Skip to main content
Download PDF

A first-in-human clinical study of laparoscopic autologous myoblast sheet transplantation to prevent delayed perforation after duodenal endoscopic mucosal dissection

Stem Cell Research & Therapy volume 15, Article number: 117 (2024) Cite this article

Abstract

Background

The detection rate of superficial non-ampullary duodenal epithelial tumors (SNADETs) has recently been increasing. Large tumors may contain malignant lesions and early therapeutic intervention is recommended. Endoscopic mucosal dissection (ESD) is considered a feasible treatment modality, however, the anatomical and physiological characteristics of the duodenum create a risk of postoperative perforation after ESD.

Methods

To explore whether myoblast sheet transplantation could prevent delayed perforation after ESD, a first-in-human (FIH) clinical trial of laparoscopic autologous myoblast sheet transplantation after duodenal ESD was launched. Autologous myoblast sheets fabricated from muscle tissue obtained seven weeks before ESD were transplanted laparoscopically onto the serous side of the ESD. The primary endpoints were the onset of peritonitis due to delayed perforation within three days after surgery and all adverse events during the follow-up period.

Results

Three patients with SNADETs ≥ 20 mm in size underwent transplantation of a myoblast sheet onto the serous side of the duodenum after ESD. In case 1, The patient’s postoperative course was uneventful. Endoscopy and abdominal computed tomography revealed no signs of delayed perforation. Despite incomplete mucosal closure in case 2, and multiple micro perforations during ESD in case 3, cell sheet transplantation could prevent the postoperative massive perforation after ESD, and endoscopy on day 49 after transplantation revealed no stenosis.

Conclusions

This clinical trial showed the safety, efficacy, and procedural operability of this novel regenerative medicine approach involving transplanting an autologous myoblast sheet laparoscopically onto the serosa after ESD in cases with a high risk of delayed perforation. This result indicates the potential application of cell sheet medicine in treating various abdominal organs and conditions with minimal invasiveness in the future.

Trial registration

jRCT, jRCT2073210094. Registered November 8 2021,

https://jrct.niph.go.jp/latest-detail/jRCT2073210094.

Introduction

Owing to its rarity, the duodenum has not received much attention as a site of tumor development. The incidence of superficial non-ampullary duodenal epithelial tumors (SNADETs), in particular, is said to be 0.02-0.5% among autopsy series [1]. However, the rate of detection of these tumors has recently been increasing owing to advances in endoscopic technology and increasing awareness of this disease [2]. Duodenal cancer is estimated to account for 0.5% of all gastrointestinal cancers. A recent Japanese study using a large-scale national database indicated that the incidence of duodenal cancer registered in 2016 was 23.7 per 1,000,000 person-years. The authors described the incidence as increasing worldwide [3,4,5].

Accumulating evidence indicates that even if a tumor is localized to the mucosal layer, large tumors may contain malignant lesions [6], so early therapeutic intervention is recommended for these tumors [4]. The duodenum is located near vital organs, such as the pancreas and bile duct, and invasive procedures, such as Whipple operation, are needed to treat cases of duodenal cancer. In Japan, due to widespread esophagogastroduodenoscopy being performed to screen for gastric cancer, more than 50% of duodenal cancer cases are diagnosed at the localized stage, and endoscopic resection of these superficial tumors is recommended. According to an analysis of a national cancer registry conducted by Yoshida et al., approximately 48% of these cancers were treated by endoscopic resection, with favorable short- and long-term outcomes achieved [3].

Endoscopic resection, such as endoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD), are considered feasible treatment modalities for these tumors. In contrast to the widespread application of ESD for early gastric and colorectal cancer, ESD for duodenal tumors has been hampered by the fact that maneuvering the scope is difficult because of the narrow curling lumen and very thin wall of the duodenum. Furthermore, mucosal defects after ESD are exposed to irritant digestive contents, such as bile and pancreatic juices, which impair the integrity of the remnant duodenal wall. These anatomical and physiological characteristics create a risk of postoperative perforation after ESD, which is reported in 0-14% of cases [7] and if delayed perforation developed emergency surgery is often required to cure lethal peritonitis [8,9,10]. Clip closure or coverage with artificial materials for post-ESD ulcers has been attempted in order to prevent perforation [4], with favorable outcomes of a decreased incidence of delayed complications achieved [11]. However, Kato et al. reported that mucosal closure may be difficult in some tumors, such as those located in the proximal portion or those with large, occupied circumferences [12].

Mizutani et al. analyzed the risk factors for incomplete closure and found that a lesion location at the medial/anterior wall and a large lesion size were independent predictors of incomplete closure [13]. Shielding the wound with a Polyglycolic acid (PGA) sheet is also reportedly effective in avoiding delayed adverse events when complete closure of the mucosal defect is not possible. However, Fukuhara reported that the short-term outcomes of these patients were worse than those of patients who achieved complete closure [14].

Laparoscopic endoscopy cooperative surgery (LECS) is an alternative treatment approach that can achieve an appropriate resection margin and prevent duodenal leakage by reinforcing the ESD site. Kanaji et al. reported the safety and feasibility of duodenal LECS in a single-center prospective study [15], and a retrospective multicenter study confirmed its safety and feasibility [16]. However, closure of the thin wall after duodenal ESD remains challenging and requires highly advanced laparoscopic suturing techniques. Furthermore, a large mucosal defect encompassing the posterior wall or the pancreatic side can be difficult to suture, and great care should be taken to avoid causing stenosis or involving the papilla.

Although the precise mechanism underlying delayed perforation is unclear, irritating substances, such as bile and pancreatic juice, may play a crucial role [14, 17, 18]. In addition, Hanaoka et al. reported that delayed perforation after ESD occurred because of ischemic changes at the ESD site [19]. In a large animal model, we revealed that ischemia is one of the main causes of delayed perforation after duodenal ESD [20].

We previously reported the efficacy of myoblast sheet transplantation in preventing gastric perforation and pancreatic fistula in a rat model [21, 22]. In addition, we demonstrated that transplantation of autologous myoblast sheets onto the serosal site after duodenal ESD prevented delayed perforation in a porcine model [23]. This result encouraged us to conduct a clinical trial using autologous myoblast sheets in patients with SNADETs.

