Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration
Stem Cell Research & Therapy volume 7, Article number: 168 (2016)
Tissue-engineering strategies based on mesenchymal stem cells (MSCs) and cell sheets have been widely used for periodontal tissue regeneration. However, given the complexity in periodontal structure, the regeneration methods using a single species of MSC could not fulfill the requirement for periodontal regeneration.
We researched the interaction between the periodontal ligament stem cells (PDLSCs) and jaw bone marrow-derived mesenchymal stem cells (JBMMSCs), and constructed a composite cell sheet comprising both of the above MSCs to regenerate complex periodontium-like structures in nude mice.
Our results show that by co-culturing PDLSCs and JBMMSCs, the expressions of bone and extracellular matrix (ECM)-related genes and proteins were significantly improved in both MSCs. Further investigations showed that, compared to the cell sheet using PDLSCs or JBMMSCs, the composite stem cell sheet (CSCS), which comprises these two MSCs, expressed higher levels of bone- and ECM-related genes and proteins, and generated a composite structure more similar to the native periodontal tissue physiologically in vivo.
In conclusion, our results demonstrate that the crosstalk between PDLSCs and JBMMSCs in cell sheets facilitate regeneration of complex periodontium-like structures, providing a promising new strategy for physiological and functional regeneration of periodontal tissue.
Periodontitis is a common oral disease that leads to the destruction of periodontal tissues, including alveolar bone, periodontal ligament and cementum, and finally to the loosening and loss of teeth . However, existing clinical treatments, such as periodontal flap operation, guided tissue regeneration, and the introduction of certain growth factors, can only clean necrotic tissue and control inflammation, and the restoration of periodontal tissue is limited . Fortunately, in recent years, with the wide application and investigation of tissue engineering in the regeneration and repair of affected tissues, the investigation of stem cells and biomaterials in the biological and functional regeneration of periodontal complex tissue has attracted more attention . To date, stem cells derived from different tissues have been used in animal experiments [4–7] and clinical studies [8, 9].
Since the discovery of the mesenchymal stem cells (MSCs) with multidirectional differentiation capability termed periodontal ligament stem cells (PDLSCs) by Seo et al. in 2004, many experiments have confirmed that this type of stem cell is suitable as the seed cell for the regeneration of periodontal tissue [10, 11]. However, systematic studies have indicated that the use of PDLSCs or progenitor cells from other single tooth sources only regenerate the collagen fiber structure but cannot result in a functional periodontal ligament (PDL) [5, 6]. Whereas in the regeneration experiments for skin, cardiac muscle, and corneal/lens, researchers have activated the signal channels, including the mitigation of cell death, promotion of angiogenesis, and regulation of cellular functions through the interaction between the different stem cells, with satisfactory results having been obtained [12, 13]. In other studies, researchers have co-cultured the MSCs with other types of cells, simulated the interaction of cells from different tissues in the tissue development ex vivo, and induced the occurrence of the cooperative biological behaviors such as migration, proliferation, differentiation, and regulation of cells, thereby providing huge benefits to the regeneration of blood vessels, peripheral nerves, and cartilage [14, 15]. The above research results suggest that the techniques based on the use of single cell types may not be able to meet the requirements for the regeneration of a complex structure such as periodontal tissue.
In recent years, bone marrow-derived mesenchymal stem cells (BMMSCs) have also been widely applied in the tissue-engineering repair of periodontal tissue defects [16, 17]. The research by Hughes et al. shows that this could be achieved through the migration of blood vessels to the surrounding periodontal ligament, allowing it to participate in the formation of periodontal tissue . Recent studies have shown that BMMSCs can not only migrate to the periodontal tissue, and differentiate into related tissue , but also improve the proliferation and differentiation of PDLSCs . Additionally, other studies have confirmed that jaw bone marrow-derived mesenchymal stem cells (JBMMSCs) exhibit more enhanced in situ osteogenicity of alveolar bone than stem cells derived from the ilium , and the bone marrow aspiration-induced pain is much less severe than that of the latter . Both factors have afforded great potential for the use of the tissue-specific BMMSCs in the regeneration of periodontal tissue. Given the above, we hypothesized that the crosstalk between PDLSCs and JBMMSCs could bring benefits to periodontal regeneration.
