- Open Access
CRISPR-targeted genome editing of mesenchymal stem cell-derived therapies for type 1 diabetes: a path to clinical success?
Stem Cell Research & Therapy volume 8, Article number: 62 (2017)
Due to their ease of isolation, differentiation capabilities, and immunomodulatory properties, the therapeutic potential of mesenchymal stem cells (MSCs) has been assessed in numerous pre-clinical and clinical settings. Currently, whole pancreas or islet transplantation is the only cure for people with type 1 diabetes (T1D) and, due to the autoimmune nature of the disease, MSCs have been utilised either natively or transdifferentiated into insulin-producing cells (IPCs) as an alternative treatment. However, the initial success in pre-clinical animal models has not translated into successful clinical outcomes. Thus, this review will summarise the current state of MSC-derived therapies for the treatment of T1D in both the pre-clinical and clinical setting, in particular their use as an immunomodulatory therapy and targets for the generation of IPCs via gene modification. In this review, we highlight the limitations of current clinical trials of MSCs for the treatment of T1D, and suggest the novel clustered regularly interspaced short palindromic repeat (CRISPR) gene-editing technology and improved clinical trial design as strategies to translate pre-clinical success to the clinical setting.
Type 1 diabetes (T1D) results from an organ-specific autoimmune-mediated loss of insulin-secreting β cells in the pancreas. People with T1D manage their blood glucose levels using exogenous insulin therapy; however, this does not eliminate the development of long-term diabetic complications such as retinopathy, nephropathy, and neuropathy . In addition, life-long use of exogenous insulin increases the risk of hypoglycaemic unawareness which can be potentially life-threatening.
Currently, pancreas or islet transplantation remains the only cure; however, these treatments are limited by a shortage of donor organs and the requirement for life-long immunosuppression . An alternative to current therapies has been the generation of surrogate β cells from a variety of tissue sources via chemically or genetically induced transdifferentiation [3–10]. However, many surrogate β-cell solutions that are derived from cells such as induced pluripotent  and embryonic stem cells  are susceptible to allorejection or recurrent autoimmunity. Thus, long-term survival of transplanted cells requires encapsulation which does not necessarily provide immune protection in all cases. Due to their high plasticity, immunomodulatory properties, and fewer ethical concerns, mesenchymal stem cells (MSCs) are an attractive alternative target cell for the autologous and allogeneic treatment of T1D without the requirement for encapsulation. In addition, early studies demonstrating the ability of MSCs to differentiate into insulin-producing cells (IPCs) via ex vivo chemical induction [6–10] or various gene therapy approaches [12, 13] qualifies them as ideal candidates for cell transplantation.
The purpose of this review is to highlight the success of MSC-derived therapies in pre-clinical models of diabetes and reflect on the failure of the translation of these studies into the clinical setting. We therefore suggest the utilisation of clustered regularly interspaced short palindromic repeat (CRISPR) and implementing improved clinical trial design for the future success of T1D MSC-derived therapies.
MSCs as an immunomodulatory therapy for T1D
Within the context of T1D, MSCs have been assessed for use as an immunomodulatory therapy in small animal models of diabetes such as non-obese diabetic (NOD) mice [14–16] and streptozotocin (STZ)-induced diabetic animals [17, 18], where improvements in the glycaemic control of treated animals have been observed. Through these early studies, MSC interventions demonstrated improved T1D outcomes through two mechanisms: (i) MSC migration to areas of pancreatic injury and modification of the islet microenvironment to promote the survival and regeneration of surviving β cells; and (ii) abrogating inherent autoimmunity against β cells . In addition, MSCs can be co-transplanted with pancreatic islets, thereby protecting the islets from allogeneic immune responses . The success of MSC infusions in pre-clinical animal models of diabetes are summarised in Table 1.
