Fig. 1

Schematic representation of the hiPSC differentiation protocol for multizone ocular progenitor cells and multiocular organoids. A, B Schemes of the differentiation protocol for the 2D generation of multizone ocular progenitor cells (mzOPCs) consisting of neuroectoderm, surface ectoderm and neural crest structures. At day 30, mzOPCs were manually picked and developed in suspension into 3D multiocular, retinal, corneal, and RPE organoids. Abbreviations: ATRA, all-trans retinoic acid; bFGF, basic fibroblast growth factor; DIV, days in vitro; DKK1, Dickkopf WNT Signaling Pathway Inhibitor 1; hiPSC, human induced pluripotent stem cells; IGF1, insulin-like growth factor 1; T3, triiodothyronine; RPE, retinal pigment epithelium