In addition to the consideration of choice and optimization of seed cells, a strategy for cell delivery should also be emphasized in tissue-engineered regenerative medicine. The delivery of a sufficient number of seeds cells with excellent activity to the defect area  is the first step to ensuring the clinical feasibility of a stem cell-based treatment model . Therefore, a technique called “Cell Sheet Engineering” that uses the extracellular matrix (ECM) secreted during the culture of cells as the endogenous scaffold material has been introduced to the field of periodontal tissue regeneration . This technique avoids the protein damage caused by enzymatic digestion and thus is beneficial to the interaction among the cells and between cells and ECM . As a result, this technique has been applied to the repair of periodontal tissue defects [5, 6]. Previously, our group conducted research on the construction method [27, 28], cell types [29, 30], application strategy [31, 32], and growth factors [20, 33] of cell sheets and achieved some results. However, the regeneration of a complete, fully physiologically functional, complex periodontal structure remains a challenge.
Considering the above research results, we have investigated the mutual effect of co-culturing the PDLSCs and JBMMSCs for the generation of osteoblasts and ECM, based on which a novel cell sheet containing MSCs derived from two different tissue sources was constructed for the regeneration of periodontal tissue. In addition, human treated dentin matrix (hTDM) and calcined bovine bone (CBB), which simulate dental matrix and alveolar bone under physiological conditions, were used as the scaffold materials for the in vivo heterotopic regeneration experiments. We hypothesized that with the interaction between the PDLSCs and JBMMSCs, this composite stem cell sheet would exhibit an enhanced ability to form periodontal-like soft and hard complex structures compared with the cell sheet based on single-variety cells, thus providing a new strategy for the treatment of periodontal tissue defects.
Sample collection and cell culture
hPDLSCs were obtained from the healthy periodontal ligament of premolars extracted from donors (ten donors aged 12–22 years) under orthodontic treatment. hJBMMSCs were obtained from the patients (aged 18–39 years) undergoing orthognathic surgery.
The isolation of hPDLSCs and hJBMMSCs has been described previously [10, 22]. In brief, PDL tissues were gently separated from the surface of the mid-third of the root, and subsequently digested with 3 mg/ml of collagenase type 1 (COL-1, Sigma-Aldrich, St. Louis, MO, USA) for 30 min at 37 °C. Single-cell suspensions were obtained by passing samples through a 70-μm strainer (Falcon, BD Labware, Franklin Lakes, NJ, USA). The cells were seeded into six-well plates and cultured with α-minimum essential medium (α-MEM, Gibco BRL, Gaithersburg, MD, USA) supplemented with 15 % fetal bovine serum (FBS, Gibco, BRL) and incubated in 5 % CO2 at 37 °C. Jaw bone debris was rinsed with α-MEM (Gibco BRL) containing 100 U/mL of penicillin and 100 mg/mL of streptomycin (Invitrogen, Carlsbad, CA, USA) three times. The debris were cut into 1 mm3 pieces and passed through a 70-mm strainer to obtain single-cell suspensions. Both the cell suspensions and jaw bone pieces were seeded into 10-cm dishes and cultured as hJBMMSCs. hPDLSCs and hJBMMSCs at passage three were used for the following experiments.
Single-cell suspensions (2 × 103 cells) within α-MEM (10 % FBS) were seeded in 10-cm-diameter culture dishes (Corning, Lowell, MA, USA) to assess the cell reproductive activity as previous studies [31, 32]. After 14 days of culture the cells were fixed with 4 % paraformaldehyde and stained with 1 % toluidine blue. Aggregates containing ≥ 50 cells were counted as colonies under the microscope and the numbers of colonies per well were counted. The experiment was repeated at least three times.
Single-cell suspensions (1 × 105) within α-MEM (10 % FBS) were seeded in six-well plates. On reaching 80 % confluence, cells were cultured in α-MEM supplemented with adipogenic medium (5 % FBS, 0.5 mM 3-isobutyl-1-methylxanthine, 0.5 mM hydrocortisone, and 100 mg/l indomethacin) or osteogenic medium (5 % FBS, 100 mM dexamethasone, 50 μg/ml ascorbic acid, and 5 mM β- glycerophosphate) for another 21 or 28 days; the medium was changed every 2–3 days. Then the cells were fixed with 4 % paraformaldehyde for 20 min and stained with oil red O or 2 % alizarin red at room temperature. Finally, the cells were routinely observed and photographed under an inverted microscope [31, 32].
Flow cytometric analysis of cell phenotype
For identification of the phenotype, approximately 5 × 105 cells were incubated with PE- or FITC-conjugated monoclonal antibodies against human CD34, CD45, CD90, CD105 (eBioscience, Inc., San Diego, CA, USA), CD29, CD31 and Stro-1 (R&D Systems, Inc., Minneapolis, MN, USA) or isotype-matched control IgGs. For STRO-1 staining, cells were incubated with mouse anti-human STRO-1 for 1 h on ice. After washing with 5 % heat-inactivated FBS, cells were incubated with FITC-conjugated goat anti-mouse IgM (R&D Systems, Inc.) for an additional 30 min on ice. Cells were subjected to flow cytometric analysis with a Beckman Coulter Epics XL cytometer (Beckman Coulter, Fullerton, CA, USA) [31, 32].
