Efferocytosis of viable versus heat-inactivated MSC induces human monocytes to distinct immunosuppressive phenotypes

Table 1 Product and staining information for on-target, isotype, and blocking antibodies used for experiments

Antibody target	BioLegend Cat#	RRID#	Fluorophore	Isotype RRID#	Conc. (µg/mL)
CD3	317334	AB_2561452	PE-Cy7	AB_2864288	1.25
CD14	325610	AB_830683	Alexa 488	AB_2890263	3.00
CD16	302067	AB_2876587	PE-Fire640	AB_2937018	1.00
CD86	305419	AB_1575070	PerCP-Cy5.5	AB_2937017	0.25
CD90	328110	AB_893433	PE	AB_326435	2.50
CD163	333614	AB_2562641	PE-Cy7	AB_326448	2.00
CD206	321130	AB_2616867	PE-Dazzle594	AB_2923261	3.00

The antibodies listed are individual items and their associated isotypes. Listed staining concentrations are stain-index optimized for the on-target antibodies and were used for the on-target and associated isotype antibodies. Any antibody products that were used as part of a kit are not listed in this table (see “Isolation of Human Monocytes” and “Depletion of Non-Phagocytosed MSCs” sections)

ISSN: 1757-6512