MSCs promote the survival and regeneration of existing β cells
Since MSCs are capable of modifying the tissue microenvironment, MSC infusions can promote the survival and regeneration of existing β cells, leading to increases in β-cell mass and restoration of normoglycaemia [21–24]. In fact, following intravenous injection of MSCs into diabetic mice, increases in insulin levels and reduced hyperglycaemia were observed. Similarly, a single treatment of umbilical cord-MSCs in humans provided lasting reversal of autoimmunity that allowed regeneration of islet β cells and improvement in glycaemic control [25–28]. However, the success of such interventions is closely related to the time from diagnosis. In many cases, people with long-standing T1D would possess very little to no remaining β cells, and consequently would unlikely be capable of regenerating sufficient quantities of de novo β cells to ameliorate their hyperglycaemia.
MSC modulation of autoimmunity
As previously mentioned, MSCs possess wide-ranging modulatory effects on immune cells, and therefore their use in abrogating autoimmune diseases has been well documented. The autoimmune nature of T1D unsurprisingly ignited interest in the use of MSCs as a potential cell therapy. Several pre-clinical diabetic animal studies showed that transplantation of MSCs results in glycaemic restoration as a consequence of suppressed T-cell proliferation and increased T regulatory cell (Treg) presence within pancreatic islets [14–18]. In a recently published clinical trial, MSCs injected through liver puncture successfully reduced the levels of islet-cell antibodies, glutamic acid decarboxylase, and insulin antibodies of two patients in 12 months, suggesting immune-modulated cell tolerance .
Pancreatic islet and MSC co-transplantation
Alternatively, MSCs recruit and increase the numbers of immunosuppressive host cells during co-transplantation of islets to promote graft survival. Specifically, following human islet transplantation in an advanced humanized mouse model, human bone marrow-derived MSCs (BMSC) increased quantities of Tregs and caused immune tolerance of the transplanted islets . In fact, the co-transplantation of MSCs and pancreatic islets was able to achieve a state of normoglycaemia in diabetic rats likely via MSC trophic factor secretion which protected the transplanted islets . In addition, MSCs in close contact with pancreatic islets began to express Pdx-1 (pancreatic and duodenal homeobox-1, a fundamental transcription factor in β-cell development) and to differentiate into IPCs.
The collective results of these pre-clinical studies [14–18, 20, 30] demonstrate the success of MSCs as an immunomodulatory cell therapy for the treatment of T1D. Nevertheless, solutions are required to overcome the challenge of the loss of immunosuppressive characteristics over time and the requirement for high cell doses. In addition, the fact that animal studies have shown that MSCs derived from diabetic sources were unable to suppress immune responses in animals with diabetes raises concern over the utility of autologously sourced MSCs , potentially limiting the isolation of MSCs to allogeneic sources.
MSCs as targets for the generation of IPCs
Ex vivo generation of IPCs is commonly performed via differentiation of precursor/stem cells using unique chemical regimens [6–10]. Alternatively, IPCs can be generated via viral-mediated gene transfer resulting in the transdifferentiation of lineage-committed cells [3–5]. Currently, encapsulation of IPCs is necessary to prevent recurrent autoimmune destruction or allorejection . Although micro-encapsulation technology for the most part protects IPCs from CD4+ T cell-mediated destruction, these capsules are not impervious to cytokine-induced apoptosis. MSCs are an attractive target for generating IPCs due to their reported immune-modulatory properties. Potentially, IPCs generated from MSCs may retain some MSC-like qualities that could eliminate the need for encapsulation. Currently, there are two methods of generating IPCs from MSCs: chemically induced and viral-mediated transdifferentiation.
Chemically induced transdifferentiation
Deriving IPCs from stem cells has most commonly been performed by adapting in vitro transdifferentiation protocols to yield high numbers of fully functional IPCs [6, 7]. IPCs can be obtained from MSCs via the use of high-glucose culture medium [8, 9] or nicotinamide-enriched medium to induce transdifferentiation . The resulting differentiated cells express insulin at both the mRNA and protein level, and ameliorate hyperglycaemia in STZ rats . Similarly, BMSC isolated for expression of primitive stage-specific embryonic antigen-1 (SSEA-1) and a number of MSC markers such as CXC, octamer-binding transcription factor-4, and stem cell antigen-1 were induced to differentiate into IPCs in defined transdifferentiation medium . These SSEA-1+ cells could further differentiate into islet-like clusters that stained positive with dithizone staining and were capable of secreting insulin in a glucose-responsive manner. MSCs derived from alternative tissue sources have also been subjected to similar chemically defined transdifferentiation protocols that resulted in the successful generation of IPCs [33, 34].