The interactions between hPDLSCs and hJBMMSCs in vitro
Indirected co-culture of hPDLSCs and hJBMMSCs
Transwell chambers with 0.4-μm pores were used to examine the interactions of hPDLSCs and hJBMMSCs . hPDLSCs/hJBMMSCs were seeded in six-well plates at a density of 2.5 × 105/ml, which were covered with Transwell chambers. Correspondingly, hJBMMSCs/hPDLSCs were seeded in chambers. Then, hPDLSCs and hJBMMSCs were co-cultured reciprocally in α-MEM medium with 10 % FBS as experimental groups, while they were co-cultured with themselves respectively as control groups.
The co-cultured cells were treated with osteogenic medium for 14–28 days, then washed twice in PBS after fixation in 4 % paraformaldehyde for 30 min in 4 °C. Calcium accumulation was detected at day 28 by 2 % alizarin red staining and dissolved by 1 mL of sodium dodecyl sulfate solution. The light absorption of sodium dodecyl sulfate solution with alizarin red was read at 570 nm with a microplate reader (Bio-TEK Instruments, Inc., Winooski, VT, USA). Alkaline phosphatase (ALP) staining and activity were determined at day 14 with the BCIP/NBT Alkaline Phosphatase Color Development Kit (Beyotime Co., Shanghai, China) and Alkaline Phosphatase (AKP/ALP) Detection Kit (Zhong Sheng Co., Beijing, China) . The experiment was repeated at least three times.
Real-time polymerase chain reaction (RT-PCR) assay and Western blot (WB) assay
RNA from co-cultured cells was split, collected, purified and extracted with Trizol Reagent (Invitrogen) according to the manufacturer’s protocol. The cDNA synthesis and PCR were performed as previous described [31, 32]. The primer sequences for ALP, COL-1, bone sialoprotein (BSP), osteocalcin (OCN), fibronectin, integrinβ1, periostin and β-actin are listed in Table 1. β-actin was used as an internal control. The PCR program was 94 °C for 5 min, followed by 35 cycles of 94 °C for 45 s, 57 °C for 45 s, and 72 °C for 1 min, and a final extension step at 72 °C for 1 min. This experiment was repeated three times, and the PCR products were further confirmed by sequencing (Sangon Biotechnology Co., Shanghai, China).
Total cellular protein was extracted from co-cultured cells after 7 days of culture with lysis buffer (pH 8.0) containing 1 % NP-40 (Sangon Co., Shanghai, China), 50 mM Tris(hydroxymethyl)aminomethane (Tris)-hydrogen chloride (HCl), 150 mM sodium chloride (NaCl), 0.1 mM phenylmethylsulfonyl fluoride, and 1 mg/ml aprotinin (Sigma-Aldrich) [31, 32]. We determined the protein concentration in the extracted lysates by measuring the absorbance at 595 nm with a protein assay solution (Bio-Rad, Hercules, CA, USA). Aliquots of 20–50 μg of cell lysate were separated by 10 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred to a polyvinylidene fluoride (PVDF) membrane (Bio-Rad). The membranes were blocked with 5 % milk for 2 h and then incubated with primary antibodies overnight. The immune complexes were incubated with horseradish peroxidase-conjugated anti-goat or anti-mouse IgG antibodies (Boshide, Beijing, China). Immunodetection was performed with the Western-Light chemiluminescent detection system (Peiqing, Shanghai, China). Primary antibodies were purchased from the following commercial sources: polyclonal antibodies against runt-related gene 2 (RUNX2), alkaline phosphatase (ALP), osteocalcin (OCN), periostin, integrinβ1, fibronectin from Abcam, (Cambridge, UK) monoclonal antibodies against bone sialoprotein (BSP) and collagen I (COL-1) from Santa Cruz Biotechnology (Dallas, TX, USA), and monoclonal antibodies against β-actin from Cell Signaling Technology (Danvers, MA, USA).
