Fig. 1

Late passage MSC(AT) are enriched in senescence markers. A Size (FSC-A), B granularity (SSC-A), C autofluorescence, D doubling time (days), E representative example of β-galactosidase staining (SA-β-Gal), and F summary data for percent of SA-β-Gal in early passage (EP) and late passage (LP) MSC(AT). Graphs A–D and F summarize data of 6 adult and 4 pediatric MSC(AT) samples at EP (p4.1 ± 0.6) and LP (p21.0 ± 6.0). Data shown as mean ± SD, comparisons done using paired Wilcoxon test, *p < 0.05, **p < 0.01. Scale bars in E represent 200 μm