We therefore established this first-in-human (FIH) clinical trial, the objective of which was to evaluate the safety, efficacy, and procedural operability of this novel regenerative medicine approach involving transplanting an autologous myoblast sheet laparoscopically onto the serosa after ESD in cases with a high risk of delayed perforation.

Methods

Study design

This study was a phase 1 FIH clinical trial to assess the safety and efficacy of laparoscopic myoblast sheet transplantation after duodenal ESD. This study was conducted at a single center (Nagasaki University Hospital). No sample size calculation was used in this study, but the study included six adult patients with SNADETs. This study was supported by the Japan Agency for Medical Research and Development (AMED): the title of the approved study “An exploratory clinical trial of TERGS0001 in laparoscopic and endoscopic cooperative surgery for superficial non-ampullary duodenal epithelial tumor “, registered with the Japan Registry of Clinical Trials as jRCT2073210094, and approved by the Institutional Review Board of Nagasaki University on January 20, 2021.

This study was conducted according to the principles of the Declaration of Helsinki and the Japan Good Clinical Practice guidelines, and in compliance with the ethical guidelines for medical studies in human subjects. Written informed consent was obtained from all patients.

The inclusion and exclusion criteria are listed in Table 1. Patients ≥ 20 years old with SNADETs eligible for ESD were included in this study.

Table 1 Inclusion and exclusion criteria of the study
Full size table

Outcome measurements

These assessments are presented in Table 2. Abdominal computed tomography (CT) was performed at baseline and three days after transplantation. At baseline, the tumor size was measured using an endoscopic scale. On days 1 and 7 after transplantation, an endoscopic examination was performed to assess the size of the ulcer.

Table 2 Treatment schedule and outcome measures
Full size table

Primary endpoints

  • Efficacy outcome measures.

  1. 1)

    The onset of peritonitis due to delayed perforation within three days after surgery.

Delayed perforation was defined as a perforation that manifested clinically, such as with a fever and abdominal pain. Fluid collection and abdominal air outside the duodenum on abdominal CT suggested perforation. We clarified the definition in detail to exclude the effects of laparoscopic procedures (Fig. 1).

Fig. 1
figure 1

Flowchart for defining the occurrence of peritonitis after laparoscopic cell sheet transplantation. “Severe abdominal pain” means 1) pain that could not be relieved with the application of a strong painkiller, such as pentazocine, or 2) pain accompanied by rebound tenderness and muscle defense, as judged by two surgeons

Full size image

  • Safety outcome measures.

  1. 1)

    All adverse events during the follow-up period.

  2. 2)

    Any defect in the clinical trial product.

  3. 3)

    Any adverse event caused by a defect in the clinical trial product.

Secondary endpoints

  • Efficacy outcome measures.

  1. 1)

    Intraabdominal abscess during the follow-up period.

  2. 2)

    Postoperative drainage fluid examinations (amylase, bilirubin).

  3. 3)

    Development of epithelization or stricture on postoperative days 7 and 29.

  4. 4)

    Intraoperative procedural accidents.

  1. a)

    Perforation requiring intraoperative surgical closure.

  2. b)

    Intraoperative development of micro perforation.

  3. c)

    The onset of bleeding.

  4. d)

    Other procedural accidents.

  1. 5)

    Time spent on laparoscopic implantation of a myoblast sheet.

  2. 6)

    Success in the placement of a myoblast sheet.

  3. 7)

    Body temperature on the day after ESD and postoperative days 3 and 7.

  4. 8)

    Peripheral white blood cell counts on the day after ESD and on postoperative days 3 and 7.

  5. 9)

    CRP level on the day after ESD and postoperative days 3 and 7.

  6. 10)

    Presence or absence of abdominal pain during the follow-up period.

  7. 11)

    Presence or absence of the need for secondary emergency surgery owing to the onset of peritonitis after implantation of a skeletal muscle-derived cell sheet.

  8. 12)

    Presence or absence of bleeding requiring emergency care.

  9. 13)

    Curative resection rate in all tumors subjected to ESD.

  10. 14)

    Endoscopic mucosal resection size.

  11. 15)

    Evaluation of resected tumor specimen.

  1. a)

    Mucosal resection size.

  2. b)

    Histopathology data.

  1. 16)

    Relationship between success or failure of mucosal closure and the presence or absence of peritonitis after delayed perforation.

  • Safety outcome measures.

  1. 1)

    Serious adverse events.

  2. 2)

    Adverse events caused by harvesting skeletal muscle tissues (adverse events with an undeniable causal relationship with harvesting skeletal muscle tissues).

  3. 3)

    Changes in vital signs, complete blood count, and serum chemistry.

Autologous myoblast cell cultures and preparation of myoblast sheets

Seven weeks before duodenal ESD, approximately 2–5 g of skeletal muscle was obtained from the quadriceps muscle of each patient and transferred by air to the cell-processing faculty (CPF) of the Terumo Corporation (Kanagawa, Japan). In the CPF, the connective tissue will be carefully removed from the retrieved specimen, and the remaining muscle tissue was minced into small pieces. The muscles were digested at 37 °C in a aluminum block bath with TrypLE Select (Thermo Fisher, MA, USA) containing collagenase, gentamicin sulfate, and amphotericin B. The fluid was discarded, and culture medium (MCDB131; Thermo Fisher) supplemented with 20% fetal bovine serum was added to halt the enzymatic digestion process. Isolated cells were collected by centrifugation and then seeded onto flasks (Thermo Fisher) with MCDB131 medium (Thermo Fisher) supplemented with 20% fetal bovine serum.

After cultivation, they were harvested by trypsinization. After sufficient expansion for cell sheet fabrication, the cells were dissociated from the flasks with TrypLE Select, and the cell suspension will be cryopreserved and transferred to Nagasaki University Hospital two days before transplantation. In the CPF of Nagasaki University, the cells were reincubated on 60-mm temperature-responsive culture dishes (CellSeed, Tokyo, Japan) at 37 °C with the cell numbers adjusted to 2.2 × 107 per dish. On the day of transplantation, the cells were washed with ice-cold HBSS(+) and incubated at room temperature for 10 min. After reducing the culture temperature, the myoblast sheet could be detached without any need for enzymatic treatment, thereby preserving the important membrane proteins and extracellular matrix and allowing the cell sheet to successfully integrate with the tissue at the implanted site. The diameter of each detached cell sheet was expected to be approximately 2.5 cm. To increase strength during handling, fibrin was sprayed onto the surface of the cell sheet.