Evaluation of three different types of cell sheets in vitro
Construction strategy of different cell sheets
Multiple colony-derived hPDLSCs, hJBMMSCs and the mixed cells of the two in equal proportions at passage 3 were seeded at approximately 1 × 105/mL into six-cell plates, and cultured in normal α-MEM medium containing 10 % FBS for 3 days (Additional file 1A). After reaching 90 % confluence, the medium was changed into the α-MEM medium, containing 10 % FBS and ascorbate (50 μg/ml). After 9–10 days, the cell sheets, including periodontal ligament stem cell sheet (PDLSCS), jaw bone marrow-derived mesenchymal stem cell sheet (JBMMSCS) and composite stem cell sheet (CSCS), were formed and easily detached from the culture plates with a cell scraper (Additional file 1B-D) [31, 32].
The attached cell sheets were washed twice in PBS after fixation in 4 % paraformaldehyde for 30 min in 4 °C. Alizarin red staining and ALP staining were performed and quantified as before. The experiment was repeated at least three times.
Real-time polymerase chain reaction (RT-PCR) assay and Western blot (WB) assay
The expression of gene and protein (ALP, COL-1, RUNX2, BSP, OCN, periostin, fibronectin, and integrinβ1) were detected in the exactly same way as before.
Hematoxylin and eosin (H&E) and immunohistochemical staining
The detached cell sheets were fixed in 4 % phosphate-buffered paraformaldehyde for 24 h, paraffin-embedded, longitudinally sectioned and stained with H&E as previous described [31, 32]. Other sections were incubated with primary antibodies following anti-ALP (1:200, Abcam), anti-COL-1 (1:200, Santa Cruz Biotechnology), anti-fibronectin (1:200, Abcam,), anti-periostin (1:200, Abcam), and anti-integrinβ1 (1:200, Abcam). PBS was used for the negative controls instead of the primary antibodies. Biotinylated secondary antibodies (1:1000) were purchased from Dako (Dako, Santa Clara, CA, USA). The staining sections were observed with a light microscope (Nikon, Tokyo, Japan).
Scanning electron microscopy (SEM) observation
The detached cell sheets were fixed by 4 % paraformaldehyde. The samples were anodized in an electrolyte containing 0.5 wt% hydrofluoric acid and 1 M phosphoric acid for 1 h. After that, the whole complex was observed by scanning electron microscope (SEM) (Hitachi, S-4800, Tokyo, Japan).
Preparation of ceramic bovine bone (CBB) and human treated dentin matrix (hTDM)
Human treated dentin matrix preparation
The procedure for the preparation of hTDMs was described previously . Briefly, premolar teeth, removed for clinical reasons at the School of Stomatology, Fourth Military Medical University, were collected. Periodontal tissue was completely scraped away using a curette along with removal of the outer cementum and part of the dentin. Dental pulp tissues and pre-dentin were also mechanically removed. For the fabrication of hTDM, the dentin matrix was formed to a length of 5.0 mm and a thickness of up to 2.0 mm and mechanically cleaned using an ultrasonic cleaner. Human dentin matrices were then treated with 17 % ethylenediaminetetraacetic acid (EDTA, Sigma-Aldrich) for 5 min, 10 % EDTA for 5 min, 5 % EDTA for 10 min. hTDMs were maintained in sterile PBS with 100 unit/ml penicillin (Hyclone, Logan, UT, USA) and 100 mg/ml streptomycin (Hyclone) for 72 h, then washed in sterile deionized water for 10 min in an ultrasonic cleaner, finally stored in α-MEM at 4 °C.
Finally, the mixed cells, including hPDLSCs and hJBMMSCs, were seeded onto the prepared hTDM and incubated in normal α-MEM medium containing 10 % FBS for 7 days to inspect the cellular compatibility of the scaffold.
Ceramic bovine bone preparation
The ceramic bovine bone (CBB) was produced from fresh bovine rib bones, which was subsequently cut into blocks, washed in normal saline and soaked in H2O2 to remove proteins. Next, the blocks were formed to a length of 5.0 mm and a thickness of up to 2.0 mm. These blocks were washed with running water, heated at 900 °C for 1 h, and sterilized in a high temperature and pressure environment. Then they were washed in sterile deionized water for 10 min in an ultrasonic cleaner, finally stored in α-MEM at 4 °C .
Finally, the mixed cells, including hPDLSCs and hJBMMSCs, were seeded onto the prepared CBB and incubated in normal α-MEM medium containing 10 % FBS for 7 days to inspect the cellular compatibility of the scaffold.
Observation of the hTDM/CBB surface via scanning electron microscopy) analysis
The hTDM/CBB with/without cells was fixed by 4 % paraformaldehyde. The samples were anodized in an electrolyte containing 0.5 wt% hydrofluoric acid and 1 M phosphoric acid for 1 h. After that, the whole complex was observed by scanning electron microscope (SEM) (Hitachi, S-4800) [31, 32].
