Fig. 6
From: Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis
ACN modulates the transcriptional activity of β-catenin. a Immunofluorescence staining of β-catenin in sections of pellets after 7 days of differentiation. b High magnification images of β-catenin staining in 70 k pellets. c High magnification images of β-catenin staining in 500 k pellets. d Flow cytometry analysis of ShRNA-transfected MSCs for β-catenin signaling. Values are normalized to GAPDH. Statistical analysis is based on a one-way ANOVA tool of OriginPro. Error bar represents standard deviation, and different aggregates in one group are compared to 70 k in the same group (*p < 0.05, **p < 0.01, ***p < 0.001)