Viral-mediated gene transfer has been investigated as a method to re-introduce normal copies of DNA into abnormal cells, or other cell types, as a means of treating genetic diseases for some time. Ideally, β-cell engineering would employ integrating viral vectors that provide sustained therapeutic gene expression over the life of the patient. To this end, viral-mediated transdifferentiation is an attractive method of obtaining surrogate β cells as they would be less likely to express identical autoantigens against which the primary autoimmune response was developed. Since transcription factors play a significant role in determining islet-cell fate during pancreatic embryogenesis, our laboratory and many others have investigated the direct transfer of β cell transcription factors and insulin as mediators of pancreatic transdifferentiation [3–5, 35].
Due to the success of gene transfer in generating IPCs from a number of cell types, and the requirement for encapsulation to translate basic research into the clinical setting, MSCs have become an attractive target for combinatorial gene and cell therapy. Consequently, several studies were performed investigating the ex vivo targeting of MSCs for viral-mediated transdifferentiation into IPCs. A study by Karnieli et al. utilised retrovirus for the transduction of BMSCs with the key pancreatic transcription factor Pdx-1, resulting in glucose-responsive production of insulin . Furthermore, when these cells were transplanted under the renal capsule of STZ-diabetic SCID mice, they reduced blood glucose levels for a period of 6–8 weeks, after which abnormal glucose tolerance was observed. In fact, Pdx-1 has been delivered to a number of tissue-specific MSCs, including BMSCs  and adipose-derived MSCs [36, 37] with varying success in generating glucose-responsive IPCs. Despite the early success with Pdx-1, some studies have shown that Pdx-1 induced exocrine transdifferentiation, resulting in the development of hepatitis and an increased likelihood of autoimmune destruction . Thus, alternative choices of transcription factors were assessed for their ability to induce pancreatic transdifferentiation without exocrine transdifferentiation. One study in particular found that NeuroD1-betacellulin delivery resulted in no hepatotoxicity, identifying NeuroD1 as an ideal alternative for IPC generation . These results were verified by our laboratory, where the transduction of the H4IIE rat liver cell line with rat NeuroD1 and INS-FUR induced pancreatic transdifferentiation characterised by expression of β cell transcription factors, glucose-stimulated insulin secretion, and reversal of diabetes upon transplantation in STZ-diabetic mice .
Insulin transfer to MSCs has been performed less often, most likely due to the fact that ex vivo transfer of insulin alone is insufficient to induce pancreatic transdifferentiation, and most commonly results in constitutive insulin secretion. Xu et al. studied the retroviral transduction of BMSCs expressing the human insulin gene under the control of the cytomegalovirus (CMV) promoter and the ability of these transduced cells to restore normoglycaemia in STZ-diabetic mice . The results showed that BMSCs successfully expressed insulin and were able to maintain normoglycaemia for at least 42 days. In addition, transduced BMSCs were able to evade autoimmune destruction that ordinarily targets pancreatic islets. However, similar efforts utilising adeno-associated virus (AAV) and retroviral vectors have resulted in constitutive insulin secretion as a consequence of the absence of glucose transporter 2 (GLUT2) and islet glucokinase gene expression. This feature would only develop following transdifferentiation rather than forced insulin expression. Thus, induction of pancreatic transdifferentiation for the production of IPCs should be mediated via transfer of specific pancreatic transcription factors, either alone or in combination, and in addition to the expression of insulin. The success of MSC-derived IPCs in pre-clinical animal models of diabetes is summarised in Table 1.
From pre-clinical success to clinical failure and the way forward
Due to the success of MSC infusions and MSC-derived IPCs in pre-clinical animal models of diabetes, several human clinical trials have been undertaken or are currently in progress (Table 2). In translating pre-clinical studies to the human clinical setting, many of those conducting clinical trials have failed to consider the scientific basis of MSC function that makes their use in the pre-clinical setting so successful. Maintenance of MSC function for successful clinical trials is highly linked to choice of donor, preliminary ex vivo cell culture conditions, cryopreservation, and post-storage culture conditions, four caveats that have been overlooked and are a hallmark of the current clinical failure of MSCs for the treatment of T1D and other diseases.
