Fig. 5

Small molecules improve iPSC-CM sarcomeric-related gene expression and polyploidy. The figures represent the combined relative gene expression of all three cell lines evaluated across multiple differentiation batches at D27. Relative sarcomeric gene expression a ACTC1 in CTRL versus AA, GW, and T3, respectively: 0.24 (0.15–1.34) versus 0.16 (0.10 – 0.30; n.s.), 0.43 (0.24–1.02; p = 0.0008), and 1.08 (0.25–6.28; p < 0.0001); b TNNI3 in CTRL versus AA, GW, and T3, respectively: 0.73 (0.37–1.55) versus 0.46 (0.2–0.68; p = 0.012), 1.08 (0.47–1.97; p = 0.002), and 0.63 (0.43–1.45; p = 0.09). c–d DNA content per single nucleus was measured using FACS on three independent experiments with iPSC lines: UKKi036-C, UKKi032-C, and UKKi037-C combined n = 6–12. c) Offset histogram of nuclei. d) DNA content per nuclei in treated iPSC-CMs normalized to CTRL for 2N, > 2N and > 4N. DNA content per nucleus > 4N CTRL 1, AA 2.16, GW 1.48, T3 1.06. p-values for polyploidism calculated via 2-way ANOVA ** = 0.0023, *** = 0.0003. Fig. S5 for more detail