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Mesenchymal stem cells: current clinical progress in ARDS and COVID-19
Stem Cell Research & Therapy volume 11, Article number: 305 (2020)
Acute respiratory distress syndrome (ARDS) develops rapidly and has a high mortality rate. Survivors usually have low quality of life. Current clinical management strategies are respiratory support and restricted fluid input, and there is no suggested pharmacological treatment. Mesenchymal stromal cells (MSCs) have been reported to be promising treatments for lung diseases. MSCs have been shown to have a number of protective effects in some animal models of ARDS by releasing soluble, biologically active factors. In this review, we will focus on clinical progress in the use of MSCs as a cell therapy for ARDS, which may have clinical implications during the coronavirus disease 2019 (COVID-19) pandemic.
Acute respiratory distress syndrome (ARDS) refers to the diffuse damage to the pulmonary capillary endothelium and alveolar epithelium caused by infection, mechanical stimulation, shock, blood transfusion, and other factors and is the main cause of poor prognosis in critically ill patients . The pathological changes associated with ARDS are mainly alveolar-capillary membrane damage, increased permeability leading to inflammatory exudate in the alveoli, epithelial and interstitial edema, pulmonary edema, carbon dioxide diffusion disorders, and gas exchange disorders. This pathology leads to intractable hypoxemia, which eventually leads to respiratory distress . A recent worldwide outbreak of pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ranges from asymptomatic or mild upper respiratory tract infection to severe pneumonia, ARDS, or even death. The possible mechanism is shown in Fig. 1. The nasal epithelium was found to be the first site of infection and transmission among individuals with symptomatic and asymptomatic SARS-CoV-2 infections . Similar to SARS-CoV, the S protein of SARS-CoV-2 binds to angiotensin-converting enzyme 2 (ACE2) and enters cells in a manner catalyzed by transmembrane protease serine 2 (TMPRSS2) protease in type II alveolar epithelial cells [4, 5]. The affinity of the S protein of SARS-CoV-2 for ACE2 is the main determinant of the replication rate of SARS-CoV-2 and the severity of COVID-19 disease . Despite significant advances in supportive treatment techniques such as mechanical ventilation, the incidence and mortality of ARDS remain high. An observational study in Jinyintan Hospital in Wuhan, China, found that a majority (67–85%) of critically ill patients with SARS-CoV-2 infections developed ARDS and that the mortality was as high as 61.5% in patients with ARDS [7, 8]. Thus, effective control and treatment of ARDS are major challenges that all medical units are facing to suppress the mortality ratio of COVID-19 patients. However, to the best of our knowledge, there are still no approved medicines for ARDS; thus, the development of effective treatment strategies or agents is highly desired. Among these, the administration of MSCs is considered to be a promising approach for the treatment of ARDS in different respiratory viral infection models. This review discusses in detail the current laboratory and clinical research progress on the use of MSCs in the treatment of ARDS caused by SARS-CoV-2 and other respiratory infectious viruses and aims to provide a comprehensive view to facilitate future development of treatments in the battle against COVID-19.
MSCs are considered to have broad clinical application prospects, including as a treatment for ARDS. MSCs have broad bioactivities, including repair, immunomodulation, increased alveolar fluid clearance, and regulation of pulmonary vascular endothelial permeability . Although MSCs have a wide range of sources, such as the bone marrow, umbilical cord, fat, amniotic membrane, menstrual blood, and other tissues , MSCs from different sources have significant similarities, such as being adherent and teardrop- or spindle-shaped . MSCs have the advantages of self-renewal, multidirectional differentiation, and immunosuppression. These cells differentiate into osteocytes, chondrocytes, and adipocytes in the appropriate induction medium . Various soluble factors secreted by MSCs have been shown to be related to immune regulation . Extracellular vesicles, including exosomes, have shown strong abilities to repair, regenerate, and protect against various organ injuries [15, 16], which may play important roles in the treatment of ARDS. In addition, due to the low homing rate of MSCs after infusion, the migration of MSCs to the damaged site is also the focus of current research . As a possible mechanism, CD90 binding to the specific integrins b3 and b5 can promote MSC homing. Activation of integrin b1 or b5 has previously been shown in lung injury models [18, 19]. Studies have reported using transgenic MSCs to improve the homing effect , pretreating MSCs with hypoxia , activating the FAK/ERK pathway , and using nanotechnology to build carriers , and so on.
