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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Emilin-2 is a component of bone marrow extracellular matrix regulating mesenchymal stem cell differentiation and hematopoietic progenitors

Fig. 5

Frequency of in vivo hematopoietic progenitors in adult wild-type and Emilin-2 null mice. BM were isolated from wild-type and Emilin2/ mice of 3, 6 and 10 months of age and subjected to flow cytometry with different antibodies. A Representative flow cytometry analysis of hematopoietic progenitors. Sca1/c-Kit+ events (plot iii of Fig. 4A) were subjected to further analysis, based on CD16/32 and CD34 signals, to identify GMP (CD16/32+/CD34+; top square), CMP (CD16/32/CD34+; middle square) and MEP (CD16/32/CD34; bottom square). B Percentages of CMP, GMP and MEP in Lin subpopulation and percentages of Lin cells on total live cells in BM of 3-month-old (wild type, n = 4; Emilin2/, n = 4). C Percentages of CMP, GMP and MEP in Lin subpopulation and percentages of Lin cells on total live cells in BM of 6-month-old (wild-type, n = 10; Emilin2/, n = 6) and 10-month-old (wild type, n = 5–7; Emilin2/, n = 5–9) mice. WT, wild-type. See also Additional file 1: Fig. S4

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