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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Efferocytosis of viable versus heat-inactivated MSC induces human monocytes to distinct immunosuppressive phenotypes

Fig. 3

Human T-cell proliferation is suppressed by MSC-licensed monocytes even after residual MSCs are removed. a Schematic of process used to remove residual MSCs after 24 h MSC:PBMC coculture. Residual MSCs were labeled with a CD90 antibody conjugated to PE and then tagged with Anti-PE MojoSort nanobeads and pulled down with a magnet. b Representative plots showing efficiency of MSC removal. Left panels show gate for CD90 + MSCs and the right panels show an overlay of total cells (red) and CD90 + MSCs (light blue). c Quantification of T-cell proliferation as a percent of the positive stim control for 1:5 MSC:PBMC (mean ± SEM, n = 3 independent experiments, *p < 0.05 calculated after 2-way ANOVA with Tukey post hoc test)

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Stem Cell Research & Therapy

ISSN: 1757-6512