MSCs in COVID-19
The team from Wuhan Central Hospital analyzed the data of 109 patients diagnosed with ARDS and showed that ARDS progressed faster than other respiratory illnesses and that treatment was difficult. In general, the mortality rate of COVID-19 patients with ARDS was high. When the ARDS level became moderate and severe, the mortality rate climbed as high as 70% . Because stem cells are resistant to tissue damage, promote tissue repair, and have immunoregulatory effects, research by scientists worldwide in the field of stem cells is bringing hope for a treatment for respiratory virus-induced pneumonia . In the H9N2-infected mouse model, MSC treatment increased the survival rate and decreased lung edema and histological injury compared to those of the placebo group. MSCs improved gas exchange and reduced the levels of BALF chemokines and cytokines, including GM-CSF, MIG, IL-1α, IFN-γ, IL-6, and TNF-α . Additionally, MSCs were also reported to be effective in treating an H1N1-infected pig model, and the MSC-treated group had decreased viral shedding in nasal swabs and reduced viral replication in the lungs. The virus-induced production of proinflammatory cytokines, including TNF-α and CXCL-10, was inhibited after MSC treatment . Considering the positive results in various respiratory virus-induced pneumonia cases, MSCs will probably also by effective against SARS-CoV-2 virus, especially by reducing the risk of cytokine storms, which cause ARDS and organ failure in patients with severe disease.
At present, over 20 clinical trials of MSCs in treating COVID-19 are in progress, including clinical trials based on MSC derivatives. In a clinical study involving seven COVID-19 pneumonia patients, including elderly patients, in Beijing Youan Hospital, China, MSC transplantation apparently improved the outcomes of all patients by 14 days after MSC injection without adverse effects. Patient pulmonary function and symptoms improved only 2 days after MSC injection. Cytokine-secreting immune cells, including CXCR3+CD4+ T cells, CXCR3+CD8+ T cells, and CXCR3+ NK cells, disappeared within 1 week. The level of the proinflammatory cytokine TNF-α was significantly decreased, which indicated the great potential of MSCs in treating patients with severe ARDS .
In China, on January 27, the First Affiliated Hospital of Zhejiang University Medical College announced the use of stem cells to treat patients with severe disease. In addition, there are currently 9 registered clinical studies of mesenchymal stem cells for the treatment of acute lung injury (ALI) or ARDS. The advantages and potential of mesenchymal stem cells in defending against severe COVID-19 pneumonia-induced ALI or ARDS have been confirmed . Another study found that after infection with COVID-19, MSC treatment inhibited the overactivation of the immune system and promoted endogenous repair by improving the lung microenvironment. However, studies in a larger patient population are needed to further validate MSC therapeutic interventions .
Mechanisms of the therapeutic benefits of MSCs in ARDS
Although many efforts have been made to understand the therapeutic role of MSCs in ARDS, the mechanism of action has not yet been fully elucidated. Investigating the molecular mechanism of MSCs in the treatment of ARDS is of great importance for MSC-based cell therapy. Many preclinical studies have confirmed the therapeutic effect of MSCs in ARDS or ALI (Table S1). MSCs were initially thought to form niches for the propagation of hematopoietic stem cells that were extremely useful in confluent cultures. Initial research has also focused on the capability of MSCs to differentiate in vivo and transform into osteoblasts, chondrocytes, adipocytes, and even myoblasts. Therefore, MSCs can engraft at the site of tissue injury to participate in repairing the damage. Although refined research techniques have been applied, the effectiveness of MSC engraftment is still not satisfactory. The engraftment rates in lung injury models were low (< 1%) [31, 32]. Thus, studies have focused on the ability of MSCs to secrete paracrine factors such as immunoregulatory factors, angiogenic factors, antiapoptotic factors, and cell migration factors. These cytokines promote MSC migration and homing to damaged sites for repair. Other pathways have also been investigated, and it has been shown that MSCs interact with host tissue, including through mitochondrial transfer and direct interactions between cells. The beneficial effects of MSCs are mainly dependent on paracrine mechanisms . Here, we described the potential therapeutic mechanisms of MSCs in ARDS (Fig. 2).