In vivo transplantation
Nude mice ectopic transplantation
The hTDMs/CBBs coated respectively by different types of cell sheets were kept in the wells of 12-well plates with a minimal amount of cell culture medium as grafts (Additional file 1E, F). Eighteen 6-week-old immunodeficient mice (BALB/c-nu; FMMU Medical Laboratory Animal Center, Xi’an, China) were used in this experiment. Mice were anesthetized, and the dermal space was created by blunt lateral dissection from a single dorsal midline incision. Each mouse received two grafts, one on each side. The wounds were sutured to achieve primary closure (Additional file 1G) [31, 32]. Six weeks after transplantation, the mice were euthanized, and the grafts were removed for histological analysis.
Morphological and histomorphometric evaluation of regenerated PDL tissues
The samples were fixed in 4 % paraformaldehyde overnight at 4 °C, decalcified with 17 % EDTA (pH 8.0), embedded in paraffin, and cut into 5-μm sections. For histological analysis, sections were stained with hematoxylin and eosin (H&E) and Masson’s Trichrome as previous described [31, 32]. Deparaffinized sections were immersed in 3 % H2O2/methanol for 15 min to quench endogenous peroxidase activity and incubated with primary antibodies (1:100 to 1:500 dilutions) overnight at 4 °C. Polyclonal antibodies against human cementum protein 1 (CEMP1) and BSP from Abcam were used as primary antibodies. Isotype-matched control antibodies were used under the same conditions as the primary antibodies. Sections were counterstained with hematoxylin. A microscope (Leica Microsystems AG, Wetzlar, Germany) was used for histological evaluation, which was based on the morphological observation of three sections per complex.
All results are representative of data generated in three independent experiments. All of the statistical analyses were performed using ANOVA followed by Fisher’s protected least significant difference (PLSD) test and Student’s t test by SPSS version 15.0 software (SPSS, Inc., Chicago, IL, USA). All of the values are expressed as mean ± SD. A P value <0.05 was considered to be statistically significant. All procedures were performed blind.
Isolation and characterization of hPDLSCs and hJBMMSCs
The primary hPDLSCs/hJBMMSCs were seen around the tissue pieces (Fig. 1a, h), and retained their fibroblast spindle shape after passage (Fig. 1b, i). When they were cultured at a low density, they formed adherent clonogenic cell clusters (colony-forming unit, fibroblastic, CFU-F) (Fig. 1d, k). The colony formation efficiency of hPDLSCs/hJBMMSCs was 27.3 % and 25.7 % respectively (Fig. 1c, j).
After culturing in adipogenesis-inducing medium for 21 days, hPDLSCs/hJBMMSCs both were observed by oil red staining and were found to form lipid droplets (Fig. 1e, l). After induction in osteogenesis medium for 21 days, they were both observed with alizarin red staining and were found to form mineralized nodules (Fig. 1f, m).
hPDLSCs/hJBMMSCs both exhibited a characteristic pattern of mesenchymal surface markers, including CD90, CD105, CD29 and STRO-1, whereas the hematopoietic markers CD31, CD34 and CD45 were negative (Fig. 1g, n).
The crosstalk between hPDLSCs and hJBMMSCs in vitro
To investigate the mutual effect between hPDLSCs and hJBMMSCs in osteogenic capability, they were co-cultured by Transwell method in osteogenic differentiation media. The results of alizarin red staining and ALP staining both showed that the co-cultured stem cells could form more mineralization nodules and exhibit higher ALP activity, compared with corresponding controls (Fig. 2a, b). To further analyze, real-time PCR was conducted, which demonstrated that the osteogenesis- and ECM-related gene expression of ALP, COL-1, RUNX2, BSP, OCN, fibronectin, integrinβ1, and periostin was much higher in co-cultured cells than cells in control groups (Fig. 2e, f). Meanwhile, the same trend was observed in the protein expression levels (Fig. 2c, d). These data indicated that the interactions between hPDLSCs and hJBMMSCs might promote their osteogenic differentiation potential and capability of ECM formation.
Evaluation of three different types of cell sheets in vitro
To investigate the difference of PDLSCS, JBMMSCS, and CSCS in osteogenic capability, they were cultured in osteogenic differentiation media. The results of alizarin red staining and ALP staining both showed that CSCS could form more mineralization nodules and exhibit higher ALP activity, compared with the other two (Fig. 3a, b).
