Table 1 Culture medium and exogenous factors supplied during iPSCs differentiation into iMphs
Protocol | Stage | Exogenous differentiation factors | Medium |
|---|---|---|---|
EB-S | PSC expansion | FGF2 | KO-DMEM |
DMEM/F12 | |||
AdvDMEM | |||
Stage 1 | NA | KO-DMEM | |
DMEM/F12 | |||
AdvDMEM | |||
Stage 2 and 3 | IL3 + M-CSF | X-VIVO 15 | |
Stage 4 | M-CSF | X-VIVO 15 RPMI | |
EB-F HP + MY | PSC expansion | FGF2 | mTeSR1 |
DMEM/F12 | |||
Stage 1 | BMP4/VEGF/SCF | mTeSR1 | |
DMEM/F12 | |||
Stage 2 and 3 | IL3 + M-CSF | X-VIVO 15 | |
Stage 4 | M-CSF | X-VIVO 15 + RPMI | |
EB-F HP → MY | PSC expansion | FGF2 | DMEM/F12 |
AdvDMEM | |||
Stage 1 | BMP4/VEGF/SCF/ FGF2/Flt3L/TPO/CHIR/ActA | StemPro-34 | |
mTeSR1 | |||
(KO-DMEM) | |||
Stage 2 | VEGF/SCF/ FGF2/Flt3L/TPO/IL3/M-CSF | StemPro-34 | |
Stage 3 | VEGF/SCF/ FGF2/Flt3L/IL3/IL6M-CSF/GM-CS | StemPro-34 | |
RPMI | |||
Stage 4 | M-CSF | RPMI | |
2D-F | PSC expansion | NA | mTeSR1 |
E8 | |||
TeSR-E8 | |||
Stage 1 | BMP4/VEGF/ FGF2/ CHIR/ActA/S B/SCF | StemPro-34 | |
mTeSR1 | |||
E8 | |||
IMDM/F12 | |||
Stage 2 | VEGF/ FGF2/SCF/TPO/Flt3 L/IL3/IL6 | StemPro-34 | |
IMDM/F12 | |||
(DMEM/F12) | |||
Stage 3 | FGF2/SCF/Flt3 L/IL3/IL6/ MCSF | StemPro-34 | |
IMDM/F12 | |||
(DMEM/F12) | |||
Stage 4 | M-CSF | IMDM/F12 | |
RPMI |