Fig. 1

Human monocytes phagocytose viable and heat-inactivated human MSCs in a dose-dependent manner. a Gating strategy and representative plots of CD14 + monocytes alone (−MSC) or cultured with CBO-stained MSCs (+MSC) or HI-MSCs (+HI-MSC) for 24 h. b The percent of CD14 + monocytes positive for CBO increases with increasing dose of MSCs and HI-MSCs after 24 h of non-adherent co-culture (mean ± SEM, n = 3 independent experiments). c Representative Z-stack cross sections at 1 µm intervals of a CD14-AlexaFluor 488 stained monocyte (green) after 24 h coculture with CBO-stained MSCs (red). Nuclei stained blue with Hoechst33342