MSC donor profiling and post-harvest expansion
In many clinical trials, MSCs are harvested from a single donor, culture expanded, and cryopreserved prior to administration to a trial participant. Donor variability is a concern that requires careful assessment prior to harvesting MSCs for clinical trials, as phenotype, age, and gender can affect MSC function and growth characteristics . Consequently, the choice of a suitable donor is critical, as MSCs with a dampened immunomodulatory profile are less likely to perform as well in vivo as MSCs with a robust immunomodulatory profile. As such, profiling of multiple donors for MSC immunomodulatory function should be included as a necessary control step prior to the utilisation of allogeneic MSC treatments.
Compounding single donor harvesting is the fact that MSCs constitute a small proportion of the cellular fraction of the tissue from which they are sourced. Thus, culture expansion is required to generate clinically sufficient quantities of MSCs for transplantation, a process that results in phenotypic changes such as a decrease in proliferative capacity and self-renewal. In addition, doubts concerning the clinical application of ex vivo cultured MSCs for immunomodulatory therapies have been raised due to their apparent loss of immunomodulatory properties with continued subculture (in particular the downregulation of chemokine receptors and reduction in secretion of soluble factors). This is highlighted by the Prochymal® study (Table 2) which pursued the off-the-shelf “one harvest, multiple therapies” concept, and ultimately failed in the clinic due to the extensive ex vivo culture required. The major contributing factor to the success or failure in pre-clinical vs clinical trials of MSCs is that pre-clinical studies utilise freshly harvested MSCs from either single or multiple animal donors followed by immediate transplantation into the animals to be treated. Minor cell expansions are acceptable; however ongoing expansion for upscaling of cell quantities ultimately detrimentally affects MSCs in the clinical setting. Reconstituting the downregulation of MSC chemokine receptors and secreted factors via gene transfer to prolong MSC immunomodulatory properties for clinical therapies is a solution that could prove beneficial, especially with regards to T1D which requires a long-term therapeutic solution. However, multiple gene transfer of exogenous genes represents a logistical challenge that would render such a therapy challenging to translate into the clinic.
Post-cryopreservation culture and administration
Naturally, storage of therapeutic cells is a crucial process in conducting clinical trials. With trial participants required to travel long distances to receive treatment, the need to preserve stocks of therapeutic cells in the interim is imperative. Cryopreservation is the most popular form of cell preservation, and in the clinical setting is a practical form of bio-banking. However, several studies have shown that cryopreserved MSCs have an impaired immunosuppressive profile in comparison to fresh MSCs, explaining the failure of many clinical trials utilising cryopreserved, culture-expanded MSCs [41, 42]. This feature is characterised by increases in expression of heat shock proteins, a lack of interferon (IFN)-γ-mediated indoleamine 2,3-deoxygenase (IDO) production and a reduced immunosuppressive effect on T-cell populations. Interestingly, post-cryopreservation culture for 24 h reverses these effects . As such, successful clinical use of MSCs for the treatment of T1D (and many other diseases) requires careful trial organisation, focusing on the appropriate and responsible use of MSCs. In future, regulation of critical processes such as donor choice, minimal post-harvest expansion, and post-freeze MSC culture could result in more successful clinical trials that fulfil the promise of pre-clinical studies and that of the exciting prospect of MSCs as a cellular therapy for T1D.