In patients with COVID-19, SARS-CoV-2 infects type II alveolar epithelial cells or other target cells that express ACE2. Chemokines secreted by these cells cause the influx of neutrophils, monocytes, and T cells. The accumulation of inflammatory cells in turn leads to the production of a large number of proinflammatory cytokines, known as a cytokine storm. Cytokine storms are the main cause of ARDS in COVID-19 patients . Cytokine production was observed in many critically ill patients with COVID-19 [8, 35]. The key role of MSC treatment in COVID-19 is anti-inflammatory . MSCs can reduce the production of proinflammatory cytokines, which may alleviate the cytokine storm induced by SARS-CoV-2, and increase the production of IL-10, which can reduce the inflow and aggregation of neutrophils in the lung and reduce the production of TNF-α . KGF secreted by MSCs can reduce injury and promote proliferation and repair of alveolar epithelial cells by increasing surface-active substances, matrix metalloprotein (MMP)-9, IL-1Ra, GM-CSF, etc. . According to the latest report, lung endothelial cells, as a therapeutic target in COVID-19, play important roles in the course of SARS-CoV-2 infection . MSCs can release VEGF and HGF, which work together to stabilize endothelial barrier function by restoring pulmonary capillary permeability. By inhibiting pulmonary vascular endothelial cell apoptosis, enhancing the recovery of VE-cadherin, and reducing proinflammatory factors, MSCs control inflammation and protect the lung endothelial barrier . Since SARS-CoV-2 mainly affects the lungs , the distribution of MSCs in the peripheral blood is mainly concentrated in the lungs after intravenous infusion , indicating that MSCs are a promising treatment option for patients with COVID-19 pneumonia.
Clinical research progress
Currently, only a few reports have detailed the response of patients with acute respiratory distress syndrome to MSCs (Table S2). There have been some new findings in clinical trials completed in the last 5 years. Zheng et al.’s study (NCT01902082) was one of the earliest studies to detect the safety of mesenchymal stem cells in patients with ARDS. In this phase I, placebo-controlled study, no adverse reactions or serious adverse events were reported related to the infusion of MSCs between the two groups, and the length of hospital stay, days without the ventilator, and days without ICU care on the 28th day after treatment were similar. The serum SP-D level in the MSC group on the 5th day was significantly lower than that on day 0 (p = 0.027), and the IL-8 level was not significantly changed. The IL-6 level on day 5 showed a downward trend compared with that of day 0, but it was not statistically significant (p = 0.06). Therefore, the group concluded that allogeneic adipose-derived mesenchymal stem cells are safe for ARDS treatment. However, the clinical effects of using MSC doses are weak, and this strategy needs to be further optimized to achieve the satisfactory ARDS treatment .
In the RUMCESS trial (NCT01849237), the patients were randomly assigned (1:1) to receive either conventional therapy (CT) for septic shock (SS) or CT plus MSCs at a dose of 106/kg intravenously within the first 10 h after SS onset. Except for one patient with myelodysplastic syndrome, all patients developed neutropenia after chemotherapy. Most of the positive blood cultures from patients were gram-negative. The APACHE II baseline scores were 34.2 and 32.2, and the sequential organ failure assessment (SOFA) scores were similar in both groups. After 3 months, 5 of the 8 patients in the CT + MSCs group who survived for 28 days died of sepsis-related organ dysfunction. As a result, MSC administration within the first few hours of SS may increase the short-term survival of patients with neutropenia but cannot prevent death from sepsis-related organ dysfunction over time .
