Fig. 6

SNHG1 regulates Wnt/β-catenin pathway via competitively binding miR-328-3p in the odontogenic differentiation of hDPSCs. A-D After miR-328-3p was overexpressed, Axin1 was down-regulated at the gene and protein level, thereby promoting Wnt/β-catenin pathway, which manifests as an up-regulation of (active) β-catenin. After miR-328-3p was silent, there were opposite effects. E–H SNHG1 had opposite effects on Axin1, Axin1 and (active) β-catenin expression, compared with miR-328-3p. I Western blot analysis indicated that the miR-328-3p mimics rescued the SNHG1 mediated up-regulation of Axin1 and (active) β-catenin. J qPCR validated western blot results of Axin1 at the gene level. *P < 0.05, **P < 0.01, ***P < 0.001