Fig. 3

SNHG1 acts as a ceRNA for miR-328-3p. A The transfection efficacy of miR-328-3p mimics and inhibitor was verified by qRT-PCR. B The expression of SNHG1 was regulated by miR-328-3p. C SNHG1 can also govern the expression of miR-328-3p. D The dual-luciferase reporter assay was conducted in 293 T cells. The luciferase activities of the wild-type or Mutant SNHG1 reporter gene were detected after 24 h of co-transfection with mimics NC or miR-328-3p mimics. The light intensity from firefly luciferase is normalized by Renilla luciferase. E The potential binding sites of SNHG1 and miR-328-3p predicted by the biological prediction software and the site changes in mutant SNHG1. *P < 0.05, **P < 0.01, ***P < 0.001