The STem cells for ARDS Treatment (START) trial was a multicenter, open-label, dose-escalation, phase 1 clinical trial. In 2015, Wilson et al. published the results of their START trial (NCT01775774). In nine patients, there were no prespecified infusion-related events or treatment-related adverse events. Severe adverse events in three patients were subsequently noted several weeks after infusion but were thought to occur before MSC injection based on the MRI results. Nine patients with moderate-to-severe ARDS tolerated a single intravenous infusion of human BM-MSC derived from allogeneic bone marrow well. The authors believe that more patients are needed to thoroughly investigate the response of ARDS patients to MSCs .
In a follow-up study, Simonson et al. (NCT02097641) performed a detailed analysis of the immunomodulatory and proteomics characteristics of two patients with severe refractory ARDS. Two patients received 2 × 106 cells per kilogram, and patients showed improvements in breathing, hemodynamics, and multiple organ failure. At the same time, a variety of lung and systemic inflammatory markers, including epithelial cell apoptosis, leakage of alveolar-capillary fluid, proinflammatory cytokines, microRNAs, and chemokines, also declined. The results of this study also suggest that the use of adoptively transplanted mesenchymal stem cells for lung protection in patients with ARDS is beneficial but has not been validated. Further research is needed .
Matthay et al. conducted a prospective, double-blind, multicenter, randomized phase 2a trial (NCT02097641) to assess the safety of MSCs in patients with moderate-to-severe ARDS. This group recruited ventilation patients with moderate-to-severe ARDS (partial oxygen pressure to fractional inhaled oxygen ratio < 27 kPa, positive end-expiratory pressure [PEEP] 8 cmH2O) at five university medical centers. There was no difference in the 28-day mortality between the MSC group and the placebo group. The results show that for patients with moderate-to-severe ARDS, a single intravenous dose of MSCs is safe . Based on this result, Zhang and colleagues further studied the rationale for why the MSC-administered group exhibited an increased 28-day mortality (not significant). Their research demonstrated that MSC treatment might be beneficial or detrimental depending on the patient’s specific pulmonary microenvironment, which included the levels of IL-6 and fibronectin, and total antioxidant capacity (TAC). MSC treatment is protective in a mouse model with reduced concentrations of IL-6 and fibronectin and increased levels of TAC, while MSCs worsened injury in the opposite conditions. This result provided an important rationale for a precision medicine approach for MSC treatment .
ARDS is a severe acute respiratory failure syndrome with a high mortality rate. Despite extensive research, there is currently no specific support for the treatment of ARDS. Because MSCs have obvious therapeutic effects, such as promoting the repair of epithelial and endothelial tissues, clearance of alveolar fluid and microorganisms, and anti-inflammatory and antiapoptotic effects, MSC-based cell therapy is a promising strategy for the treatment of ARDS. The safety and possible efficacy have been demonstrated in some ARDS patients. Limited clinical data have shown that systemic administration of MSCs can significantly alleviate lung injury in COVID-19 patients. With the development of research involving MSCs for the treatment of COVID-19, MSC therapy may be a strategy for responding to the current epidemic. The mechanism of action of MSCs is complex. The key mechanisms include paracrine mechanisms, extracellular vesicles, or direct contact of cells with metastatic cell contents. Although some progress has been made, there is not enough clinical evidence to prove the effectiveness of MSCs in the treatment of ARDS. The survival rate of the cells, homing efficiency, gene mutation after transplantation, and tumorigenicity are the current challenges regarding clinical applications. Therefore, large-scale, long-term, multicenter trials are needed to further explore the therapeutic effects and safety of MSCs.
Acute respiratory distress syndrome
Mesenchymal stromal cells
Coronavirus disease 2019
Severe acute respiratory syndrome coronavirus 2
Angiotensin-converting enzyme 2
Acute lung injury
STem cells for ARDS Treatment
Total antioxidant capacity
Transmembrane protease serine 2
Granulocyte-macrophage colony-stimulating factor
Tumor necrosis factor
Neutrophil extracellular traps
Regulatory T cell
Alveolar type II
Reactive oxygen species
Fibroblast growth factor 7
Keratinocyte growth factor
Vascular endothelial growth factor
Hepatocyte growth factor
Sequential organ failure assessment
Michael AM, Zemans RL, Zimmerman GA, et al. Acute respiratory distress syndrome. Nat Rev Dis Primers. 2019;5:18. https://doi.org/10.1038/s41572-019-0069-0.