The evaluation of the myoblast sheet

The harvested myoblasts were assessed for the viable cell number and viability at every time point of passaging. Quality testing of the myoblast sheet also included assessments for the presence of bacteria, viruses, mycoplasma, or endotoxin contamination. Cell purity was measured by flow cytometry (Beckman Coulter, Miami, FL, USA) after staining with an anti-cluster of differentiation 56 antibody (CD56; BD Bioscience, San Diego, CA, USA).

Results

The initial plan was to enroll six cases in our clinical study, but due to the coronavirus pandemic, which decreased the chance of detecting SNADETs through a medical examination with gastroduodenoscopy, this trial was limited to a total of three cases.

Table 3 summarizes the perioperative characteristics of the three enrolled patients. Laparoscopic transplantation of the two cell sheets was performed without any adverse events. Although the postoperative course was uneventful in all enrolled patients in this study, our strict criteria for the postoperative amylase level in drainage fluid determined that the development of peritonitis was positive in two of three cases.

Table 3 Clinical and operative characteristics and outcomes
Full size table

Case 1

Mid 50’s woman was referred to our hospital because SNADET was detected during a routine medical checkup. Upper gastroduodenal endoscopy revealed 0-IIa type tumors in the descending duodenum. As the tumor was 25 mm in diameter, endoscopic resection was indicated because of the potential for malignancy (Fig. 2A).

Fig. 2
figure 2

Preparation of the myoblast sheet and laparoscopic transplantation of the sheet after ESD in case 1. A: Superficial duodenal tumor located in the second portion of the duodenum. B: Muscle tissue was surgically excised from the femur of the patient. C: A transplantable myoblast sheet was harvested after seven weeks of culture. D: A mucosal defect around 40 mm in diameter after duodenal ESD. E: Cell sheets were transplanted onto the serosal side of the duodenal wall after ESD. C: Abdominal CT three days after transplantation revealed no obvious signs of perforation

Full size image

Written informed consent for inclusion in our clinical study was obtained, and muscle specimens were harvested from the vastus medialis muscle under local anesthesia (Fig. 2B). The resected specimen (3.2 g) was immediately transported by air under sterile conditions to the CPF of Terumo Corporation located at Kanagawa, where all culture and cell fabrication steps mentioned above were performed.

After seven weeks of cell expansion, the cell suspensions were transported back to the CPF of Nagasaki University Hospital on the day before transplantation and placed in temperature-responsive cell culture dishes (UPCell; CellSeed) to fabricate a myoblast sheet (Fig. 2C).

Under general anesthesia, duodenal LECS was performed: five trocars were initially inserted into the abdomen for laparoscopic transplantation of cell sheets. Intraoperative endoscopy revealed 0-IIa type tumors on the oral side of the papilla of Vater, in the descending duodenum. After clamping the jejunum, duodenal ESD was performed by an endoscopist (Fig. 2D). Although immediate perforation was not evident, the wall after ESD was so thin that the endoscopic light could be seen laparoscopically.

After closure of the mucosal defect with endoscopic clipping, two myoblast sheets were transplanted onto the severe side of the duodenal ESD site using a silicon-made membranous device. A myoblast sheet on the carrier was placed onto a polyester mesh, and the carrier and mesh were pinched with conventional laparoscopic forceps and rolled up for placement into the abdominal cavity through a 12-mm laparoscopic port. The carrier and nylon mesh were deployed, and the carrier attached to the cell sheet was placed on the surface of the duodenum, cell-sheet side down. After confirming the attachment of the cell sheet, the carrier and nylon mesh were gently removed and retrieved from the intra-abdominal cavity (Fig. 2E).

On postoperative day 1, the patient did not show any signs of peritonitis, such as abdominal pain or a fever. An endoscopic examination revealed no perforation, but dropout of several clips was observed. The amylase value in the fluid of the drain placed in Morrison’s pouch was 68 U/L on postoperative day 1.

Abdominal CT on postoperative day 3 showed neither air bubbles nor fluid around the duodenum (Fig. 2F), and there were no signs of peritonitis or retroperitonitis on a physical examination. The postoperative course was uneventful, and the patient was discharged from the hospital nine days after the operation. Follow-up examinations were performed approximately seven weeks after cell sheet transplantation, including endoscopic and clinical examinations. No tests revealed any abnormalities, confirming the safety of the transplantation procedure.

Case 2

Mid 70’s man was referred to our hospital because a SNADET 25 mm in diameter was found in the second portion of the duodenum. Seven weeks after harvesting the skeletal muscle tissue, duodenal LECS was performed. Although no intraoperative perforation was observed, mucosal closure with endoscopic clips was insufficient because of the large mucosal defect after ESD (Fig. 3A). Incomplete closure of the mucosal defect by clipping causes the ulcer base to bulge outward, leaving a diverticulum-like space at the ESD site.

Fig. 3
figure 3

Myoblast sheet transplantation after ESD in case 2. A: The closure of the mucosal defect with clipping was incomplete due to its large diameter. A protruding, thinned ulcerative base after incomplete clipping was seen. B: Two cell sheets were transplanted to fully cover the thinned duodenal wall after duodenal mobilization. C: Abdominal CT three days after transplantation showed a small air bubble at the dorsal side of the duodenum. Neither massive free air nor fluid collection was evident. D: The air bubble on abdominal CT had diminished by seven days after transplantation

Full size image

As the thinned area after ESD was semicircular from the dorsal side to the contralateral side of the pancreas, full mobilization of the duodenum was performed to the extent of the pancreatic head. Two cell sheets were applied to the protruding area of the ESD, and the omentum was placed onto the transplanted sheets (Fig. 3B).

Although there was no postoperative abdominal pain or a fever, an elevated intra-abdominal drain amylase level of 15,623 U/ml was observed the following day, and abdominal CT revealed a small amount of free air along the dorsal duodenum of the ESD site.

With close follow-up, the patient’s condition did not deteriorate, and the volume of drainage fluid and amylase value promptly decreased to 551 U/ml on the third day (Fig. 4A). Abdominal CT still showed free air bubbles on the dorsal side of the duodenum (Fig. 3C). There was no worsening of clinical symptoms, such as abdominal pain, and free air had completely diminished on abdominal CT (Fig. 3D).