CRISPR: a novel strategy to improve MSC therapy for T1D
In recent times, a novel gene-editing technology that has caught the interest of scientists worldwide is the class of RNA-guided endonucleases known as CRISPR-associated protein 9 (Cas9) from the microbial adaptive immune system known as CRISPR [43, 44]. Using short guide (sg)RNAs, the Cas9 endonuclease can be guided to any genomic location, inducing double strand breaks allowing for non-homologous end joining or homologous recombination of genomes at specific locations. By inactivating the catalytic activity of the Cas9 endonuclease, a nuclease-deficient Cas9 (dCas9) can be engineered. In fact, dCas9 fused to transactivation domains such as VP64 or p300 can subsequently be guided using target-specific sgRNAs to the upstream promoter region of endogenous genes, thereby upregulating gene expression [43, 44]. In addition, concerns regarding off-target effects are not as pronounced by comparison to traditional CRISPR applications due to the promoter-specific design of sgRNAs that possess less likelihood of unwanted activator recruitment to promoter regions of non-target genes by chance.
Early studies showed that single sgRNAs targeted to the promoter region of target genes activate negligible levels of endogenous gene expression. However, multiplexing with three or more sgRNAs results in synergistic activation of target genes with significant increases in gene expression. More importantly, sgRNA multiplexing can be designed for multiple gene activation , thereby eliminating the need to deliver multiple transcription factor cDNAs for robust gene expression to induce differentiation. Improvement in dCas9-transcriptional activator fusions have led to novel gene activators with enhanced activation capabilities compared to early dCas9-VP64 activators. Fusions of dCas9 to multiple co-activators such as the VPR domain (consisting of VP64, p65, and Rta), or specifically engineered dCas9 proteins that utilize modified sgRNAs in conjunction with a synergistic activator complex have been shown to induce robust gene activation . In fact, several recent studies have demonstrated CRISPR-mediated activation of endogenous gene expression for controlled differentiation into diverse cell types [47–49].
By utilizing dCas9 activators and multiple sgRNAs to target the endogenous activation of pancreatic transcription factors and/or MSC chemokine receptors in MSCs, it may be possible to direct the differentiation of MSCs into surrogate IPCs capable of maintaining their immunomodulatory properties through ex vivo expansion and transplantation (Fig. 1). To this end, one study in particular has demonstrated success in activating endogenous human insulin transcription utilizing the dCas9-VP160 fusion and multiple insulin promoter targeting sgRNAs in HEK293T, Hela, and human fibroblasts . The success of this study supports the proposed novel application of activating transcription of endogenous genes involved in pancreatic development such as Pdx-1, Neurod1, MafA, etc. When combined with the targeted activation and maintenance of genes involved in MSC immunomodulation such as chemokine receptors and soluble factor production, the likelihood of developing successful MSC-derived therapies for T1D becomes a realistic outcome (Fig. 2).
Both gene therapy and stem cell therapy have proven to be successful avenues for the development of a treatment for T1D. In vitro and in vivo studies have shown that both systems have the potential to be developed further, either individually or in combination. Although MSCs have been shown to be successful in treating T1D by modulating the pancreatic islet microenvironment and immune responses in preclinical and clinical studies, a sustained therapeutic effect is yet to be observed. Similarly, the use of IPCs generated from autologous MSCs to reverse T1D is limited by several challenges such as recurrent autoimmunity, obtaining sufficient numbers of IPCs for transplantation, and loss of immunomodulatory properties. Ultimately, the use of MSC-derived therapies for the treatment of T1D requires appropriate manufacturing conditions that do not impinge on the function of MSCs prior to administration. Clinical success will require perseverance and realistic translation of pre-clinical methodology, without pushing the applicable limits of these therapies.
Bone marrow-derived mesenchymal stem cell
CRISPR-associated protein 9
Cluster of differentiation
Clustered regularly interspaced short palindromic repeat
Glucose transporter 2
Furin cleavable human insulin
- MafA :
V-Maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
Mesenchymal stem cell
- NeuroD1 :
Neurogenic differentiation 1
- Pdx-1 :
Pancreatic and duodenal homeobox-1
short guide RNA
Stage-specific embryonic antigen-1
Type 1 diabetes
- Treg :
T regulatory cell
Atkinson MA, Maclaren NK. The pathogenesis of insulin-dependent diabetes mellitus. N Engl J Med. 1994;19(331):1428–36.
Meloche MR. Transplantation for treatment of type 1 diabetes. World J Gastroenterol. 2007;13:6347–55.