Furthermore, real-time PCR showed that the osteogenesis- and ECM-related genes expression of ALP, COL-1, RUNX2, BSP, OCN, Fibronectin, Integrinβ1, and Periostin, was much higher in CSCS than PDLSCS/JBMMSCS (Fig. 3d). And the same trend was observed in the protein expression levels (Fig. 3c).
Additionally, the SEM analysis demonstrated all three cell sheets established a film-like cell network that retained tight junctions between cells, while among the three cell sheets, CSCS contained the most compacted cell arrangement and collagen secretion (Fig. 4a). H&E staining and immunohistochemical staining showed that all three cell sheets were dense and contained plenty of cells (Fig. 4b), and all three cell sheets positively expressed ALP (Fig. 4c), BSP (Fig. 3d), COL-1 (Fig. 4e), fibronectin (Fig. 4f), integrinβ1 (Fig. 4g), OCN (Fig. 4h), periostin (Fig. 4i) and RUNX2 (Fig. 4j), while the staining intensity of all the proteins in CSCS increased notably, compared with the other two cell sheets. These data suggested that the interactions between different kinds of cells during the formation of cell sheets may result in CSCS possessing more capability in osteogenic differentiation and ECM secretion than other two cell sheets.
Regenerative PDL-like tissue formation around scaffolds surface in vivo
CBB and hTDM were chosen to simulate the two kinds of hard tissues, which are anatomically located on either side of the periodontal membrane in normal physiological conditions. SEM analysis of the scaffold CBB showed a porous structure (Additional file 2A, B). In contrast, the surface of hTDM showed dentinal tubules were sufficiently exposed after being treated with EDTA (Additional file 2E, F). The SEM images also showed that both hPDLSCs and hJBMMSCs could adhere to the scaffolds well, proliferate adequately, and extend excessively on the surface of hTDM and CBB (Additional file 2C, D, G, H).
At the end of 6 weeks after transplantation of the cell sheets into the subcutaneous space of the immunodeficient mice, we harvested all 36 regenerated tissue specimens and examined their morphology. H&E and Masson Trichrome staining showed that an amount of dense, ordered fibers were closely adhered to the surface of the CBB, with notable mineralized deposition, in the CSCS group (Fig. 5a-d). Whereas plenty of cluttered fibers were formed in the PDLSCS group (Fig. 5e-h), and in the JBMMSCS group, the regenerated fibers were sparse and unordered, although there was a deposition of osteoids (Fig. 5i-l).
On the other hand, typical arranged tissue with Sharpey-like fibers was regenerated, which was closely attached to the surface of hTDM at an oblique or horizontal angle in the CSCS group (Fig. 6a-d). In contrast, in the PDLSCS group, few inserted fibers were observed, and most of the fibers were parallel with the surface of the scaffolds (Fig. 6e-h), and in the JBMMSCS group, there were a few collagenous fibers, sparsely and chaotically arranged, in regenerated tissues (Fig. 6i-l). Furthermore, immunohistochemical staining showed that the expressions of CEMP1 and BSP were much higher in the CSCS group (Fig. 6m, n) than in the two other groups (Fig. 6o-r), especially in the juncture of soft and hard tissues. Taken together, the CSCS, which regenerated PDL-like tissue not only on the CBB, but also on the hTDM, may be more appropriate for periodontal regeneration than the PDLSCS/JBMMSCS.
In recent years, to overcome the inability of traditional periodontal treatment methods to regenerate functional periodontal tissue , the tissue-engineering repair of periodontal tissue based on the use of stem cells has received widespread attention and been the subject of more in-depth investigations . Furthermore, the cell sheet technique, a cell delivery strategy that retains the autocrine function of the ECM in the same cells, has been incorporated into more and more applications in the study of tissue-engineering repair of periodontal tissue [25, 34], and cell sheets constructed of cells derived from different tissues have been used in the repair of periodontal tissue defects in animals, and certain effects have been reported [5, 35]. However, the use of a single type of cell cannot reliably regenerate periodontal tissue, a complex structure containing both soft and hard tissues. Among the stem cells that have been used in the regeneration of periodontal tissue, the PDLSCs and BMMSCs have attracted more attention for their potential in the regeneration engineering of periodontal tissue because of their strong proliferation capability and ability to differentiate into periodontal tissue, as well as their wide availability [3, 23]. In addition, previous research has also found that the MSCs derived from the jaw exhibited a stronger potential to form periodontal complex tissue than MSCs derived from the ilium [21, 36]. Therefore, the present study utilized PDLSCs and JBMMSCs, two types of MSCs derived from tissues closely related to periodontal tissue. In addition, the interaction between these two types of MSCs has been investigated, and a novel cell sheet comprising these two, which are derived from different tissues, has been constructed. This composite stem cell sheet, which exhibits unique biological characteristics, is capable of forming complex periodontal-like soft and hard tissue structures in subcutaneous areas of nude mice, providing a new possibility for the treatment of periodontal tissue defects.