Thompson BT, Chambers RC, Liu KD. Acute respiratory distress syndrome. N Engl J Med. 2017;377:562–72. https://doi.org/10.1056/NEJMra1608077.
Sungnak W, Huang N, Bécavin C, et al. SARS-CoV-2 entry factors are highly expressed in nasal epithelial cells together with innate immune genes. Nat Med. 2020. https://doi.org/10.1038/s41591-020-0868-6.
Li W, Moore MJ, Vasilieva N, et al. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 2003;426:450–4. https://doi.org/10.1038/nature02145.
Matsuyama S, Nagata N, Shirato K, et al. Efficient activation of the severe acute respiratory syndrome coronavirus spike protein by the transmembrane protease TMPRSS2. J Virol. 2010;84:12658–64. https://doi.org/10.1128/JVI.01542-10.
Hoffmann M, Kleine-Weber H, Schroeder S, et al. SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor. Cell. 2020;181:271–280.e8. https://doi.org/10.1016/j.cell.2020.02.052.
Rothlin RP, Vetulli HM, Duarte M, et al. Telmisartan as tentative angiotensin receptor blocker therapeutic for COVID-19. Drug Dev Res. 2020;10:1002/ddr.21679. https://doi.org/10.1002/ddr.21679.
Huang C, Wang Y, Li X, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395:497–506. https://doi.org/10.1016/S0140-6736(20)30183-5.
Yang X, Yu Y, Xu J, et al. Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study. Lancet Respir Med. 2020;8:e26. https://doi.org/10.1016/S2213-2600(20)30079-5.
Walter J, Ware LB, Matthay MA. Mesenchymal stem cells: mechanisms of potential therapeutic benefit in ARDS and sepsis. Lancet Respir Med. 2014;2:1016–26. https://doi.org/10.1016/S2213-2600(14)70217-6.
Main H, Munsie M, O’Connor MD. Managing the potential and pitfalls during clinical translation of emerging stem cell therapies. Clin Transl Med. 2014;3:10. https://doi.org/10.1186/2001-1326-3-10.
Javazon E, Colter D, Schwarz E, et al. Rat marrow stromal cells are more sensitive to plating density and expand more rapidly from single-cell-derived colonies than human marrow stromal cells. Stem Cells. 2010;19:219–25. https://doi.org/10.1634/stemcells.19-3-219.
Pittenger M, Mackay A, Beck S, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143–7. https://doi.org/10.1126/science.284.5411.143.
Fan XL, Zhang Y, Li X, et al. Mechanisms underlying the protective effects of mesenchymal stem cell-based therapy. Cell Mol Life Sci. 2020:1–24. https://doi.org/10.1007/s00018-020-03454-6.
Haider KH, Aramini B. Mircrining the injured heart with stem cell-derived exosomes: an emerging strategy of cell-free therapy. Stem Cell Res Ther. 2020;11:23. https://doi.org/10.1186/s13287-019-1548-7.
Zou X, Gu D, Xing X, et al. Human mesenchymal stromal cell-derived extracellular vesicles alleviate renal ischemic reperfusion injury and enhance angiogenesis in rats. Am J Transl Res. 2016;8:4289–99.
Ullah M, Liu DD, Thakor AS. Mesenchymal stromal cell homing: mechanisms and strategies for improvement. iScience. 2019;15:421–38. https://doi.org/10.1016/j.isci.2019.05.004.
Hakanpaa L, Kiss EA, Jacquemet G, et al. Targeting β1-integrin inhibits vascular leakage in endotoxemia. Proc Natl Acad Sci U S A. 2018;115:E6467–76. https://doi.org/10.1073/pnas.1722317115.
Ganter MT, Roux J, Miyazawa B, et al. Interleukin-1β causes acute lung injury via αvβ5 and αvβ6 integrin-dependent mechanisms. Circ Res. 2008;102:804–12. https://doi.org/10.1161/CIRCRESAHA.107.161067..
Han J, Liu Y, Liu H, et al. Genetically modified mesenchymal stem cell therapy for acute respiratory distress syndrome. Stem Cell Res Ther. 2019;10:386. https://doi.org/10.1186/s13287-019-1518-0.
