Fig. 7
From: Cell number in mesenchymal stem cell aggregates dictates cell stiffness and chondrogenesis
Expression of collagen type II, caveolin-1, and survivin is modulated by ACN. a Immunofluorescence staining for collagen type 2 in sections of pellets with different initial cell number after 7 days of differentiation. The area with no expression of collagen type 2 is highlighted by a dashed outline. Scale bar is 200 μm. b Immunofluorescence staining for Cav-1 and survivin in sections of pellets with different initial cell number after 7 days of differentiation. The area with high Cav-1 expression is highlighted by a dashed outline. c Left: composite of a high magnification z-stack image of Cav-1 and survivin staining in the area donated by asterisk (*) in b. Right: focal plane in the middle of the stack