Fig. 4

Human monocytes experience differential phenotypic shift upon efferocytosis of viable- versus HI-MSC. a Representative plots of CD14 versus CD16 of CD14 + monocytes after 24 h of efferocytosis of MSCs. Red gate = classical monocytes (CM), Blue gate = intermediate monocytes (IM), and Orange gate = non-classical monocytes (NCM). b Quantification of monocytes identified as CM, IM, or NCM (mean ± SEM, n = 3 independent experiments, *p < 0.05 calculated after 2-way ANOVA with Tukey post hoc test). c CD86 versus CD163 representative plots of CM (red), IM (blue), and NCM (orange) subsets overlaid and associated histograms. d Quantification of CD86 MFI for CM, IM, and NCM. (Mean ± SEM, n = 3 independent experiments, *p < 0.05 calculated after 1-way ANOVA with Tukey post hoc test). e Quantification of CD163 MFI for CM, IM, and NCM. For the gating strategy, refer to Additional file 1: Figure S4. (Mean ± SEM, n = 3 independent experiments, *p < 0.05 calculated after 1-way ANOVA with Tukey post hoc test)