Figure 2
From: Toward a 3D model of human brain development for studying gene/environment interactions
Three-dimensional aggregates formed during different protocols for neuro-differentiation. Phase contrast images show (a) rosettes, (b) single human neural progenitor cells (hNPCs) in adherent cultures, (c) aggregates obtained from single hNPCs (Single Agg), and (d) three-dimensional aggregates derived from rosettes (Rosettes Agg). The lower panel shows the relative expression of different genes (neuronal markers: β-tubulin III (Tubb3), neurofilament heavy chain (Nefh), tyrosine hydroxylase (Th), synapsin 1 (Syn1), solute carrier family 18 (vesicular monoamine; Slc18a2); neural precursor marker: paired box gene 6 (Pax6)) in hNPCs and in aggregates derived from rosettes (Rosette Agg) and in aggregates obtained from single hNPCs (Single Agg) after 4 weeks in differentiating media. *Statistically different from the NPCs, P <0.001 Student's t test).