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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Low-density lipoprotein receptor-deficient hepatocytes differentiated from induced pluripotent stem cells allow familial hypercholesterolemia modeling, CRISPR/Cas-mediated genetic correction, and productive hepatitis C virus infection

Fig. 6

iHeps support the complete HCV infection cycle at levels comparable to PHHs but viral production is decreased in FH-iHeps. a Comparison of control (Ctl) iHeps to PHHs for HCV genome replication (intracellular levels of negative-strand HCV RNA) and for production of total (viral load) and infectious (infectious titer) virus. b Comparison of Ctl iHeps, FH-iHeps and corr-FH-iHeps for HCV genome replication. Cells were cultured in the presence of 500 nM of the HCV polymerase inhibitor sofosbuvir (replication inhibitor) or dimethylsulfoxide as carrier control (DMSO vehicle). c Comparison of Ctl iHeps, FH-iHeps and corr-FH-iHeps for production of total and infectious virus. Results are expressed as mean ± SEM (n = 4 to 7 experiments). Statistical significance was determined by Student’s test, *P < 0.05, **P < 0.01, ***P < 0.001

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Stem Cell Research & Therapy

ISSN: 1757-6512