Table 2 Doubling times, specific metabolic rates and yield ratios of hESCs in MC-Sp in propagation and differentiation phases

Double time (hours)

27 ± 2

128 ± 14

28 ± 2

151 ± 1

Specific consumption and production rate (mmol/10⁹ cells/hour)

 qGlc

0.36 ± 0.04

0.24 ± 0.02

0.34 ± 0.05

0.18 ± 0.02

 qLac

0.63 ± 0.12

0.32 ± 0.02

0.57 ± 0.08

0.23 ± 0.01

 qGln

0.036 ± 0.003

0.033 ± 0.001

0.028 ± 0.006

0.029 ± 0.003

 qAmm

0.029 ± 0.003

0.023 ± 0.002

0.019 ± 0.005

0.016 ± 0.002

Yield ratio

 Y_Lac/Glc

1.72 ± 0.14*

1.34 ± 0.04*

1.66 ± 0.01*

1.27 ± 0.06*

 Y_Amm/Gln

0.80 ± 0.02

0.70 ± 0.04

0.67 ± 0.03

0.55 ± 0.01