Ren B, O’Brien BA, Swan MA, Koina ME, Nassif N, Wei MQ, et al. Long-term correction of diabetes in rats after lentiviral hepatic insulin gene therapy. Diabetologia. 2007;50(9):1910–20.
Ren B, O’Brien BA, Byrne MR, Ch’ng E, Gatt PN, Swan MA, et al. Long-term reversal of diabetes in non-obese diabetic mice by liver-directed gene therapy. J Gene Med. 2013;15(1):28–41.
Gerace D, Ren B, Hawthorne WJ, Byrne MR, Phillips PM, O’Brien BA, et al. Pancreatic transdifferentiation in porcine liver following lentiviral delivery of human furin–cleavable insulin. Transplant Proc. 2013;45(5):1869–74.
Xie Q-P, Huang H, Xu B, Dong X, Gao S-L, Zhang B, et al. Human bone marrow mesenchymal stem cells differentiate into insulin-producing cells upon microenvironmental manipulation in vitro. Differentiation. 2009;77(5):483–91.
Pagliuca FW, Millman JR, Gurtler M, Segel M, Van Dervort A, Ryu JH, et al. Generation of functional human pancreatic beta cells in vitro. Cell. 2014;159(2):428–39.
Oh S-H, Muzzonigro TM, Bae S-H, LaPlante JM, Hatch HM, Petersen BE. Adult bone marrow-derived cells trans-differentiating into insulin-producing cells for the treatment of type I diabetes. Lab Investig. 2004;84(5):607–17.
Tang DQ, Cao LZ, Burkhardt BR, Xia CQ, Litherland SA, Atkinson MA, et al. In vivo and in vitro characterization of insulin-producing cells obtained from murine bone marrow. Diabetes. 2004;53(7):1721–32.
Wu XH, Liu CP, Xu KF, Mao XD, Zhu J, Jiang JJ, et al. Reversal of hyperglycemia in diabetic rats by portal vein transplantation of islet-like cells generated from bone marrow mesenchymal stem cells. World J Gastroenterol. 2007;13(24):3342–9.
Abouzaripour M, Pasbakhsh P, Atlasi N, Shahverdi AH, Mahmoudi R, Kashani IR. In vitro differentiation of insulin secreting cells from mouse bone marrow derived stage-specific embryonic antigen 1 positive stem cells. Cell J. 2016;17(4):701–10.
Karnieli O, Izhar-Prato Y, Bulvik S, Efrat S. Generation of insulin-producing cells from human bone marrow mesenchymal stem cells by genetic manipulation. Stem Cells. 2007;25(11):2837–44.
Li Y, Zhang R, Qiao H, Zhang H, Wang Y, Yuan H, et al. Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells. J Cell Physiol. 2007;211(1):36–44.
Bassi EJ, Moraes-Vieira PM, Moreira-Sa CS, Almeida DC, Vieira LM, Cunha CS, et al. Immune regulatory properties of allogeneic adipose-derived mesenchymal stem cells in the treatment of experimental autoimmune diabetes. Diabetes. 2012;61(10):2534–45.
Madec AM, Mallone R, Afonso G, Abou Mrad E, Mesnier A, Eljaafari A, et al. Mesenchymal stem cells protect NOD mice from diabetes by inducing regulatory T cells. Diabetologia. 2009;52(7):1391–9.
Klinker MW, Wei C-H. Mesenchymal stem cells in the treatment of inflammatory and autoimmune diseases in experimental animal models. World J Stem Cells. 2015;7(3):556–67.
Ezquer FE, Ezquer ME, Parrau DB, Carpio D, Yanez AJ, Conget PA. Systemic administration of multipotent mesenchymal stromal cells reverts hyperglycemia and prevents nephropathy in type 1 diabetic mice. Biol Blood Marrow Transplant. 2008;14(6):631–40.
Boumaza I, Srinivasan S, Witt WT, Feghali-Bostwick C, Dai Y, Garcia-Ocana A, et al. Autologous bone marrow-derived rat mesenchymal stem cells promote PDX-1 and insulin expression in the islets, alter T cell cytokine pattern and preserve regulatory T cells in the periphery and induce sustained normoglycemia. J Autoimmun. 2009;32(1):33–42.