The regeneration of functional periodontal-like tissue that contains both soft and hard tissues is the ultimate goal of the tissue-engineering repair of periodontal tissue defects. Among these, the regeneration of bone tissue in specific locations is particularly important because not only does bone tissue provide the most important structural basis for the stability of the tooth root, but it also generates Sharpey’s fiber. This fiber is a unique dense connective tissue in periodontal tissue that perforates soft and hard tissues and connects the cementum of the tooth root to the alveolar bone. This connective tissue is required for the proper functioning of periodontal tissue. Similar to the formation of bone tissues in other locations, the bone matrix-related proteins that are synthesized and secreted by the same cells are the basis for the accumulation of calcium and thus the mineralization of tissue . Therefore, in this experiment, we measured the levels of ALP, OCN, BSP, RUNX2, and periostin, which are expressed in normal periodontal tissue and participate in the mineralization and osteogenesis of tissue at different stages from the cellular level and the cell sheet level. The results showed that the expression of the genes and proteins for the aforementioned materials was higher than that in the control groups. The staining of the calcium nodule, the activity of the ALP, and the immunohistochemistry of paraffin sections of the cell sheet (IHC-P) also confirmed this finding. Therefore, we believe that the PDLSCs and JBMMSCs can enhance each other’s capacity for osteogenic differentiation. Moreover, compared to the cell sheets comprising a single cell type, the cell sheet that comprises these two types of stem cells shows stronger osteogenic potential, which is beneficial to the functional regeneration of periodontal tissue. Furthermore, the expression of the ECM-related genes and proteins in the experimental group, including the COL-1, fibronectin, integrinβ1, that closely participate in cell adhesion, migration, proliferation, differentiation, and apoptosis  was significantly increased, suggesting that the cell sheet constructed from the indirect or direct co-culture exhibited stronger ability to synthesize and secrete ECM. In the cell sheet technology, the ECM secreted by the same cells forms a biochemical and mechanical cell niche. The various factors within the cell niche affect the cellular activity; coordinate the interactions among the cells, the soluble cellular factors, and the ECM ; and regulate the structure and function of the newly regenerated tissue. Therefore, enhancing the formation and preservation ability of the ECM by seed cells is beneficial to the complete regeneration of the periodontal tissue.
In the regeneration of complex tissues, the importance of microenvironment in the regulation of seed cells has been confirmed . The various cellular factors in the microenvironment can enhance the regeneration of complex tissues by affecting the migration, proliferation, and differentiation of the stem cells [41, 42]. However, a continuous and effective stimulation of the seed cells is often lacking in the microenvironment of the in vivo defects or in the areas requiring regeneration. The factors secreted by a single cell type are limited and cannot completely improve the microenvironment in the defect locations, thus resulting in pronounced apoptosis , deficiency of vascularization , or incompleteness of the structure of the regenerated tissue [44–46]. Therefore, the construction of a physiologically similar microenvironment to that which produces and maintains the periodontal ligament is important. Previous studies have focused on the change in the gene expression and protein secretion of the seed cells through gene transfection  or on the provision of beneficial environments to seed cells by using the cell culture supplements derived from plasma that is rich in growth factors  or conditioned medium derived from embryonic cells . However, all these methods have limitations. We found that the results of previous studies suggest that the progenitor cells in the periodontal ligament might come from the marrow cavity and reach the periodontal tissue via the circulation of the blood . The MSCs in the periodontal ligament on the alveolar side showed stronger multidirectional differentiation capability and expressed a higher level of osteogenesis-related proteins than those on the cement side . Taking into account the intimate affinity of the two tissues  and their interaction in the development process , we speculated that the interaction between the PDLSCs and JBMMSCs plays an important role in the formation and stability of the unique microenvironment of the periodontal tissue. Therefore, in this study, we directly mixed these two types of MSCs to construct the cell sheet and reconstruct the interaction of the two types of stem cells under physiological conditions, while simultaneously forming an ECM that contained multiple factors beneficial to in vivo regeneration. Compared to previous methods, the stable and direct relation among the cells established through co-culture gives rise to a more sustainable, comprehensive, and safe simulation and effect on the target cells. In addition, the resultant change in the gene and protein levels compensates for the signal molecules that were lost in the ex vivo culture . All of these factors positively contribute to the regeneration of complex tissues. The in vivo heterotopic regeneration experiments in nude mice also confirmed our hypothesis. Whether on the surface of hTDM or CBB, the composite cell sheet comprising two types of stem cells formed a large amount of a dense and ordered periodontal-like composite structure that closely connects the osteoid and scaffolding materials, with high expression of CEMP1 and BSP, which play vital roles in the growth and development of periodontal and cementum tissue [3, 55]. However, the regenerated tissue from a single cell type exhibited defects in fiber density, direction, and the accumulation of new bone. Besides, the species reactivity of the antibodies used in our experiments is human, which indicates the origin of regenerated tissues.