Fig. 4
figure 4

Postoperative changes in the excretion volume obtained from the drainage tube placed near the transplanted site in cases 2 (A) and 3 (B)

Full size image

On the 49th day after transplantation, endoscopic observation revealed no stenosis in the transplanted area.

Case 3

Early 40’s man with a SNADET located in the descending portion of the duodenum was included in our study. During duodenal LECS, at least four micro perforations were observed intraoperatively; however, each perforation was small and not as severe as that accompanied by mucosal exfoliation (Fig. 5A). Air and bile leakage diminished after complete mucosal closure with endoscopic clips, where a thin ulcer base bulged outward like a diverticulum due to mucosal clips (Fig. 5B). Two cell sheets were applied to the serosa side after ESD to cover each perforated site, and the omentum was placed onto the sheets without fixation (Fig. 5C). No postoperative abdominal pain or a fever was observed; however, a blood test revealed a CRP level of 13 mg/dl.

Fig. 5
figure 5

Myoblast sheet transplantation after ESD in case 3. A: At least four micro perforations were evident after duodenal ESD. B: Laparoscopy also revealed micro perforations with bile leakage. The thinned duodenal wall was protruding like the diverticulum due to mucosal clipping. C: All micro perforations were completely sealed by transplanted myoblast sheets. D: Abdominal CT three days after transplantation showed a small air bubble with diverticulum-like protrusion of the duodenal wall. Fluid collection around the duodenum was not evident. E: Endoscopy seven days after transplantation revealed an opening of the mucosa with dropout of intraoperative clipping. F: Abdominal CT seven days after transplantation showed air bubbles along the duodenal wall, but they had not extended even after endoscopic observation

Full size image

The patient did not show any symptoms of peritonitis; however, an elevated drain amylase level of 16,109 U/ml was observed on postoperative day 3 (Fig. 4B). CT of the abdomen showed no marked increase in fluid collection and free air, but a small air bubble was detected on the lateral side of the ESD site (Fig. 5D). Two possibilities were considered as the source of this solitary air bubble: localized free air due to perforation or a diverticulum-like space in the duodenum. Since there were no worsening clinical symptoms, such as a fever or increased abdominal pain, protease inhibitors, somatostatin analog, and antibiotics were started for pancreatitis, resulting in a prompt reduction of the drain amylase level to 1,973 U/ml (Fig. 4B). On day 7, an endoscopic examination revealed an opening of the mucosa, which was closed intraoperatively with clips (Fig. 5E). A robust structure backing the bottom of the defect of the duodenal wall was observed, and abdominal CT after endoscopy showed no expansion of free air despite endoscopic insufflation (Fig. 5F).

On the 49th (50th) day after transplantation, endoscopic observation revealed no stenosis in the transplanted cell sheet area.

Discussion

This clinical trial evaluated the efficacy, safety, and feasibility of laparoscopic transplantation of autologous myoblast sheets to prevent delayed perforation after duodenal ESD in patients with SNADETs. In this study, we showed that cell sheet transplantation was able to partially prevent postoperative perforation after duodenal ESD, even in patients with an increased risk of delayed perforation, such as those with incomplete mucosal closure and intraoperative micro perforations due to a larger tumor size than expected. The novelty lies in the fact that cell sheet transplantation was performed on the serosal side using a laparoscope and not on the inner lumen using an endoscope. The results obtained in this study are remarkable, as they can expand the applications of cell sheet medicine from the endoscopic field to the laparoscopic surgical field.

Recent advances in tissue engineering have enabled the use of cells as a promising modality for treating patients with intractable diseases. Cell therapy has recently been introduced into clinical practice for the functional repair of deficiencies in various fields, including hepatology and gastroenterology [24,25,26,27,28,29,30,31]. Cell sheet medicine has also been implemented in clinical practice in the field of cardiac surgery. Yoshikawa et al. reported that the advantages of cell sheet implantation include the potential to increase the number of implanted cells and the maintenance of cell-to-cell contact, which leads to a greater survival of cells, as well as a better paracrine effect than local injection [32, 33], which is thought to be the main mechanism involved in cell sheet transplantation. The secretion of various growth factors, such as hepatocyte growth factor (HGF) and vascular endothelial growth factor (VEGF), can locally promote angiogenesis at the transplanted site. We also recently indicated that myoblast sheet transplantation accelerates healing of the transplanted site through the chronological expression of various growth factors [34]. Based on the above findings, one of the mechanisms of cell sheet transplantation may therefore directly promote the regeneration of a thinned duodenal wall, and thereby strengthen the adhesion around the ESD site. In addition to these paracrine effects, the barrier effect of the myoblast sheet might also play a key role in the prevention of delayed perforation.

Cell sheet technology has also been applied in clinical practice in gastrointestinal fields as well. Ohki et al. conducted a clinical study of 10 patients in whom autologous buccal cell sheets were transplanted to cover mucosal defects after ESD for superficial esophageal cancer [35]. They demonstrated remarkable results in which cell sheet transplantation successfully prevented esophageal stricture, even after semi-circumferential ESD with a high risk of stenosis. In collaboration with Ohki et al., Yamaguchi et al. investigated the efficacy of cell sheet transplantation after airplane transportation of a fabricated cell sheet. Despite their study involving a wider resection area and lower cell sheet coverage for the ESD than those reported by Ohki et al., they also showed impressive results with a luminal stenosis rate of 40% and a median number of mandatory endoscopic dilatations of 0 [36]. Recently, the effect of cell sheet transplantation on post-anastomotic stenosis in congenital esophageal disease has also been reported. Among the three patients who received autologous oral mucosal cell sheets after endoscopic balloon dilatation for anastomotic stenosis, two were free from dilatation for at least one year after transplantation [37]. Regarding transplantation onto the esophageal lumen as described above, transplantation of the myoblast sheet directly into the mucosa under endoscopy seems to be an attractive option, however, there is still no available feasible device that allows us to transport a fragile cell sheet into the duodenum over the narrow esophagus, the esophagogastric junction and the pylorus. Moreover, the presence of bile, pancreatic juice and active peristalsis of the duodenal lumen is well known to make the engraftment of such a fragile cell sheet onto the ESD ulcer very difficult.