Sohni A, Verfaillie CM. Mesenchymal stem cells migration homing and tracking. Stem Cells Int. 2013;2013:130763.
Wu H, Wen D, Mahato RI. Third-party mesenchymal stem cells improved human islet transplantation in a humanized diabetic mouse model. Mol Ther. 2013;21(9):1778–86.
Carlsson PO, Schwarcz E, Korsgren O, Le Blanc K. Preserved beta-cell function in type 1 diabetes by mesenchymal stromal cells. Diabetes. 2015;64(2):587–92.
Cai J, Wu Z, Xu X, Liao L, Chen J, Huang L, et al. Umbilical cord mesenchymal stromal cell with autologous bone marrow cell transplantation in established type 1 diabetes: a pilot randomized controlled open-label clinical study to assess safety and impact on insulin secretion. Diabetes Care. 2016;39(1):149–57.
Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Circ Res. 2007;100(9):1249–60.
Hu J, Yu X, Wang Z, Wang F, Wang L, Gao H, et al. Long term effects of the implantation of Wharton’s jelly-derived mesenchymal stem cells from the umbilical cord for newly-onset type 1 diabetes mellitus. Endocr J. 2013;60(3):347–57.
Zhao Y. Stem cell educator therapy and induction of immune balance. Curr Diab Rep. 2012;12(5):517–23.
Zhao Y, Jiang Z, Zhao T, Ye M, Hu C, Yin Z, et al. Reversal of type 1 diabetes via islet beta cell regeneration following immune modulation by cord blood-derived multipotent stem cells. BMC Med. 2012;10:3.
Zhao Y, Jiang Z, Zhao T, Ye M, Hu C, Zhou H, et al. Targeting insulin resistance in type 2 diabetes via immune modulation of cord blood-derived multipotent stem cells (CB-SCs) in stem cell educator therapy: phase I/II clinical trial. BMC Med. 2013;11:160.
Zhao Y, Mazzone T. Human cord blood stem cells and the journey to a cure for type 1 diabetes. Autoimmun Rev. 2010;10(2):103–7.
Mesples A, Majeed N, Zhang Y, Hu X. Early immunotherapy using autologous adult stem cells reversed the effect of anti-pancreatic islets in recently diagnosed type 1 diabetes mellitus: preliminary results. Med Sci Monit. 2013;19:852–7.
Scuteri A, Donzelli E, Rodriguez-Menendez V, Ravasi M, Monfrini M, Bonandrini B, et al. A double mechanism for the mesenchymal stem cells’ positive effect on pancreatic islets. PLoS One. 2014;9(1), e84309.
Fiorina P, Jurewicz M, Augello A, Vergani A, Dada S, La Rosa S, et al. Immunomodulatory function of bone marrow-derived mesenchymal stem cells in experimental autoimmune type 1 diabetes. J Immunol. 2009;183(2):993–1004.
Kirk K, Hao E, Lahmy R, Itkin-Ansari P. Human embryonic stem cell derived islet progenitors mature inside an encapsulation device without evidence of increased biomass or cell escape. Stem Cell Res. 2014;12(3):807–14.
Tsai P-J, Wang H-S, Shyr Y-M, Weng Z-C, Tai L-C, Shyu J-F, et al. Transplantation of insulin-producing cells from umbilical cord mesenchymal stem cells for the treatment of streptozotocin-induced diabetic rats. J Biomed Sci. 2012;19(1):1–9.
Wang HW, Lin LM, He HY, You F, Li WZ, Huang TH, et al. Human umbilical cord mesenchymal stem cells derived from Wharton’s jelly differentiate into insulin-producing cells in vitro. Chin Med J (Engl). 2011;124(10):1534.
Kojima H, Fujimiya M, Matsumura K, Younan P, Imaeda H, Maeda M, et al. NeuroD-betacellulin gene therapy induces islet neogenesis in the liver and reverses diabetes in mice. Nat Med. 2003;9(5):596–603.