Although we have observed the biologically functional benefits from the interaction of the MSCs in the ex vivo experiment and successfully regenerated periodontal-like composite tissue structure in the in vivo heterotopic experiment, some problems remain to be resolved, e.g., the mechanism of the interaction between the two cell types, and the fate of the implanted cells in vivo. Recent studies on exosomes  and Gabriel Rahmi’s latest study  revealed that although most of the implanted cells would die in a few days, they could be involved in tissue repair through the paracrine effect, which offer us some insights regarding future investigations. In addition, clinical periodontal tissue defects normally are accompanied by inflammation, whether the composite can properly function in this microenvironment remains unknown. These questions will be the focus in our future studies.
In summary, the composite cell sheet comprising two types of MSCs derived from two closely related tissues provide a more suitable microenvironment for the regeneration of periodontal tissue with complex structure. This structure arises from the interaction of the two types of MSCs, thus providing a promising new strategy for the clinical repair of periodontal tissue defects.
bone marrow-derived mesenchymal stem cells
ceramic bovine bone
cementum protein 1
composite stem cell sheet
fetal bovine serum
hematoxylin and eosin
human treated dentin matrix
immunohistochemistry paraffin protocol
jaw bone marrow-derived mesenchymal stem cells
jaw bone marrow-derived mesenchymal stem cell sheet
mesenchymal stem cells
periodontal ligament stem cell sheet
periodontal ligament stem cells
real-time polymerase chain reaction
runt-related gene 2
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
scanning electron microscopy
α-minimum essential medium
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The authors would like to acknowledge Hua Ni and Yuyun Bai for their assistance with sample collection and histological preparations.
This work was supported by grants from the Nature Science Foundation of China (81470710, 81470679, 81300718 and 81271065).
Availability of supporting data
HZ carried out all the experiments, participated in the design of the study, and drafted the manuscript. SL participated in the design of the study, performed the statistical analysis, and helped to revise the manuscript. BZ carried out the in vivo transplantation experiment and acquisition of the data, and drafted the manuscript. QX carried out the culture and identification of cells, and revised the manuscript. YD was involved in revising the manuscript. YJ participated in the design of the study, interpretation of data, and revising the manuscript. All authors read and approved the manuscript.
The authors declare that they have no competing interests.
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Ethics approval and consent to participate
The experimental protocols were approved by the ethics committee (Institutional Review Board for Human Subjects Research) of the School of Stomatology, Fourth Military Medical University (FMMU), and all donors or guardians provided written informed consent for the donation of their removed teeth and subsequent use in this research, the serial number is IBR-REV-2014003.
All animal experiments were conducted in accordance with the committee guidelines of the Fourth Military Medical University for animal experiments, which meet National Institutes of Health (NIH) guidelines for the care and use of laboratory animals.
Construction strategy of different cell sheets and nude mouse ectopic transplantation. (A) Multiple colony-derived hPDLSCs, hJBMMSCs, and the mixed cells of the two above in equal proportions were seeded into six-cell plates, and after induction by conditioned media, (B) PDLSCS, (C) JBMMSCS, and (D) CSCS were formed. (E, F) CBB/hTDM wrapped by cell sheets was cultured as the transplantation grafts. (G) Each mouse received two grafts, one on each side. The wounds were sutured to achieve primary closure. (PDF 596 kb)
Microscopic appearance of the scaffold materials and the adhesion of the cells to the scaffolds. (A, B) CBB showed a porous structure, and (E, F) the surface of hTDM showed dentinal tubules were sufficiently exposed. (C, D, G, H) The hPDLSCs and hJBMMSCs could adhere to these two scaffolds well, proliferate adequately, and extend excessively on the surface of CBB and hTDM. (PDF 511 kb)
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Zhang, H., Liu, S., Zhu, B. et al. Composite cell sheet for periodontal regeneration: crosstalk between different types of MSCs in cell sheet facilitates complex periodontal-like tissue regeneration. Stem Cell Res Ther 7, 168 (2016). https://doi.org/10.1186/s13287-016-0417-x