Various routes of cell transplantation, such as intravascular and local injection, are considered, depending on the site where the implanted cells are expected to function. Our study is important in that we successfully transported fragile myoblast sheets into a high-pressure pneumoperitonized abdominal cavity through a thin laparoscopic trocar. In endoscopic transplantation of cell sheets into the inner lumen of the esophagus, surgical intervention to create a specified route for transplantation is not required, and endoscopically applying a sheet-like structure is likely to be relatively easy with a simple procedure [37,38,39]. However, transplantation into the inner body, such as the thoracic and abdominal cavities, requires a surgical route for cell sheet transplantation.

In a clinical study of autologous myoblast sheet transplantation in 15 ischemic cardiomyopathy patients, the cell sheets were transplanted onto the left ventricular wall of the heart through thoracotomy of the left fifth intercostal space [40]. Kanzaki et al. reported thoracoscopic transplantation of an autologous dermal fibroblast sheet after lung resection to treat air leakage [41, 42]. They used a CellShifter with thoracoscopic equipment for transplantation through a trocar inserted into the thoracic wall. Recently, various thoracoscopic devices have been developed for the transplantation of sheets into bone-supported thoracic cavities. However, no reports have described the transplantation of sheets into a high-pressure pneumoperitonized abdominal cavity [43, 44]. We established a feasible procedure for laparoscopic transplantation of myoblast sheets in our porcine model [45] and confirmed that this procedure is also practical for clinical settings. This approach may expand the potential utility of cell sheet medicine to intra-abdominal organs other than the duodenum.

Despite inadequate endoscopic clip closure of a large mucosal defect (> 40 mm) in case 2 and multiple intraoperative micro perforations in case 3, cell sheet transplantation in both cases was able to prevent clinical problems, such as increased drainage or worsening of peritonitis symptoms due to rupture of the ulcer base. In case 2, we were unable to exclude the possibility that the elevation of drainage amylase might have been due to pancreatitis caused by Kocher’s maneuver extending to the dorsal side of the pancreas, rather than due to delayed perforation. It might also be possible that the micro perforations in case 3 were not completely covered by the cell sheet, as we stipulated that we could use only two cell sheets. As the patient’s postoperative condition did not deteriorate, mandatory reoperation for cure was not necessary. Although we should judge “peritonitis” occurred in two of three patients along with our criteria, our findings indicate the effectiveness of cell sheet transplantation for reinforcement of defects of the intestinal wall, even with exposure to irritant digestive juice.

An endoscopic examination revealed a robust structure backing the mucosal defect seven days after ESD in case 3. This finding was similar to those obtained in our animal model, indicating massive fibroblasts and collagen fibers among the implanted myoblast backing diminished ulcers based on an immunohistochemical examination at the ESD site [23]. In all cases, neither stenosis nor any recurrent tumor was found on endoscopy at 49 days after transplantation, which indicates that the paracrine effect of the cell sheet did not negatively impact the process of tissue regeneration.

With its recent national health insurance coverage, duodenal LECS accompanied by suture reinforcement of thin ESD sites has rapidly become popular. Proper candidates for cell sheet transplantation in the future may be patients in whom laparoscopic suture after ESD would be difficult due to the tumor being located on the pancreatic side and in cases of large lesions where there is concern about duodenal stricture. A randomized control trial is needed to validate the effectiveness of cell sheet transplantation for patients who truly benefit from this treatment.

However, we are concerned that there would be hurdles in the accumulation and registration of suitable patients for such a study. First, the subjects of that future study might be those with SNADETs without extension to the submucosa; however, the preoperative diagnosis of the depth of invasion with SNADETs as well as their histological diagnosis is tremendously difficult. In the present study, because tumors were judged as invading into deeper layers over the mucosa with preoperative endoscopy, three patients were excluded from enrollment before the eligibility committee (data not shown). However, a postoperative pathological examination after duodenal LECS revealed that one was an adenoma, and the other two were duodenal carcinomas confined to the mucosa. Second, a large discrepancy was found between the preoperative endoscopic tumor diameter measurement and the actual resected tumor diameter upon ESD, which might have led to the unpredictability of the difficulty of ESD and mucosal clipping after ESD. This uncertainty in the ESD procedure might interfere with the equal distribution of patients in randomized control trials.

There have been several reports on the application of cell sheet technology in the abdominal cavity. Maruya et al. reported that adipose-derived stem cell (ADSC) sheets enhanced anastomotic strength in a miniature pig model [46]. Hara et al. similarly reported the efficacy of ADSC sheet transplantation around the anastomotic site of the bile duct to prevent biliary anastomotic stricture [47]. In addition, we previously reported that cell sheets fabricated with islets, fibroblasts, or ADSCs had a cytoprotective effect on the islet function compared to sheets fabricated with islets alone [48]. These sheets may be transplanted onto the liver surface, as described by Inagaki et al. [49]. Miyamoto also reported that hepatocyte sheets transplanted onto the liver surface exerted sustained liver function stimulation compared with sheets transplanted subcutaneously [50].

Several limitations associated with the present study warrant mention. Because of the impact of the spread of the new coronavirus infection, this trial was limited to a total of only three cases, and because the procedure was performed for prevention, statistical consideration of efficacy is difficult. It was also not possible to rule out the influence of the small sample size on the study outcome and power calculations could therefore not performed to support the usefulness of this study..In addition, our clinical study was designed as a single-arm, single-center study that may have affected the outcome of therapy. A randomized control trial seemed difficult to perform, as described above, so future studies with more patients are needed.

Conclusions

Our ongoing FIH clinical trial demonstrated successful transplantation of autologous myoblast sheets into pneumoperitonized abdominal cavities via the laparoscopic approach. These results suggest the potential application of cell sheet medicine in the treatment of various abdominal organs with minimal invasiveness. The efficacy of cell sheet transplantation for preventing delayed perforation after duodenal ESD must be further validated in subsequent clinical trials.

Data availability

The data that support the findings of this study are available from the corresponding author on reasonable request.

References

  1. Kakushima N, Kanemoto H, Tanaka M, Takizawa K, Ono H. Treatment for superficial non-ampullary duodenal epithelial tumors. World J Gastroenterol. 2014;20:12501–8.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Goda K, Kikuchi D, Yamamoto Y, Takimoto K, Kakushima N, Morita Y, et al. Endoscopic diagnosis of superficial non-ampullary duodenal epithelial tumors in Japan: Multicenter case series. Dig Endosc. 2014;26(Suppl 2):23–9.