Meng SS, Xu XP, Chang W, et al. LincRNA-p21 promotes mesenchymal stem cell migration capacity and survival through hypoxic preconditioning. Stem Cell Res Ther. 2018;9:280. https://doi.org/10.1186/s13287-018-1031-x.
Lu X, Han J, Xu X, et al. PGE2 promotes the migration of mesenchymal stem cells through the activation of FAK and ERK1/2 pathway. Stem Cells Int. 2017;2017:8178643. https://doi.org/10.1155/2017/8178643.
Thanuja MY, Anupama C, Ranganath SH. Bioengineered cellular and cell membrane-derived vehicles for actively targeted drug delivery: so near and yet so far. Adv Drug Deliv Rev. 2018;132:57–80. https://doi.org/10.1016/j.addr.2018.06.012.
Liu Y, Sun W, Li J, et al. Clinical features and progression of acute respiratory distress syndrome in coronavirus disease. medRxiv. 2020. https://doi.org/10.1101/2020.02.17.20024166.
Khoury M, Cuenca J, Cruz FF, et al. Current status of cell-based therapies for respiratory virus infections: applicability to COVID-19. Eur Respir J. 2020. https://doi.org/10.1183/13993003.00858-2020.
Li Y, Xu J, Shi W, et al. Mesenchymal stromal cell treatment prevents H9N2 avian influenza virus-induced acute lung injury in mice. Stem Cell Res Ther. 2016;7:159. https://doi.org/10.1186/s13287-016-0395-z.
Khatri M, Richardson LA, Meulia T. Mesenchymal stem cell-derived extracellular vesicles attenuate influenza virus-induced acute lung injury in a pig model. Stem Cell Res Ther. 2018;9:17. https://doi.org/10.1186/s13287-018-0774-8.
Leng Z, Zhu R, Hou W, et al. Transplantation of ACE2-mesenchymal stem cells improves the outcome of patients with COVID-19 pneumonia. Aging Dis. 2020;11:216–28. https://doi.org/10.14336/AD.2020.0228.
Atluri S, Manchikanti L, Hirsch JA. Expanded umbilical cord mesenchymal stem cells (UC-MSCs) as a therapeutic strategy in managing critically ill COVID-19 patients: the case for compassionate use. Pain Physician. 2020;23:E71–83.
Shetty AK. Mesenchymal stem cell infusion shows promise for combating coronavirus (COVID-19)-induced pneumonia. Aging Dis. 2020;11:462–4. https://doi.org/10.14336/AD.2020.0301.
Rojas M, Xu J, Woods CR, et al. Bone marrow-derived mesenchymal stem cells in repair of the injured lung. Am J Respir Cell Mol Biol. 2005;33:145–52. https://doi.org/10.1165/rcmb.2004-0330OC.
Loi R, Beckett T, Goncz KK, et al. Limited restoration of cystic fibrosis lung epithelium in vivo with adult bone marrow-derived cells. Am J Respir Crit Care Med. 2006;173:171–9. https://doi.org/10.1164/rccm.200502-309OC.
Antebi B, Mohammadipoor A, Batchinsky AI, et al. The promise of mesenchymal stem cell therapy for acute respiratory distress syndrome. J Trauma Acute Care Surg. 2018;84:183–91. https://doi.org/10.1097/TA.0000000000001713.
Taghavi-farahabadi M, Mahmoudi M, Soudi S, et al. Hypothesis for the management and treatment of the COVID-19-induced acute respiratory distress syndrome and lung injury using mesenchymal stem cell-derived exosomes. Med Hypotheses. 2020. https://doi.org/10.1016/j.mehy.2020.109865.
Mehta P, McAuley DF, Brown M, et al. COVID-19: consider cytokine storm syndromes and immunosuppression. Lancet. 2020;395:1033–4. https://doi.org/10.1016/S0140-6736(20)30628-0.
Liu S, Peng D, Qiu H, et al. Mesenchymal stem cells as a potential therapy for COVID-19. Stem Cell Res Ther. 2020;11:169. https://doi.org/10.1186/s13287-020-01678-8.
