Table 1 Regulation of miRNAs in osteogenic differentiation of C3H10T1/2
From: Regulatory of miRNAs in tri-lineage differentiation of C3H10T1/2
miRNA | Expression | Target | Expression of target | Distribution | Function | Experiment | Animal model | References |
|---|---|---|---|---|---|---|---|---|
miR-335-5p | ↑ | DKK1 | ↓ | Cytoplasm | miR-335-5p stimulates Wnt signal transmission and facilitates osteogenetic differentiative activity via the down-regulation of DKK1 | In vitro | – | [47] |
miR-129-5p | ↑ | DKK3 | ↓ | Cytoplasm | miR-129-5p promotes the osteogenetic differentiative activity of BMSCs via inhibiting the expressing of DKK3, which interacts with β-catenin directly | In vitro and in vivo | Mice/36 | [48] |
miR-21a | ↑ | Smad7 | ↓ | Nucleus | Phytol-treatment promotes osteoblast differentiation via highly expressed Runx2 due to down-regulation of Smad7 by miR-21a | In vitro | – | [6] |
miR-140-5p | ↑ | Runx2, ALP, Spp1 and Bglap3 | ↑ | Nucleus | miR-140-5p promotes osteogenic differentiation via enhancing the expression of osteoblast differentiation-related genes | In vitro and in vivo | Mice/32 | [43] |
miR-582-5p | ↓ | Runx2 | ↑ | Cytoplasm | miR‐582‐5p negatively regulates osteogenic differentiation by down-regulating the activity of ALP and mRNA/protein levels of ALP and Runx2 | In vitro | – | [49] |
miR-590-5p | ↑ | Smad7 | ↓ | Nucleus | miR-590-5p facilitates the differentiative activity of osteoblasts via straightly targeting Smad7 and non-straightly protecting and stabilizing the Runx2 protein from Smurf2-mediated decomposition | In vitro | – | [50] |
miR-144-3P | ↓ | Smad4 | ↑ | Cytoplasm | miR-144-3p modulates the osteogenetic differentiative and proliferative activity in a negative way of via suppressing the Smad4 protein level | In vitro | – | [51] |
miR-324-5p | ↓ | Gpc1 | ↑ | Cytoplasm | miR-324-5p direct targets Gpc1 and regulates Hedgehog signaling, reducing the content of Ihh and BMP-2-triggered alkaline phosphatase | In vitro | – | [10] |
miR-194 | ↑ | COUP-TFII | ↓ | Cytoplasm | Enforced expressing of miR-194 remarkably reinforces the differentiative activity of osteoblasts, whereas it suppresses the differentiative activity of adipocytes via reducing COUP-TFII mRNA and protein contents | In vitro | – | [52] |
miR-125b | ↓ | Cbfβ | ↑ | Cytoplasm | miR-125b is a negative modulatory factor of osteogenetic differentiative activity via straightly down-regulating Cbfβ and non-straightly acting on Runx2 | In vitro | – | [53] |
miR-30a | ↓ | BMP9 | ↑ | Cytoplasm | miR-30a modulates BMP9-triggered osteogenetic differentiative activity in a negative way | In vitro and in vivo | Mice/44 | [45] |
miR-433 | ↓ | Runx2 | ↑ | Cytoplasm | miR-433 inhibits BMP-2-triggered osteogenetic differentiative activity via reducing the content of Runx2 transcript | In vitro | – | [46] |
miR-223 | ↓ | Fgfr2 | ↑ | Cytoplasm | miR-223 increases FGFR2 protein level and plays an inhibitory role in osteoblast differentiation | In vitro | – | [7] |
miR-30e | ↓ | LRP6 | ↑ | Cytoplasm | miR-30e overexpression negatively affects osteoblast differentiation by targeting LRP6 and the canonical Wnt/β-catenin signal transmission | In vitro | – | [8] |