    Article  PubMed  Google Scholar 

  3. Yoshida M, Yabuuchi Y, Kakushima N, Kato M, Iguchi M, Yamamoto Y, et al. The incidence of non-ampullary duodenal cancer in Japan: the first analysis of a national cancer registry. J Gastroenterol Hepatol. 2021;36:1216–21.

    Article  PubMed  Google Scholar 

  4. Nakagawa K, Sho M, Fujishiro M, Kakushima N, Horimatsu T, Okada KI et al. Clinical practice guidelines for duodenal cancer 2021. J Gastroenterol. 2022.

  5. Yabuuchi Y, Yoshida M, Kakushima N, Kato M, Iguchi M, Yamamoto Y, et al. Risk factors for non-ampullary duodenal adenocarcinoma: a systematic review. Dig Dis. 2022;40:147–55.

    Article  PubMed  Google Scholar 

  6. Okada K, Fujisaki J, Kasuga A, Omae M, Kubota M, Hirasawa T, et al. Sporadic nonampullary duodenal adenoma in the natural history of duodenal cancer: a study of follow-up surveillance. Am J Gastroenterol. 2011;106:357–64.

    Article  PubMed  Google Scholar 

  7. Fujihara S, Mori H, Kobara H, Nishiyama N, Matsunaga T, Ayaki M, et al. Management of a large mucosal defect after duodenal endoscopic resection. World J Gastroenterol. 2016;22:6595–609.

    Article  PubMed  PubMed Central  Google Scholar 

  8. Inoue T, Uedo N, Yamashina T, Yamamoto S, Hanaoka N, Takeuchi Y, et al. Delayed perforation: a hazardous complication of endoscopic resection for non-ampullary duodenal neoplasm. Dig Endosc. 2014;26:220–7.

    Article  PubMed  Google Scholar 

  9. Nonaka S, Oda I, Tada K, Mori G, Sato Y, Abe S, et al. Clinical outcome of endoscopic resection for nonampullary duodenal tumors. Endoscopy. 2015;47:129–35.

    PubMed  Google Scholar 

  10. Hara Y, Goda K, Dobashi A, Ohya TR, Kato M, Sumiyama K, et al. Short- and long-term outcomes of endoscopically treated superficial non-ampullary duodenal epithelial tumors. World J Gastroenterol. 2019;25:707–18.

    Article  PubMed  PubMed Central  Google Scholar 

  11. Tsutsumi K, Kato M, Kakushima N, Iguchi M, Yamamoto Y, Kanetaka K, et al. Efficacy of endoscopic preventive procedures to reduce delayed adverse events after endoscopic resection of superficial nonampullary duodenal epithelial tumors: a meta-analysis of observational comparative trials. Gastrointest Endosc. 2021;93:367–74. e3.

    Article  PubMed  Google Scholar 

  12. Kato M, Ochiai Y, Fukuhara S, Maehata T, Sasaki M, Kiguchi Y, et al. Clinical impact of closure of the mucosal defect after duodenal endoscopic submucosal dissection. Gastrointest Endosc. 2019;89:87–93.

    Article  PubMed  Google Scholar 

  13. Mizutani M, Kato M, Sasaki M, Masunaga T, Kubosawa Y, Hayashi Y, et al. Predictors of technical difficulty for complete closure of mucosal defects after duodenal endoscopic resection. Gastrointest Endosc. 2021;94:786–94.

    Article  PubMed  Google Scholar 

  14. Fukuhara S, Kato M, Iwasaki E, Sasaki M, Tsutsumi K, Kiguchi Y, et al. Management of perforation related to endoscopic submucosal dissection for superficial duodenal epithelial tumors. Gastrointest Endosc. 2020;91:1129–37.

    Article  PubMed  Google Scholar 

  15. Kanaji S, Morita Y, Yamazaki Y, Otowa Y, Takao T, Tanaka S, et al. Feasibility of laparoscopic endoscopic cooperative surgery for non-ampullary superficial duodenal neoplasms: single-arm confirmatory trial. Dig Endosc. 2021;33:373–80.

    Article  PubMed  Google Scholar 

  16. Nunobe S, Ri M, Yamazaki K, Uraoka M, Ohata K, Kitazono I, et al. Safety and feasibility of laparoscopic and endoscopic cooperative surgery for duodenal neoplasm: a retrospective multicenter study. Endoscopy. 2021;53:1065–8.

    Article  PubMed  Google Scholar 

  17. Hoteya S, Kaise M, Iizuka T, Ogawa O, Mitani T, Matsui A, et al. Delayed bleeding after endoscopic submucosal dissection for non-ampullary superficial duodenal neoplasias might be prevented by prophylactic endoscopic closure: analysis of risk factors. Dig Endosc. 2015;27:323–30.

    Article  PubMed  Google Scholar 

  18. Yahagi N, Kato M, Ochiai Y, Maehata T, Sasaki M, Kiguchi Y, et al. Outcomes of endoscopic resection for superficial duodenal epithelial neoplasia. Gastrointest Endosc. 2018;88:676–82.

    Article  PubMed  Google Scholar 

  19. Hanaoka N, Uedo N, Ishihara R, Higashino K, Takeuchi Y, Inoue T, et al. Clinical features and outcomes of delayed perforation after endoscopic submucosal dissection for early gastric cancer. Endoscopy. 2010;42:1112–5.

    Article  CAS  PubMed  Google Scholar 

  20. Hashiguchi K, Maruya Y, Matsumoto R, Yamaguchi S, Ogihara K, Ohnita K, et al. Establishment of an in-vivo porcine delayed perforation model after duodenal endoscopic submucosal dissection. Dig Endosc. 2021;33:381–9.

    Article  PubMed  Google Scholar 

  21. Tanaka T, Kuroki T, Adachi T, Ono S, Kitasato A, Hirabaru M, et al. Development of a novel rat model with pancreatic fistula and the prevention of this complication using tissue-engineered myoblast sheets. J Gastroenterol. 2013;48:1081–9.

    Article  CAS  PubMed  Google Scholar 

  22. Tanaka S, Kanetaka K, Fujii M, Ito S, Sakai Y, Kobayashi S, et al. Cell sheet technology for the regeneration of gastrointestinal tissue using a novel gastric perforation rat model. Surg Today. 2017;47:114–21.