Mei SH, McCarter SD, Deng Y, et al. Prevention of LPS induced acute lung injury in mice by mesenchymal stem cells overexpressing angiopoietin 1. PLoS Med. 2007;4:e269. https://doi.org/10.1371/journal.pmed.0040269.
Shyamsundar M, McAuley DF, Ingram RJ, et al. Keratinocyte growth factor promotes epithelial survival and resolution in a human model of lung injury. Am J Respir Crit Care Med. 2014;189:1520–9. https://doi.org/10.1164/rccm.201310-1892OC.
Teuwen LA, Geldhof V, Pasut A, et al. COVID-19: the vasculature unleashed. Nat Rev Immunol. 2020:1–3. https://doi.org/10.1038/s41577-020-0343-0Guzik.
Yang Y, Chen QH, Liu AR, et al. Synergism of MSC-secreted HGF and VEGF in stabilising endothelial barrier function upon lipopolysaccharide stimulation via the Rac1 pathway. Stem Cell Res Ther. 2015;6:250. https://doi.org/10.1186/s13287-015-0257-0.
Guzik TJ, Mohiddin SA, Dimarco A, et al. COVID-19 and the cardiovascular system: implications for risk assessment, diagnosis, and treatment options. Cardiovasc Res. 2020. https://doi.org/10.1093/cvr/cvaa106.
Fischer UM, Harting MT, Jimenez F, et al. Pulmonary passage is a major obstacle for intravenous stem cell delivery: the pulmonary first-pass effect. Stem Cells Dev. 2009;18:683–92. https://doi.org/10.1089/scd.2008.0253.
Zheng G, Huang L, Tong H, et al. Treatment of acute respiratory distress syndrome with allogeneic adipose-derived mesenchymal stem cells: a randomized, placebo-controlled pilot study. Respir Res. 2014;15:39. https://doi.org/10.1186/1465-9921-15-39.
Galstian GM, Parovichnikova EN, Makarova PM, et al. The results of the Russian clinical trial of mesenchymal stromal cells (MSCs) in severe neutropenic patients (pts) with septic shock (SS) (RUMCESS trial). Blood. 2015;126:2220.
Wilson JG, Liu KD, Zhuo H, et al. Mesenchymal stem (stromal) cells for treatment of ARDS: a phase 1 clinical trial. Lancet Respir Med. 2015;3:24–32. https://doi.org/10.1016/S2213-2600(14)70291-7.
Simonson OE, Mougiakakos D, Heldring N, et al. In vivo effects of mesenchymal stromal cells in two patients with severe acute respiratory distress syndrome. Stem Cells Transl Med. 2015;4:1199–213. https://doi.org/10.5966/sctm.2015-0021.
Matthay MA, Calfee CS, Zhuo H, et al. Treatment with allogeneic mesenchymal stromal cells for moderate to severe acute respiratory distress syndrome (STARS study): a randomised phase 2a safety trial. Lancet Respir Med. 2019;7:154–62. https://doi.org/10.1016/S2213-2600(18)30418-1.
Zhang H, Li Y, Slutsky AS. Precision medicine for cell therapy in acute respiratory distress syndrome. Lancet Respir Med. 2019;7:e13. https://doi.org/10.1016/S2213-2600(19)30089-X.
This work was supported by grants from the Clinical Research Support Fund of PLA General Hospital (2018FC-WJFWZX-1-03) and from the Military Medical Technologies Young Scientist Cultivate Project (20QNPY099).
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: Table S1. Summary of the therapeutic benefits of MSCs in current preclinical models of experimental ARDS/ALI. Table S2. Ongoing and new studies of mesenchymal stem cell-based therapies for COVID-19 pneumonia.
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Xiao, K., Hou, F., Huang, X. et al. Mesenchymal stem cells: current clinical progress in ARDS and COVID-19. Stem Cell Res Ther 11, 305 (2020). https://doi.org/10.1186/s13287-020-01804-6
- Acute respiratory distress syndrome
- Cell therapy
- Mesenchymal stem cells
- Clinical trials