Lin G, Wang G, Liu G, Yang LJ, Chang LJ, Lue TF, et al. Treatment of type 1 diabetes with adipose tissue-derived stem cells expressing pancreatic duodenal homeobox 1. Stem Cells Dev. 2009;18(10):1399–406.
Kajiyama H, Hamazaki TS, Tokuhara M, Masui S, Okabayashi K, Ohnuma K, et al. Pdx1-transfected adipose tissue-derived stem cells differentiate into insulin-producing cells in vivo and reduce hyperglycemia in diabetic mice. Int J Dev Biol. 2010;54(4):699–705.
Ren B, Tao C, Swan MA, Joachim N, Martiniello-Wilks R, Nassif NT, et al. Pancreatic transdifferentiation and glucose-regulated production of human insulin in the H4IIE rat liver cell line. Int J Mol Sci. 2016;17(4):534.
Xu J, Lu Y, Ding F, Zhan X, Zhu M, Wang Z. Reversal of diabetes in mice by intrahepatic injection of bone-derived GFP-murine mesenchymal stem cells infected with the recombinant retrovirus-carrying human insulin gene. World J Surg. 2007;31(9):1872–82.
Heathman TRJ, Rafiq QA, Chan AKC, Coopman K, Nienow AW, Kara B, et al. Characterization of human mesenchymal stem cells from multiple donors and the implications for large scale bioprocess development. Biochem Eng J. 2016;108:14–23.
François M, Copland IB, Yuan S, Romieu-Mourez R, Waller EK, Galipeau J. Cryopreserved mesenchymal stromal cells display impaired immunosuppressive properties as a result of heat-shock response and impaired interferon-γ licensing. Cytotherapy. 2012;14(2):147–52.
Moll G, Alm JJ, Davies LC, von Bahr L, Heldring N, Stenbeck-Funke L, et al. Do cryopreserved mesenchymal stromal cells display impaired immunomodulatory and therapeutic properties? Stem Cells. 2014;32(9):2430–42.
Hsu PD, Lander ES, Zhang F. Development and applications of CRISPR-Cas9 for genome engineering. Cell. 2014;157(6):1262–78.
Kabadi AM, Gersbach CA. Engineering synthetic TALE and CRISPR/Cas9 transcription factors for regulating gene expression. Methods. 2014;69(2):188–97.
Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA. Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Res. 2014;42(19), e147.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, et al. Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex. Nature. 2015;517(7536):583–8.
Wei S, Zou Q, Lai S, Zhang Q, Li L, Yan Q, et al. Conversion of embryonic stem cells into extraembryonic lineages by CRISPR-mediated activators. Sci Rep. 2016;6:19648.
Black Joshua B, Adler Andrew F, Wang H-G, D’Ippolito Anthony M, Hutchinson Hunter A, Reddy Timothy E, et al. Targeted epigenetic remodeling of endogenous loci by CRISPR/Cas9-based transcriptional activators directly converts fibroblasts to neuronal cells. Cell Stem Cell. 2016;19(3):406–14.
Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Engineering synthetic gene circuits in living cells with CRISPR technology. Trends Biotechnol. 2016;34(7):535–47.
Gimenez CA, Ielpi M, Mutto A, Grosembacher L, Argibay P, Pereyra-Bonnet F. CRISPR-on system for the activation of the endogenous human INS gene. Gene Ther. 2016;23(6):543–7.
DG is supported by an Australian Postgraduate Award and a scholarship from the Arrow Bone Marrow Transplant Foundation, and was commissioned by the School of Life Sciences, The University of Technology Sydney, to assist with the production of this review. RS is supported by a University of Queensland Vice Chancellor’s Senior Research Fellowship. Research conducted by AMS, NTN, and DG was supported by National Health & Medical Research Council of Australia Project Grants (352909, 513100), project grants from Diabetes Australia Research Trust and Rebecca L. Cooper Medical Research Foundation.
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DG contributed to the review of the literature, writing of the review, and development of the tables and figures which are original to this review. RMW, NTN, SL, and RS contributed to the concept, analysis, and finalisation of the review. AMS contributed to the financial support, concept, analysis, and finalisation of the review. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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