    Article  PubMed  Google Scholar 

  23. Matsumoto R, Kanetaka K, Maruya Y, Yamaguchi S, Kobayashi S, Miyamoto D, et al. The efficacy of autologous myoblast sheet transplantation to prevent Perforation after Duodenal Endoscopic Submucosal Dissection in Porcine Model. Cell Transpl. 2020;29:963689720963882.

    Article  Google Scholar 

  24. Terai S, Ishikawa T, Omori K, Aoyama K, Marumoto Y, Urata Y, et al. Improved liver function in patients with liver cirrhosis after autologous bone marrow cell infusion therapy. Stem Cells. 2006;24:2292–8.

    Article  CAS  PubMed  Google Scholar 

  25. Voltarelli JC, Couri CE, Stracieri AB, Oliveira MC, Moraes DA, Pieroni F, et al. Autologous nonmyeloablative hematopoietic stem cell transplantation in newly diagnosed type 1 diabetes mellitus. JAMA. 2007;297:1568–76.

    Article  CAS  PubMed  Google Scholar 

  26. Cassinotti A, Annaloro C, Ardizzone S, Onida F, Della Volpe A, Clerici M, et al. Autologous haematopoietic stem cell transplantation without CD34 + cell selection in refractory Crohn’s disease. Gut. 2008;57:211–7.

    Article  CAS  PubMed  Google Scholar 

  27. Hawkey CJ, Allez M, Clark MM, Labopin M, Lindsay JO, Ricart E, et al. Autologous hematopoetic stem cell transplantation for refractory Crohn Disease: a Randomized Clinical Trial. JAMA. 2015;314:2524–34.

    Article  CAS  PubMed  Google Scholar 

  28. Bhansali S, Dutta P, Kumar V, Yadav MK, Jain A, Mudaliar S, et al. Efficacy of autologous bone marrow-derived mesenchymal stem cell and mononuclear cell transplantation in type 2 diabetes Mellitus: a Randomized, Placebo-controlled comparative study. Stem Cells Dev. 2017;26:471–81.

    Article  CAS  PubMed  Google Scholar 

  29. Newsome PN, Fox R, King AL, Barton D, Than NN, Moore J, et al. Granulocyte colony-stimulating factor and autologous CD133-positive stem-cell therapy in liver cirrhosis (REALISTIC): an open-label, randomised, controlled phase 2 trial. Lancet Gastroenterol Hepatol. 2018;3:25–36.

    Article  PubMed  Google Scholar 

  30. Panes J, Garcia-Olmo D, Van Assche G, Colombel JF, Reinisch W, Baumgart DC, et al. Long-term efficacy and safety of Stem Cell Therapy (Cx601) for Complex Perianal Fistulas in patients with Crohn’s Disease. Gastroenterology. 2018;154:1334–42. e4.

    Article  PubMed  Google Scholar 

  31. Moroni F, Dwyer BJ, Graham C, Pass C, Bailey L, Ritchie L, et al. Safety profile of autologous macrophage therapy for liver cirrhosis. Nat Med. 2019;25:1560–5.

    Article  CAS  PubMed  Google Scholar 

  32. Menasche P, Alfieri O, Janssens S, McKenna W, Reichenspurner H, Trinquart L, et al. The myoblast autologous grafting in ischemic cardiomyopathy (MAGIC) trial: first randomized placebo-controlled study of myoblast transplantation. Circulation. 2008;117:1189–200.

    Article  PubMed  Google Scholar 

  33. Yoshikawa Y, Miyagawa S, Toda K, Saito A, Sakata Y, Sawa Y. Myocardial regenerative therapy using a scaffold-free skeletal-muscle-derived cell sheet in patients with dilated cardiomyopathy even under a left ventricular assist device: a safety and feasibility study. Surg Today. 2018;48:200–10.

    Article  CAS  PubMed  Google Scholar 

  34. Yamaguchi S, Higashi M, Kanetaka K, Maruya Y, Kobayashi S, Hashiguchi K, et al. Rapid and chronological expression of angiogenetic genes is a major mechanism involved in cell sheet transplantation in a rat gastric ulcer model. Regen Ther. 2022;21:372–9.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Ohki T, Yamato M, Ota M, Takagi R, Murakami D, Kondo M, et al. Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets. Gastroenterology. 2012;143:582–8. e2.

    Article  PubMed  Google Scholar 

  36. Yamaguchi N, Isomoto H, Kobayashi S, Kanai N, Kanetaka K, Sakai Y, et al. Oral epithelial cell sheets engraftment for esophageal strictures after endoscopic submucosal dissection of squamous cell carcinoma and airplane transportation. Sci Rep. 2017;7:17460.

    Article  PubMed  PubMed Central  Google Scholar 

  37. Fujino A, Fuchimoto Y, Baba Y, Isogawa N, Iwata T, Arai K, et al. First-in-human autologous oral mucosal epithelial sheet transplantation to prevent anastomotic re-stenosis in congenital esophageal atresia. Stem Cell Res Ther. 2022;13:35.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  38. Maeda M, Kanai N, Kobayashi S, Hosoi T, Takagi R, Ohki T, et al. Endoscopic cell sheet transplantation device developed by using a 3-dimensional printer and its feasibility evaluation in a porcine model. Gastrointest Endosc. 2015;82:147–52.

    Article  PubMed  Google Scholar 

  39. Kamao H, Mandai M, Ohashi W, Hirami Y, Kurimoto Y, Kiryu J, et al. Evaluation of the Surgical device and Procedure for Extracellular Matrix-Scaffold-supported human iPSC-Derived retinal pigment epithelium cell sheet transplantation. Invest Ophthalmol Vis Sci. 2017;58:211–20.

    Article  PubMed  Google Scholar 

  40. Miyagawa S, Domae K, Yoshikawa Y, Fukushima S, Nakamura T, Saito A et al. Phase I clinical trial of autologous stem cell-sheet transplantation therapy for treating cardiomyopathy. J Am Heart Assoc. 2017;6.

  41. Kanzaki M, Takagi R, Washio K, Kokubo M, Yamato M. Bio-artificial pleura using an autologous dermal fibroblast sheet. NPJ Regen Med. 2017;2:26.

