stiffness in the regulation of chondrogenesis.
ACN exerts control over MSCs proliferation through regulation of survivin
It has been reported in fibroblasts and epithelial cells that Cav-1 expression suppresses cell proliferation by arresting cells in the G0/G1 phase [76] via the suppression of survivin, a member of the inhibitor of apoptosis proteins [77]. This prompted us to explore the expression of survivin in the context of Cav-1 expression. IF staining of cryosections at day 7 showed that Cav-1 expression was markedly increased with increasing ACN (Fig. 7b), and additionally, regions of low chondrogenicity as indicated by the absence of type-2 collagen expression (Fig. 7a) coincided with regions of high Cav-1 expression. As can be seen in Fig. 7, increasing ACN is accompanied with increasing heterogeneity of Cav-1 expression; this could be rationalized by the fact that MSCs are a heterogeneous population and might respond differently to local microenvironment [78]. Interestingly, the survivin expression pattern showed an inverse relationship with Cav-1 expression pattern (Fig. 7b). High magnification z-stack image from high-ACN aggregates (denoted by an asterisk) revealed that the cells with strong expression of Cav-1 lacked expression of survivin and vice versa (Fig. 7c). Our results provide evidence for the first time for an inverse correlation between Cav-1 and survivin expression in human MSCs during the condensation phase. Importantly, this mechanistic relationship between Cav-1, cadherins, and β-catenin transcriptional activity has parallels in the survival mechanisms of metastatic epithelial tumors [79] and provides impetus to extend findings from tumor development and metastasis to skeletal development.
Toward engineering more efficient constructs for bone and cartilage tissue engineering and regenerative medicine
In the bone and cartilage tissue engineering, acquiring a sufficient number of the cells for in vitro culture or in vivo implantation is costly, time-consuming, and remains the rate-limiting step for clinical translation due to the inverse correlation between MSCs differentiation potential and expansion time. To address this issue, we have investigated if implanting few low-ACN in the collagenous matrix will lead to superior outcomes in comparison with traditional methodology of dispersing cells uniformly throughout the matrix. We implanted four 70 k aggregates 2 days post-culture within the collagen matrix and compared it to the outcomes in a collagen matrix seeded with one million MSCs. After 3 weeks of chondrogenic differentiation, the cells within the aggregates were fully mature hypertrophic chondrocytes and were incorporated in the matrix and the deposited tissue was highly homogenous (Additional file 1: Figure S8a). In spite of the total cell number within the aggregate condition being almost four times lower than the conventional culture condition, the outcomes as assessed by the Bern score, which is indicative of the uniformity of the cartilage matrix, was both high and uniform in the aggregate condition (Additional file 1: Figure S8b). Furthermore, interestingly, the developmentally inspired approach inhibited the formation of the hypoxic and necrotic zone in, which occurs due to diminished oxygen and nutrition diffusion in large constructs. This finding validates the premise of our study and demonstrates the framework for potential translational applications.