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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Efferocytosis of viable versus heat-inactivated MSC induces human monocytes to distinct immunosuppressive phenotypes

Fig. 5

Efferocytosis of viable but not HI-MSCs by monocytes induces IL-10 secretion and activation of IDO. a Schematic of process used to negatively select monocytes. Non-monocytes were labeled with a cocktail of biotin-conjugated antibodies, then tagged with streptavidin MojoSort nanobeads, and pulled down with a magnet. Quantification of b IL-10 and c kynurenine production detected after 2-day monocyte culture without or with efferocytic-licensing by viable or HI-MSCs. LOD = Limit of Detection (LODIL-10 = 19 pg/mL, LODKyn = 7 µM, LODTNF-α = 15 pg/mL (data not shown))

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Stem Cell Research & Therapy

ISSN: 1757-6512