    Article  PubMed  PubMed Central  Google Scholar 

  42. Kanzaki M, Takagi R, Washio K, Kokubo M, Mitsuboshi S, Isaka T, et al. Bio-artificial pleura using autologous dermal fibroblast sheets to mitigate air leaks during thoracoscopic lung resection. NPJ Regen Med. 2021;6:2.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  43. Maeda M, Yamato M, Kanzaki M, Iseki H, Okano T. Thoracoscopic cell sheet transplantation with a novel device. J Tissue Eng Regen Med. 2009;3:255–9.

    Article  CAS  PubMed  Google Scholar 

  44. Osada H, Ho WJ, Yamashita H, Yamazaki K, Ikeda T, Minatoya K, et al. Novel device prototyping for endoscopic cell sheet transplantation using a three-dimensional printed simulator. Regen Ther. 2020;15:258–64.

    Article  PubMed  PubMed Central  Google Scholar 

  45. Yamaguchi S, Kanetaka K, Maruya Y, Higashi M, Kobayashi S, Hashiguchi K, et al. Highly feasible procedure for laparoscopic transplantation of cell sheets under pneumoperitoneum in porcine model. Surg Endosc. 2022;36:3911–9.

    Article  PubMed  Google Scholar 

  46. Maruya Y, Kanai N, Kobayashi S, Koshino K, Okano T, Eguchi S, et al. Autologous adipose-derived stem cell sheets enhance the strength of intestinal anastomosis. Regen Ther. 2017;7:24–33.

    Article  PubMed  PubMed Central  Google Scholar 

  47. Hara T, Soyama A, Adachi T, Kobayashi S, Sakai Y, Maruya Y, et al. Ameliorated healing of biliary anastomosis by autologous adipose-derived stem cell sheets. Regen Ther. 2020;14:79–86.

    Article  PubMed  PubMed Central  Google Scholar 

  48. Yamashita M, Adachi T, Adachi T, Ono S, Matsumura N, Maekawa K, et al. Subcutaneous transplantation of engineered islet/adipose-derived mesenchymal stem cell sheets in diabetic pigs with total pancreatectomy. Regen Ther. 2021;16:42–52.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  49. Inagaki A, Imura T, Nakamura Y, Ohashi K, Goto M. The liver surface is an attractive transplant site for pancreatic islet transplantation. J Clin Med. 2021;10.

  50. Miyamoto D, Sakai Y, Huang Y, Yamasaki C, Tateno C, Hasegawa H, et al. Functional changes of cocultured hepatocyte sheets subjected to continuous liver regeneration stimulation in cDNA-uPA/SCID mouse: differences in transplantation sites. Regen Ther. 2021;18:7–11.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

We thank Ms. Tomomi Murai, Ms. Hideko Hasegawa and Dr. Daisuke Miyamoto for helpful discussions and technical support.

Funding

This research was supported by AMED under Grant Number JP20bk0104112 (Kengo Kanetaka).

Author information

Authors and Affiliations

  1. Tissue Engineering and Regenerative Therapeutics in Gastrointestinal Surgery, Nagasaki University Graduate School of Biomedical Sciences, Sakamoto 1-7-1, 8528102, Nagasaki, Japan

    Kengo Kanetaka, Yasuhiro Maruya & Miki Higashi

  2. Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

    Shun Yamaguchi, Ryo Matsumoto, Shinichiro Kobayashi & Susumu Eguchi

  3. Department of Gastroenterology and Hepatology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan

    Keiichi Hashiguchi & Kazuhiko Nakao

  4. Terumo Corporation, Shibuya, Japan

    Fumiya Oohashi, Masaki Matsumura & Takahiro Naka

  5. Department of Chemical Engineering, Faculty of Engineering, Graduate School, Kyushu University, Fukuoka, Japan

    Yusuke Sakai

  6. Department of Cardiovascular Surgery, Osaka University Graduate School of Medicine, Osaka, Japan

    Shigeru Miyagawa

Authors
  1. Kengo Kanetaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yasuhiro Maruya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Miki Higashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shun Yamaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Ryo Matsumoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Shinichiro Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Keiichi Hashiguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Fumiya Oohashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Masaki Matsumura
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Takahiro Naka
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Yusuke Sakai
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Kazuhiko Nakao
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Shigeru Miyagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Susumu Eguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Conceptualization: Kengo Kanetaka, Shigeru Miyagawa, Susumu Eguchi. Operative Procedure: Kengo Kanetaka, Yasuhiro Maruya, Shinichiro Kobayashi, Hashiguchi Keiichi. Cell Processing: Miki Higashi, Fumiya Oohashi, Masaki Matsumura, Takahiro Naka. Device development: Yusuke Sakai. Writing-original draft preparation: Ryo Matsumoto, Shun Yamaguchi. Writing-review and editing: Kengo Kanetaka.Surpervision: Susumu Eguchi, Kazuhiko Nakao.

Corresponding author

Correspondence to Kengo Kanetaka.

Ethics declarations

Ethics approval and consent to participate

The title of the approved study is “An exploratory clinical trial of TERGS0001 in laparoscopic and endoscopic cooperative surgery for superficial non-ampullary duodenal epithelial tumor “, registered with the Japan Registry of Clinical Trials as jRCT2073210094, and approved by the Institutional Review Board of Nagasaki University on January 20, 2021. Human cells in this study were utilized in full compliance with the Ethical Guidelines for Medical and Health Research involving Human Subjects (Ministry of Health, Labor, and Welfare (MHLW), Japan; Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan). Informed consent for publication were obtained from all patients in Japanese text.

Competing interests

Drs. Matsumoto, Yamaguchi, Kobayashi, Hashiguchi, Nakao, and Eguchi have no conflicts of interest or financial ties to declare. Drs. Higashi, Kanetaka, and Maruya received funding for cooperative research on cell sheets from the Terumo Corporation.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Kanetaka, K., Maruya, Y., Higashi, M. et al. A first-in-human clinical study of laparoscopic autologous myoblast sheet transplantation to prevent delayed perforation after duodenal endoscopic mucosal dissection. Stem Cell Res Ther 15, 117 (2024). https://doi.org/10.1186/s13287-024-03730-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1186/s13287-024-03730-3

Keywords

Stem Cell Research & Therapy

ISSN: 1757-6512