Human amnion epithelial cells (hAECs) induce transcription of FoxP3
in non-regulatory T cells (non-Tregs) and promote Treg expansion
. (A) Representative images of green fluorescent protein-positive (GFP+) cells within the lung parenchyma of Rag1
-/- mice following adoptive transfer of Tregs (scale bar = 50 μm) (DAPI, blue; FoxP3, green; auto-fluorescence, red). Green staining in the absence of co-localisation with red indicates that the cells are GFP+ Tregs rather than autofluorescent cells. (B) Co-administration of hAECs and adoptive transfer of Tregs resulted in expansion of Tregs in vivo as well as some conversion of non-Tregs into GFP-expressing FoxP3+ Tregs (**P <0.01). DAPI, 4′,6-diamidino-2-phenylindole.