Fig. 4

MiR-328-3p effects the odontogenic differentiation of hDPSCs. A-D After cell transfection of miR-328-3p mimics and inhibitor, relative ALP, DMP-1 and DSPP expression and ALP, DMP-1 and DSPP expression were measured by qRT-PCR and Western blot on the 5th day of mineralization induction. E, F On the 7th day of mineralization induction, ALP staining and activity were performed to determine the odontogenic differentiation of hDPSCs. Similarly, Alizarin Red staining and quantification were performed on the 14th day. Scale bar = 100 μm. G Immunofluorescence Staining showed different expression levels of DMP-1 and DSPP in transfected hDPSCs. Scale bar = 20 μm. *P < 0.05, **P < 0.01, ***P < 0.001